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Method for using two-dimensional liquid chromatogram to detect compound nanxing acesodyne plaster

A two-dimensional liquid chromatography and detection method technology, which is applied in the field of two-dimensional liquid chromatography detection of Compound Nanxing Zhitong Ointment, can solve the problems of cumbersome pretreatment methods and complex components of Compound Nanxing Analgesic Ointment, and achieve simplified sample pretreatment methods, The effect of improving the quality control level and stabilizing the measurement results

Active Publication Date: 2018-09-18
JIANGSU KANION PHARMA CO LTD +1
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Problems solved by technology

Since the composition of Compound Nanxing Zhitong Ointment is extremely complex, and contains auxiliary materials such as vaseline and liquid paraffin, the pretreatment method of the sample is relatively cumbersome. The current quality standard of this preparation only uses the HPLC method to determine the content of hypoaconitine.

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  • Method for using two-dimensional liquid chromatogram to detect compound nanxing acesodyne plaster
  • Method for using two-dimensional liquid chromatogram to detect compound nanxing acesodyne plaster
  • Method for using two-dimensional liquid chromatogram to detect compound nanxing acesodyne plaster

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Embodiment Construction

[0039] As mentioned above, the present invention aims to provide a method for detecting diester alkaloids in Compound Nanxing Zhitong Ointment.

[0040]Experimental equipment: Agilent 1290 Infinity II two-dimensional liquid chromatography system (including 1290 Infinity II high-speed pump, 1290 Infinity II autosampler, 1290 Infinity II column thermostat, 1290 Infinity II diode array detector, 1290 Infinity II binary pump for second-dimensional analysis, 1290 Infinity II diode array Detector, used for second-dimensional analysis, external valve drive), BUCHI R-3 rotary evaporator, KH-300DB numerical control ultrasonic cleaner, ME303E electronic balance, XS205DU electronic analytical balance.

[0041] Reagents and test drugs: Hypoaconitine (National Institute for Food and Drug Control, batch number 110798-201609), aconitine (National Institute for Food and Drug Control, batch number: 110720-201111), neoaconitine (National Institute for Food and Drug Control Research Institute, b...

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Abstract

The invention discloses a detection method of compound nanxing acesodyne plaster diester alkaloids. The detection method comprises the steps that 1, a test sample solution is prepared, a compound nanxing acesodyne plaster sample is taken to be subjected to extraction and impurity removal, and the test sample solution is prepared; and 2, the test sample solution is sucked and subjected to measurement of ester alkaloids by adopting a two-dimensional liquid chromatogram method. The precision and the stability of the method provided by the invention are both relatively good, the separation level of to-be-tested components in the compound nanxing acesodyne plaster is effectively improved, the detection sensibility is greatly enhanced, accurate quantitative analysis is conducted on three kinds of diester alkaloids in the preparation, the quality control level of the product is improved, and theoretical support is provided for clinical medication.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to a method for detecting compound Nanxing Zhitong Ointment by two-dimensional liquid chromatography. Background technique [0002] In recent years, with the gradual maturity of commercial two-dimensional liquid chromatography instrument technology, two-dimensional liquid chromatography combined with multiple separation modes, such as heart-cutting, multi-heart-cutting and comprehensive two-dimensional liquid chromatography, have appeared, which can be divided into There are two ways, online and offline, in which the online two-dimensional liquid chromatography can selectively transfer the one-dimensional fraction to the second-dimensional chromatographic column for analysis, which can well solve the problem of limited separation ability of conventional chromatography. [0003] Compound Nanxing Pain Relief Ointment is composed of 12 herbs, including Aracnata si...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/46G01N30/34
CPCG01N30/02G01N30/06G01N30/34G01N30/46
Inventor 肖伟马阳李郭帅耿婷黄文哲王振中曹亮
Owner JIANGSU KANION PHARMA CO LTD
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