A kind of microbial liquid fertilizer containing bacillus and its preparation method and application

A Bacillus and Bacillus licheniformis technology, applied in the field of agricultural fertilizers, can solve problems such as single function and poor storage stability, and achieve the effects of increasing yield, improving tea quality, and improving soil environment and microbial flora.

Active Publication Date: 2020-07-24
INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a microbial liquid fertilizer containing bacillus and its preparation method and application, so as to solve the problems of single function and poor storage stability in the existing microbial liquid fertilizer

Method used

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  • A kind of microbial liquid fertilizer containing bacillus and its preparation method and application
  • A kind of microbial liquid fertilizer containing bacillus and its preparation method and application
  • A kind of microbial liquid fertilizer containing bacillus and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Obtaining and Identification of Bacillus licheniformis W205 and Bacillus subtilis W309

[0024] 1. Strain isolation

[0025] Soil samples were collected from farmland in Shoushan Village, Shoushan Township, Jin'an District, Fuzhou City, Fujian Province. Weigh 5g of soil sample into a sterilized centrifuge tube, add 5 times the volume of sterile water, filter the soil, take the filtrate and inoculate it on LB plate medium, and cultivate it at a constant temperature at 37°C until colonies appear, then pick it up with an inoculation needle Bacteria that grow well on the edge were inoculated on a new LB plate medium. After the newly inoculated endophytic bacteria grew into colonies, the endophytic bacteria on the edge were picked and cultured by streaking. Purification was repeated in this way, and a total of 18 endophytic bacteria were obtained. For single colonies, select two of them and name them W205 and W309, respectively.

[0026]The LB plate medium includ...

Embodiment 2

[0033] The preparation of embodiment 2 microbial liquid fertilizer

[0034] (1) Preparation of LB medium: peptone 10g / L, yeast extract 5g / L, sodium chloride 10g / L, adjust the pH to 7.0, and sterilize at 121°C for 20 minutes.

[0035] Preparation of fermentation medium: use starch wastewater from potato starch factory as raw material, add soybean meal powder 10-30g / L, adjust pH value to 6.0-8.0, and sterilize at 121°C for 20min.

[0036] (2) Bacillus licheniformis (Bacillus licheniformis) W205 obtained in Example 1 was inoculated on the LB medium, and cultured in a constant temperature shaker at 37° C. at 180 r / min for 24 hours to prepare liquid seeds of Bacillus licheniformis;

[0037] (3) Inoculate the Bacillus subtilis (Bacillus subtilis) W309 obtained in Example 1 on the LB medium, in a constant temperature shaker at 37° C., vibrate at 180 r / min for 24 hours, and make Bacillus subtilis liquid seeds;

[0038] (4) Inoculate the Bacillus subtilis liquid seed in the fermentati...

Embodiment 3

[0039] Application of Example 3 Microbial Liquid Fertilizer in Tea Production

[0040] The test was carried out in Jiaotan Tea Farm (about 1000m above sea level) in Shanghang County, Longyan City, Fujian Province. The tea tree variety was Tieguanyin, and the tree was 12 years old. The planting method is border planting and dense planting, and the planting density is 3000-4000 plants / mu. The physical and chemical index parameters of the initial soil of the tea garden are as follows: pH is 5.8, total nitrogen is 0.203%, total phosphorus is 0.026%, total potassium is 1.12%, alkaline hydrolyzed nitrogen is 138.1mg / kg, available phosphorus is 106.7mg / kg, available potassium is 297mg / kg, Organic matter 45.0g / kg, humic acid 17.1g / kg.

[0041] The experiment was divided into two groups: blank control group (CK group) and test group (DKYF group).

[0042] Wherein blank control group (CK group) uses clear water, application rate: 1500L / mu;

[0043] The test group (DKYF group) applied...

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Abstract

The invention discloses microorganism liquid fertilizer containing bacillus. The liquid fertilizer is fermented liquid containing the bacillus with the viable count of 2.0*10<8> cfu / ml to 2.5*10<8> cfu / ml; the bacillus is mixed bacteria of bacillus licheniformis and bacillus subtilis; the bacillus licheniformis is bacillus licheniformis W205, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on August 8, 2016, the preservation number is as follows: CGMCC No. 12817, the address of the preservation unit is Microbiology Research Institute of Chinese Academy of Sciences, #3, No. 1 Yard, West Beichen Road, Chaoyang District, Beijing, and the postcode is 100101; the bacillus subtilis is bacillus subtilis W309, the strain is preserved in CGMCC on August 8,2016, the preservation number is as follows: CGMCC No. 12815, the address of the preservation unit is Microbiology Research Institute of Chinese Academy of Sciences, #3, No. 1 Yard, West Beichen Road, Chaoyang District, Beijing, and the postcode is 100101. Meanwhile, the invention further discloses a preparation method of the microorganism liquid fertilizer and application of the microorganism liquid fertilizer to planting of tea trees. The microorganism liquid fertilizer provided by the invention has the advantages of simple preparation method, low cost and simplicity in utilization; environment pollution is not caused and the yield, mouthfeel and quality of tea leaves can be remarkably improved; the microorganism liquid fertilizer has great significance of promoting production of the tea leaves and sustainable development of ecological tea gardens.

Description

technical field [0001] The invention relates to the technical field of agricultural fertilizers, in particular to a microbial liquid fertilizer containing bacillus and its preparation method and application. Background technique [0002] As a major tea-producing country in the world, China's domestic tea consumption has increased rapidly in recent years, reaching 1.6132 million tons in 2013, an increase of 225% over 2001. During the same period, global tea consumption increased from 2.9858 million tons in 2001 to 4.821 million tons in 2013. The large demand for tea in the market has directly promoted the large-scale planting of tea and the improvement of its quality. At present, China has become the fastest growing tea market with the greatest potential in the world. Improving tea quality and increasing tea output are two key technical issues for enhancing the market competitiveness of tea products and improving the economic benefits of tea production enterprises. Fertili...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/10C12R1/125
CPCC12N1/20
Inventor 林斌黄菊青徐庆贤郑怡钱蕾官雪芳张臣心
Owner INST OF AGRI ENG TECH FUJIAN ACAD OF AGRI SCI
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