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Non-toxic photoelectrochemistry competition immunoassay method of zearalenone based on peptide sensor

A zearalenone and photoelectrochemical technology, which is applied in the direction of material electrochemical variables, material analysis through electromagnetic means, and material analysis, can solve problems such as toxicity and hazards, and achieve the effect of enhancing transmission speed and improving photoelectric conversion efficiency

Inactive Publication Date: 2018-09-14
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the traditional detection method of zearalenone, the antigen is mainly the standard solution of zearalenone, which will cause harm to the human body due to its toxicity

Method used

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  • Non-toxic photoelectrochemistry competition immunoassay method of zearalenone based on peptide sensor
  • Non-toxic photoelectrochemistry competition immunoassay method of zearalenone based on peptide sensor
  • Non-toxic photoelectrochemistry competition immunoassay method of zearalenone based on peptide sensor

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Experimental program
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Effect test

Embodiment 1

[0029] A non-toxic photoelectrochemical competitive immunoassay for zearalenone based on a peptide sensor (e.g. figure 1 shown):

[0030] (1) Pretreatment of the glassy carbon electrode: the glassy carbon electrode is first mechanically polished and polished on the suede covered with alumina powder, washed with secondary water to remove the residual powder on the surface, and then moved into an ultrasonic water bath for cleaning until it is cleaned, and finally Wash thoroughly with ethanol, dilute acid and water;

[0031] (2) Drop 3 μL of CNHs suspension with a concentration of 3 mg / mL on the surface of a clean glassy carbon electrode, dry it under infrared light, and cool to room temperature;

[0032] (3) React the mercaptoethylamine (MEA) solution with the gold cone (Au NCs) solution to obtain the MEA / Au NCs composite solution, then apply 3 μL of the Au NCs / MEA composite solution onto the surface of the modified electrode and dry it in an oven dried, then cooled at room te...

Embodiment 2

[0037] A nontoxic photoelectrochemical competitive immunoassay method for zearalenone based on a peptide sensor, the steps are as follows:

[0038] (1) The three-electrode system was used for measurement, the Poly(Gly) / Au NCs / CNHs / Ab / peptide modified electrode prepared in Example 1 was used as the working electrode, Ag / AgCl was used as the reference electrode, and the platinum wire electrode was used as the counter electrode , using a photoelectrochemical workstation to detect, set the voltage to 0.1V, switch the lamp every 10s, and the monochromatic light emitted by the xenon lamp to excite the light source is filtered by the monochromator before use;

[0039] (2) In PBS buffer solution with pH 7.0, detect 1×10 by photoelectrochemical workstation -6 ng / mL–1 ng / mL series of zearalenone standard solutions with different concentrations, by recording the different current signals before and after switching the lamp, draw the working curve. Figure 2 A is different concentrations...

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Abstract

The invention discloses a non-toxic photoelectrochemistry competition immunoassay method of zearalenone based on a peptide sensor. According to a sensing interface building method, carbon nanohorns, cysteamine functional golden cone and polyglycine are used as substrates; corn zeranol antibodies (Ab) are further immobilized; tyramine functionalized nanometer rutile type TiO2 mesoscopic crystals are used for immobilizing peptide chains with the specific sequences as a photoelectric probe; the peptide chain has the effect of simulating the zearalenone, the zearalenone can compete with the markedphotoelectric probe to combine the corn zeranol antibodies immobilized on the sensing interface. The photoelectric probe combined onto the sensing interface can further combine with tyramine-Rutile TiO2 mesoscopic crystal compounds under the catalysis effect of horseradish peroxidase; optoelectronic signals can be further amplified. On the basis, the non-toxic photoelectrochemistry competition immunoassay method on the corn zeranol can be built.

Description

technical field [0001] The invention belongs to the technical field of novel functional materials and biosensing detection, and specifically relates to a nontoxic photoelectrochemical competitive immunoassay method for zearalenone based on a peptide sensor. Background technique [0002] Photoelectrochemical (PEC) detection uses light as the excitation signal and photocurrent as the detection signal. By using different forms of energy as the excitation signal and detection signal, the excitation and detection signals do not interfere with each other, so the background signal is low and can be obtain higher sensitivity. In the construction process of photoelectrochemical sensors, the choice of photosensitive materials is crucial to the response of the signal. Among the currently used materials, TiO 2 Nanomaterials are ideal materials for photocatalytic and photoelectrochemical sensors because of their unique photocatalytic activity, non-toxicity, and excellent chemical and ph...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/30G01N33/569G01N33/64
CPCG01N27/305G01N27/308G01N27/3278G01N33/56961G01N33/64G01N2333/37G01N2333/415
Inventor 戴宏陈妍洁陈四红高利红衣欢郑祥钦颜建英林丹玫
Owner FUJIAN NORMAL UNIV
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