Vascularization-promoting bone-tissue engineering stent and preparation method and application thereof

Abstract

The invention provides a preparation method of a vascularization-promoting bone-tissue engineering stent. The method comprises the step that a chemical sedimentation method is adopted to react water soluble calcium salt, water soluble copper salt, water soluble zinc salt and the aqueous solution of a surface active agent with the aqueous solution of phosphate to prepare calcium-deficient apatite slurry, and powder body is obtained after drying is conducted, wherein the mole ratio of the water soluble calcium salt to the water soluble copper salt and the water soluble zinc salt is 15-168:1:1; the obtained powder body is mixed with a liquid-phase solution to be prepared into slurry and injected into a mold, and a porous calcium orthophosphate ceramic stent is obtained after drying shaping and high-temperature sintering are conducted; microspheres carrying GDF-5 are loaded on the surface of the porous calcium orthophosphate ceramic stent, and the vascularization-promoting bone-tissue engineering stent is obtained after vacuum drying is conducted. The tissue engineering stent has good osteoconductivity, osteoinductive, biological degradation performance and vascularization promoting performance, the difficult problem of the rapid repairing of bone defect is solved, and the method has good actual application prospects.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and relates to the technical field of biomedical scaffold materials suitable for human hard tissue repair, in particular to a scaffold for promoting vascularization of bone tissue engineering and its preparation method and application. Background technique [0002] With the development of society and economy and the explosive growth of vehicles, bone defects caused by trauma, infection, tumor and congenital diseases have caused people's demand for bone defect repair to grow rapidly. Bone tissue itself has the ability to regenerate, but when the size of the defect is too large, that is, a large bone defect occurs, the bone tissue cannot repair and rebuild itself, which requires the use of bone substitutes for repair. [0003] At present, the commonly used clinical methods for repairing large segmental bone defects are: (1) Autologous bone grafting: As the gold standard for repairing b...

AI Technical Summary

Problems solved by technology

However, compared with the single doping of metal ions, the development of multi-element doped calcium phosphate materials for bone tissue repair still faces important challenges: (1) the effect of metal ion co-doping on the physical and chemical properties of calcium phosphate; (2) Whether the release kinetics and release concentration of different metal ions in co-doped calcium phosphate materials reach the effective therapeutic concentration respectively; (3) Whether there is a synergistic effect between metal ions on the expression of biologically active factors and cell behavior and the specific mechanism remains to be explored

In view of the fact that the existing research still cannot solve the above problems, it is still difficult for people to obtain materials that can rapidly promote bone repair by preparing biomaterials loaded with related factors or metal ions.

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