Dressing prepared by using freeze-dried calcium alginate/vaterite calcium carbonate composite microspheres and preparation method thereof

A technology of composite microspheres and calcium alginate, applied in medical science, bandages, etc., can solve the problems of easy infection of wounds, slow recovery speed, etc., to promote repair and healing, promote repair and healing of damaged tissues, and avoid frequent dressing changes. Effect

Active Publication Date: 2018-10-23
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if the exudate of the wound surface is too much, resulting in the wound surface being too moist, it is easy to cause the wound to be easily infected and the recovery speed is too slow

Method used

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  • Dressing prepared by using freeze-dried calcium alginate/vaterite calcium carbonate composite microspheres and preparation method thereof
  • Dressing prepared by using freeze-dried calcium alginate/vaterite calcium carbonate composite microspheres and preparation method thereof
  • Dressing prepared by using freeze-dried calcium alginate/vaterite calcium carbonate composite microspheres and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A kind of dressing that utilizes freeze-dried calcium alginate / vaterite calcium carbonate composite microspheres to prepare, antibacterial drug in the present embodiment is rifamycin sodium as an example, cell growth factor is mouse basic fibroblast growth factor, The dressing is prepared as follows:

[0041] (1) Co-precipitation method to prepare vaterite calcium carbonate suspension: add Na at a concentration of 50mM to a beaker with a capacity of 100mL 2 CO 3 + 8mg / mL casein 20mL, magnetic stirring at 600rpm, magnet diameter 40mm, at the same time add 20mL of 50mM CaCl in the separatory funnel 2 , Open the cock just above the beaker and add evenly. Magnetic stirring was carried out for 20 min. After the stirring was completed, the carbonate ions and calcium ions in the solution completely reacted to obtain CaCO 3 Suspension, i.e. vaterite calcium carbonate suspension, in which CaCO 3 The concentration is 2.5mg / mL.

[0042] (2) Prepare rifamycin sodium solution:...

Embodiment 2

[0053] (1) According to the steps in Example 1, a dressing prepared by using freeze-dried calcium alginate / vaterite calcium carbonate composite microspheres was prepared. In order to facilitate observation and detection, trypan blue (dye ) instead of antibacterial drugs or cell growth factors as loaded drugs, observe the prepared freeze-dried composite microspheres, and its morphology under an optical microscope is as follows figure 2 shown. The light microscope image of the freeze-dried composite microspheres after re-swelling is shown in image 3 shown.

[0054] From figure 2 and image 3 It can be seen from the figure that after lyophilization, the composite microspheres re-swelled to form a hydrogel state, and their spatial structure was more plump and three-dimensional, which did not change much compared with that before lyophilization.

[0055] (2) Referring to the method and steps in (1), trypan blue was used instead of antibacterial drugs or cell growth factors a...

Embodiment 3

[0059] The antibacterial effect of the composite microspheres loaded with drugs was tested as follows:

[0060] (1) Staphylococcus aureus was inoculated in the culture medium (LB, 1% peptone, 0.5% yeast extract and 1% NaCl), and shaken at 200rpm at 37°C for 24h. Take a portion of the bacterial suspension and dilute it using the gradient method. The bacterial concentration after counting is 8*10 8 CFU / mL. Draw 100 μL and spread evenly on the LB agar medium in the 90mm Petri dish.

[0061] (2) Taking composite microsphere A prepared in Example 1, composite microsphere A and composite microsphere B mixed at a ratio of 1:1 as the test object, the composite microsphere Place A and composite microsphere C on a 90mm filter paper sheet, then transfer the filter paper sheet to the center of the medium in step (1), and culture for 24 hours. In order to analyze the sustained antibacterial effect of the experimental group, the above-mentioned filter paper pieces were transferred to a ...

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Abstract

The invention discloses a dressing prepared by using freeze-dried calcium alginate / vaterite calcium carbonate composite microspheres and a preparation method thereof. The dressing comprises compositemicrospheres loaded with a bacteriostatic drug and composite microspheres loaded with a cell growth factor, and the mass ratio of the composite microspheres loaded with the bacteriostatic drug to thecomposite microspheres loaded with the cell growth factor two is 1:1-5. The composite microsphere is prepared by uniformly mixing a uniformly dispersed vaterite calcium carbonate suspension and a sodium alginate solution and then using a microfluidic device. By using the calcium alginate / codbitite calcium carbonate composite microsphere as a release carrier, release period of the loaded drug and cell growth factor exceeds a week, thus avoiding frequent dressing change. In the early stage of wound healing by the use of the dressing, the freeze-dried calcium alginate layer blocks the bleeding site, the wound exudates is effectively absorbed, and the release of the bacteriostatic drug prevents bacteria breeding from infecting the wound. The growth factor is slowly and smoothly released duringthe whole period of wound recovery, thereby achieving the functions of stopping bleeding, inhibiting bacteria and promoting the repair and healing of wounded tissues.

Description

technical field [0001] The present invention relates to the technical field of biomedical material drug sustained-release microspheres and biomaterial modified antibacterial to promote wound healing, more specifically, relates to a dressing prepared by using freeze-dried calcium alginate / vaterite calcium carbonate composite microspheres and its preparation method. Background technique [0002] Wound healing is a complex repair process, and its healing process can be roughly divided into three stages: coagulation, inflammation, migration and proliferation of epidermal cells, and formation and remodeling of the epidermis. The long-term high exposure of the wound will destroy the barrier function of the skin, and the necrotic tissue can also promote the reproduction and spread of many pathogens, resulting in repeated wound infections. At the same time, the bacterial immune response will further prolong the tissue inflammation time, weeks or months after the injury The wound is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00
CPCA61L26/0004A61L26/0023A61L26/0066A61L2300/252A61L2300/404A61L2300/414A61L2300/45A61L2300/602C08L5/04
Inventor 李燕史明张伊玲高芸芬刘筱芳轩留洋
Owner SUN YAT SEN UNIV
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