Microorganism passivation method for remedying heavy metal lead polluted soil
A lead-contaminated soil and microorganism technology, applied in the restoration of contaminated soil, etc., can solve the problems of exogenous microorganism colonization, affecting the soil passivation effect, exogenous microorganisms are easily swallowed by other microorganisms, etc., to improve the colonization ability , improve properties, enhance the effect of stress resistance
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Embodiment 1
[0020] Embodiment 1 takes a certain polluted soil 1 in the northern suburb of Xuzhou City as an example to specifically illustrate the treatment steps and effects of the method of the present invention.
[0021] The specific steps of the method for repairing heavy metal lead-contaminated soil of the present embodiment are as follows:
[0022] (1) adding trehalose to the culture medium to carry out fermentation and cultivation of Bacillus subtilis, adding the bacterial solution obtained from fermentation to vinegar residue to prepare a microorganism / vinegar residue mixture;
[0023] a. Strain activation: Inoculate Bacillus subtilis on solid plate medium and culture at 30-37°C for 12-24 hours; solid plate medium: tryptone 10g / L; yeast extract 5g / L; sodium chloride 10g / L; agar 15-20g / L; pH natural.
[0024] b. Seed solution: Bacillus subtilis was inoculated from the solid plate culture medium into LB shaker flasks, and cultured at 30-37° C. for 12-24 hours to prepare the seed so...
Embodiment 2
[0034] Embodiment 2 takes wheat field soil 2 near a leather factory in Xuzhou as an example, and specifically illustrates the processing steps and effects of the method of the present invention.
[0035] The specific steps of the method for repairing heavy metal lead-contaminated soil of the present embodiment are as follows:
[0036] (1) adding trehalose to the culture medium to carry out fermentation and cultivation of Bacillus subtilis, adding the bacterial solution obtained from fermentation to vinegar residue to prepare a microorganism / vinegar residue mixture;
[0037] a. Strain activation: Inoculate Bacillus subtilis on solid plate medium and culture at 30-37°C for 12-24 hours; solid plate medium: tryptone 10g / L; yeast extract 5g / L; sodium chloride 10g / L; agar 15-20g / L; pH natural.
[0038] b. Seed solution: Bacillus subtilis was inoculated from the solid plate culture medium into LB shaker flasks, and cultured at 30-37° C. for 12-24 hours to prepare the seed solution. ...
Embodiment 3
[0047] Embodiment 3 takes the soil 3 near a glass factory in Xuzhou as an example to specifically illustrate the treatment steps and effects of the method of the present invention.
[0048] The specific steps of the method for repairing heavy metal lead-contaminated soil of the present embodiment are as follows:
[0049] (1) adding trehalose to the culture medium to carry out fermentation and cultivation of Bacillus subtilis, adding the bacterial solution obtained from fermentation to vinegar residue to prepare a microorganism / vinegar residue mixture;
[0050] a. Strain activation: Inoculate radiation-tolerant black yeast F134 on YPD plate medium, and culture at 25-30°C for 5-7 days; YPD plate medium: peptone 20g / L; yeast extract 10g / L; glucose 20g / L; agar powder 16g / L; pH natural.
[0051] b. Seed solution: Inoculate the black yeast F134 from the YPD plate medium into the MEA shake flask, and culture it at 25-30° C. for 48-72 hours to prepare the seed solution. MEA medium: ...
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