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Dual-valve multi-stimulation-responsive drug carrier constructed on basis of mesoporous silicon/cyclodextrin/zinc oxide quantum dots and preparation method thereof

A zinc oxide and mesoporous silicon technology is applied in the field of "dual valve" multi-stimulus-responsive drug carrier and its preparation, and achieves the effects of high efficiency, reducing premature release and improving drug utilization rate

Active Publication Date: 2018-09-21
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The research on cyclodextrin / mesoporous silicon drug carrier has made some progress in drug controlled release, but there are still many problems. The cyclodextrin / mesoporous silicon drug carrier system is in the research and development stage, and most of the research is material biocompatibility. and degradation performance and drug release rate, there is a lack of research on the simultaneous construction of drug carriers by cyclodextrin anhydride and other nano-valve

Method used

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  • Dual-valve multi-stimulation-responsive drug carrier constructed on basis of mesoporous silicon/cyclodextrin/zinc oxide quantum dots and preparation method thereof
  • Dual-valve multi-stimulation-responsive drug carrier constructed on basis of mesoporous silicon/cyclodextrin/zinc oxide quantum dots and preparation method thereof
  • Dual-valve multi-stimulation-responsive drug carrier constructed on basis of mesoporous silicon/cyclodextrin/zinc oxide quantum dots and preparation method thereof

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preparation example Construction

[0041] The preparation steps of MSN are as follows: first dissolve NaOH (0.28g) and CTAB (1g) in 480mL of secondary water and raise the temperature to 80°C, then slowly add TEOS (5mL) into the system dropwise, and continue stirring for 2h to produce white precipitate. After the reaction, the product was centrifuged (9500r / min×10min), washed with secondary water and methanol several times, and vacuum-dried at 50°C to obtain the MSN containing the template CTAB (CTAB@MSN). In order to remove the template CTAB, the obtained CTAB@MSN (0.5g) needs to be refluxed in methanol (160mL) containing 9mL of concentrated hydrochloric acid for 48h, then washed and centrifuged several times with secondary water and methanol, and dried under vacuum at 50°C to obtain MSN .

[0042] Such as figure 2 As shown in the small-angle X-ray diffraction pattern, it can be seen that the MSN channels are arranged in a hexagonal shape, with three characteristic small-angle diffraction peaks corresponding...

Embodiment 1

[0054] A composite nano drug carrier system, comprising the steps of:

[0055] 1. Preparation of aminated zinc oxide quantum dots (ZnO-NH 2 QDs)

[0056] Disperse ZnO QDs in 20ml of anhydrous N'N-dimethylformamide (DMF), add 100ul of triaminopropyltriethoxysilane (APTES), react at 120°C for 20min, and then wash with DMF for several minutes times, vacuum drying at 50 degrees to obtain white ZnO-NH 2 QDs.

[0057] 2. Preparation of drug carrier DOX@MSN-N-ZnO blocked by zinc oxide quantum dots

[0058] Disperse 0.2g of MSN-COOH in 50ml of dichloromethane, add 50mg of doxorubicin (DOX), stir at room temperature for 24 hours in the dark, add 8.4g of DCC, 0.64g of DMAP after activation at room temperature for 8 hours, add 0.5g of ZnO -NH 2 After reacting for 48 hours, the QDs were washed twice with methanol and twice with water, and freeze-dried to obtain DOX@MSN-N-ZnO.

[0059] 3. Preparation of anhydrided cyclodextrin β-CD-cit

[0060] Take 1gβ-CD-NH 2 , dissolved in 100ml...

Embodiment 2

[0074] A composite nano drug carrier system, comprising the steps of:

[0075] 1. Preparation of aminated zinc oxide quantum dots (ZnO-NH 2 QDs)

[0076] Disperse ZnO QDs in 20ml of anhydrous N'N-dimethylformamide (DMF), add 100ul of triaminopropyltriethoxysilane (APTES), react at 120°C for 20min, and then wash with DMF for several minutes times, vacuum drying at 50 degrees to obtain white ZnO-NH 2 QDs.

[0077] 2. Preparation of drug carrier DOX@MSN-N-ZnO blocked by zinc oxide quantum dots

[0078] Disperse 0.2g of MSN-COOH in 50ml of dichloromethane, add 100mg of doxorubicin (DOX), stir at room temperature for 24 hours in the dark, add 8.4g of DCC, 0.64g of DMAP after activation at room temperature for 8 hours, add 0.5g of ZnO -NH 2 After reacting for 48 hours, the QDs were washed twice with methanol and twice with water, and freeze-dried to obtain DOX@MSN-N-ZnO.

[0079] 3. Preparation of anhydrided cyclodextrin β-CD-cit

[0080] Take 1gβ-CD-NH 2 , dissolved in 100m...

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Abstract

The invention discloses a dual-valve multi-stimulation-responsive drug carrier constructed on the basis of mesoporous silicon / cyclodextrin / zinc oxide quantum dots and a preparation method thereof. With mesoporous silicon nanoparticles as a carrier, after drug loading, blocking is performed by using aminated zinc oxide quantum dots to obtain a drug carrier blocked by the zinc oxide quantum dots; then, after the drug carrier blocked by the zinc oxide quantum dots is connected with ferrocenecarboxylic acid through chemical bonds, the drug carrier further reacts with anhydrided cyclodextrin for secondary blocking to obtain a composite nanometer drug carrier system. The composite nanometer drug carrier system has the properties of dual pH sensitivity, H2O2 oxidation sensitivity and fluorescenceimaging, has more efficient targeting drug administering ability in cancer cells, and can effectively increase the drug utilization rate.

Description

technical field [0001] The invention belongs to the field of drug carriers, and specifically relates to a "double valve" multi-stimuli-responsive drug carrier based on mesoporous silicon / cyclodextrin / zinc oxide quantum dots and a preparation method thereof. Background technique [0002] In cancer therapy, there has been strong interest in stimuli-responsive drug carriers to improve therapeutic efficacy and reduce drug side effects. Compared with polymer nanoparticles, micelles, and liposomes, mesoporous silica nanoparticles (MSNs) have the advantages of high surface area, large pore volume, adjustable pore size, good biocompatibility, and easy functionalization. It has become a promising drug delivery vehicle. Based on MCM-41-type mesoporous silicon (MSNs), a series of stimuli such as pH, redox, light, and enzymes have been developed through various "valves" including inorganic nanoparticles, organic molecules, and biomolecules. A variety of stimuli-responsive drug carrier...

Claims

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Application Information

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IPC IPC(8): A61K47/04A61K47/52A61K47/54A61K47/69A61K49/00A61K31/704A61P35/00
CPCA61K31/704A61K47/02A61K49/0019A61K47/52A61K47/54A61K47/6951A61P35/00
Inventor 李草陈辉江兵兵李爽赵童童
Owner HUBEI UNIV
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