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Terphenyl compound as well as preparation method and application thereof

A technology of terphenyls and compounds, which is applied in the field of microbial engineering, can solve the problems of underutilization, etc., and achieve the effect of short cycle and simple process

Inactive Publication Date: 2018-09-04
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Most of the terphenyl compounds so far have been isolated from fungi, and less than 10 terphenyl compounds have been isolated from Streptomyces, so Streptomyces is a good underutilized resource for the discovery of new terphenyls. source

Method used

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  • Terphenyl compound as well as preparation method and application thereof
  • Terphenyl compound as well as preparation method and application thereof
  • Terphenyl compound as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment approach

[0039] (1) The Streptomyces chrysalis 1510-2 described in the present invention is isolated from the body of the chrysalis chrysalis through strict surface disinfection.

[0040] (2) Streptomyces chrysalis 1510-2 of the present invention is in the form of air silk powder on the colonies of Gao's No. 1 solid medium, taupe-gray. It was initially identified as Streptomyces.

[0041](3) Streptomyces chrysalis 1510-2 of the present invention, on Gao's No. 1 agar medium, the air filaments are off-white, the base filaments are yellowish brown, and the spore filaments are long and straight, forming clusters. The spores are nearly spherical to ellipsoid, with a smooth surface. identified as Streptomyces.

[0042] (4) Streptomyces chrysalis 1510-2 described in the present invention has been preserved in the China Type Culture Collection Center (Address: Wuhan Luojiashan Wuhan University) on March 11, 2018, and the preservation number is CCTCC NO :M 2018111. .

Embodiment 1

[0044] Isolation and Purification of Streptomyces 1510-2

[0045] The Streptomyces used in the present invention is a strain of symbiotic actinomycetes isolated from the body of Sclerex chinensis from the suburbs of Dezhou City, Shandong Province in 2015, and the strain number is N1510-2.

[0046] Under aseptic conditions, the body surface of the yellow stalk wall wasp was sterilized with 70% alcohol, the bee was placed in a sterile mortar and a small amount of sterile water was added for grinding, and the grinding solution was diluted to 10- 1, 10-2, 10-3, respectively take 100 microliters of each gradient dilution solution in Gaucher’s medium and YMS medium, and place them in a 37°C incubator for cultivation. After the colonies grow, pick the spores for purification and culture , to obtain endosymbiontic actinomycetes of Sclerex chinensis.

Embodiment 2

[0047] Example 2: Identification of Streptomyces 1510-2

[0048] (1) Morphological identification:

[0049] The surface of the colony of Streptomyces 1510-2 on Gao's No. 1 medium is off-white, and the colony is round like figure 1 shown. Optical microscope inspection showed that the spore filaments were long and straight, and clustered. The spores are nearly spherical to oval, with a smooth surface, and were initially identified as Streptomyces such as figure 2 shown.

[0050] (2) Molecular identification: DNA was extracted from the mycelium according to the instructions of the Sangong Bacteria Genomic DNA Rapid Extraction Kit, and the forward primer was Fd2: 5'-GAGTTTGATCATGGCTCAG-3, and the reverse primer 16Sr: 5'-TTGCGGGACTTAACCCAACAT-3 16S rDNA sequence was amplified by PCR, and the PCR product was purified and sent to Huada Company for sequencing. The sequencing results were compared and analyzed by BLAST, similar sequences were selected, and the phylogenetic tree wa...

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Abstract

The invention belongs to the technical field of microorganisms and in particular relates to a terphenyl compound as well as a preparation method and application thereof. A terphenyl compound 2,5-diphenyl-3-methoxy-6-hydroxy-6-acetonyl cyclohexdienone is extracted from an extract of sceliphron madraspatanum symbiotic actinomycetes, the sceliphron madraspatanum symbiotic actinomycetes belong to edophytic steptomyces, and the 2,5-diphenyl-3-methoxy-6-hydroxy-6-acetonyl cyclohexdienone has a certain inhibition function on tumor cells, so that the 2,5-diphenyl-3-methoxy-6-hydroxy-6-acetonyl cyclohexdienone can be used as application of anti-tumor medicine lead compound. The 2,5-diphenyl-3-methoxy-6-hydroxy-6-acetonyl cyclohexdienone hs an activity inhibition function upon osteosarcoma cells U2OS, osteosarcoma Saos2, mucoepidermoid carcinoma cells and vinblastine-resistant cells.

Description

technical field [0001] The invention relates to the technical field of microbial engineering, in particular to a novel terphenyl compound 2,5-diphenyl-3-methoxy-6-hydroxyl-6-acetonyl extracted from the fermentation broth of an actinomycete Cyclohexadienone and its preparation and application. Background technique [0002] In nature, p-terphenyls (p-Terphenyls) are relatively rare natural products, and p-terphenyls are an important natural product mainly distributed in lichens and fungi. A variety of biological activities, including inhibition of tumor cell proliferation, specific inhibition of 5-lipoxygenase, anti-bacterial, anti-oxidation, etc. [0003] Most of the terphenyl compounds so far have been isolated from fungi, and less than 10 terphenyl compounds have been isolated from Streptomyces, so Streptomyces is a good underutilized resource for the discovery of new terphenyls. source. [0004] Symbiotic actinomycetes exist in plants and animals. At present, most of th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/26C07C49/753A61K31/122A61P35/00C12R1/465
CPCA61K31/122A61P35/00C07C49/753C12N1/20C12P7/26C07C2601/16C12N1/205C12R2001/465
Inventor 谢卫东宋亚洁彭爱红王哲李霞
Owner SHANDONG UNIV
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