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Identification and application of biocontrol bacillus strain

A bacillus and biocontrol technology, applied in the field of agricultural microorganisms, can solve problems such as human health hazards, soil hardening and acidification, and aggravate the risk of chemical pesticide dosage, and achieve good control effects and disease reduction effects.

Inactive Publication Date: 2018-10-12
十堰市农业科学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the perennial use of chemical agents, the disadvantages and problems are becoming more and more prominent. The emergence of pathogenic bacteria resistance not only further aggravates the risk of chemical pesticide use, but also causes environmental pollution and soil compaction and acidification, and the harm to human health has greatly exceeded expectations.

Method used

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  • Identification and application of biocontrol bacillus strain
  • Identification and application of biocontrol bacillus strain
  • Identification and application of biocontrol bacillus strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Isolation of bacterial strains and detection of antagonistic effects

[0031] Collect 10g of healthy wheat rhizosphere soil in Yuejiachuan Village, Liuliping Town, Shiyan City, grind it with a sterilized mortar, put it into a triangular flask filled with 90ml of sterile deionized water, shake and suspend it for 10min, and draw 1ml from the triangular flask Put the suspension into a sterilized tube filled with 9ml sterile deionized water and continue to shake and suspend for 5min, then further dilute the suspension according to a 10-fold gradient to a concentration of 10 -4 、10 -5 、10 -6 three concentrations. The suspensions of these three concentrations were placed in an oven at 80°C for 15 minutes to select bacteria that could produce spores.

[0032] Take 0.1ml of the liquids of the three concentrations and put them on the 1 / 10TSA plate added with cycloacetimide (50μg / ml) and spread evenly with a coating stick, and apply three plates for each concentrati...

Embodiment 2

[0033] Example 2: Rapid identification of 16srDNA, gryA and rpoB of bacterial strain YJC-9

[0034] Recent studies have found that the gene similarity of Bacillus is very high, these species include Bacillus subtilis (Bacillus subtilis, Cohn, 1872), Bacillus amyloliquefaciens (Priest et al., 1987), Bacillus atrophaeus ( Bacillus atrophaeus, Nakamura, 1989), Bacillus mojavensis, Roberts et al., 1994), Bacillus sonorensis (Palmisano et al., 2001), Bacillus tequilensis, Gatson et al., 2006 and methylotrophic Bacillus (Bacillus vallismortisRoberts et al., 1996) and Bacillus velezensis et al., 2005a), etc., the 16srDNA similarity of these bacillus is above 99%. Studies have shown that it is particularly important to use multi-locus sequence analysis methods to detect and identify bacterial species. gryA is the fastest-evolving gene in the phylogenetic evolution of Bacillus, and rpoB gene is also widely used in the identification and classification of Bacillus populations. Therefo...

Embodiment 3

[0037] Example 3: Detection of YJC-9's inhibitory effect on other plant pathogens

[0038] The laboratory will identify preserved plant pathogenic fungi Rhizoctonia cerealis, Fusarium graminearum, Sclerotinia sclerotiorum, Botrytis cinerea, pepper root rot After Fusarium solani, Fusarium oxysporum, and Alternaria alternata were reactivated, holes were punched with a 5mm sterile puncher, and the fungus cakes were inoculated in the center of the PDA medium, 3cm away from the fungus cakes. YJC-9 strains were inoculated at the place, and the PDA medium only inoculated with pathogenic bacteria was used as a control. After 7 days, the antibacterial bandwidth of YJC-9 antagonistic bacteria to these several pathogenic bacteria was recorded, and the growth inhibition rate was calculated. The results are shown in Table 1 and Figure 5 .

[0039] The inhibitory effect of table 1YJC-9 on pathogenic fungi

[0040]

[0041]

[0042] It can be seen from Table 1 that B. velezensis YJC...

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Abstract

The invention discloses identification and application of a biocontrol bacillus strain, and belongs to the technical field of agricultural microorganisms. Biocontrol bacillus is bacillus velezensis YJC-9, the strain is already reserved in The China Typical Culture Collection Center of Wuhan university at 11thApril in 2018, and the preservation serial number of the strain is CCTCC NO.M 2018196. Thebiocontrol bacillus strain can prevent and control wheat sharp eyespot, wheat scab, sclerotinia rot of colza, gray mold of strawberries, pepper root rot, kidney bean blight and tobacco alternariaalternata, is a biocontrol bacterium having broad-spectrum resistance, and has good application prospects in agricultural disease prevention and control.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and mainly relates to a strain of bacillus velei and its rapid identification method and its application in preventing and treating wheat sheath blight and the like. Background technique [0002] Wheat is the main food crop in my country, with an annual planting area of ​​300-400 million mu and a total output of more than 100 million tons, ranking first in the world. In recent years, with the continuous expansion of wheat area, the globalization of commodities, the slowdown of variety renewal, the promotion of agricultural farming methods and high-yield cultivation techniques, etc., the incidence of wheat sheath blight has become increasingly serious in my country's wheat planting areas, especially in major wheat producing areas. The North China Plain, the Yangtze River Basin, Sichuan-Guizhou and other places occur frequently and tend to increase year by year. In severe cases, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12Q1/689A01N63/00A01P3/00C12R1/07
CPCA01N63/00C12Q1/689C12N1/205C12R2001/07
Inventor 张凡蔡高磊江爱明柯尊伟赵昌松王锋尖胡学明彭敏彭宣和刘慧鸣欧阳友香
Owner 十堰市农业科学院
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