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Nanometer bionic scaffold material and preparation method thereof

A nano-bionic and bone material technology, applied in prosthetics, medical science, etc., can solve problems such as single direction and inability to obtain high bionics

Inactive Publication Date: 2010-01-20
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A molecular template can only provide one kind of nucleation site and one active center. The growth of crystals on such a molecular template leads to a single direction and a relatively simple structure level, and it is impossible to obtain materials with highly biomimetic multi-level excellent structures.

Method used

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  • Nanometer bionic scaffold material and preparation method thereof
  • Nanometer bionic scaffold material and preparation method thereof
  • Nanometer bionic scaffold material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Weigh 0.5g of type I collagen (collagen I, COL I) and dissolve it in 0.5M acetic acid solution to prepare 100ml of collagen solution with a concentration of 5mg / ml. In order to maintain the activity of collagen, the whole reaction process is carried out at 4°C in progress.

[0028] 2) Weigh 0.5 g of bovine serum albumin (BSA) and dissolve it in deionized water to prepare 100 ml of serum protein solution with a concentration of 5 mg / ml. The purity of serum protein has a great influence on the experiment, so it is necessary to use high purity The serum protein used in this example is bovine serum albumin (cell culture grade).

[0029] 3) Prepare 200 ml of 0.1 mol / L calcium nitrate aqueous solution and 0.06 mol / L diammonium hydrogen phosphate respectively, the molar ratio of calcium to phosphorus is 5:3, and then filter them with 0.22 μm filter membrane once and save them for later use. This is to eliminate the influence of impurity components on template assembly and ...

Embodiment 2

[0036] 1) Weigh 0.5 g of silk fibroin (SF) lyophilized powder and dissolve it in a ternary solvent prepared by calcium chloride, absolute ethanol and deionized water (calcium chloride: ethanol: water = 1: 2: 8), then dialyze the calcium alcohol solution of silk fibroin with a molecular weight cut-off of 12,000, and finally obtain 100 ml of pure silk fibroin solution with a concentration of 5 mg / ml.

[0037] 2) Weigh 0.5g of type II collagen (collagen, COL II) and dissolve it in 0.5M acetic acid solution to prepare 100ml of collagen solution with a concentration of 5mg / ml. In order to maintain the activity of type II collagen, the entire reaction process is carried out at 4°C environment.

[0038] 3) Prepare 200 ml of 0.1 mol / L calcium acetate monohydrate solution and 0.06 mol / L disodium hydrogen phosphate respectively, the molar ratio of calcium to phosphorus is 5:3, and then filter them with 0.22 μm filter membrane once and save them for later use. This is to eliminate the i...

Embodiment 3

[0045] 1) Weigh 0.5g of type V collagen (collagen V, COL I) and dissolve it in 0.5M acetic acid solution to prepare 100ml of collagen solution with a concentration of 5mg / ml. In order to maintain the activity of collagen, the entire reaction process is carried out at 4°C in progress.

[0046] 2) Weigh 0.5 g of hyaluronic acid (hyaluronic acid, HA, molecular weight is 5KD) and dissolve it in deionized water to prepare 100 ml of hyaluronic acid solution with a concentration of 5 mg / ml. The purity of hyaluronic acid has a great influence on the experiment. Therefore, hyaluronic acid with high purity should be selected, and hyaluronic acid (cell culture grade) was used in this embodiment.

[0047] 3) Prepare 200 ml of 0.1 mol / L calcium nitrate aqueous solution and 0.06 mol / L ammonium dihydrogen phosphate respectively, the molar ratio of calcium to phosphorus is 5:3, and then filter them with 0.22 μm filter membrane once and save them for later use. This is to eliminate the influe...

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Abstract

The invention specifically relates to a nanometer bionic scaffold material and a preparation method thereof. The nanometer bionic scaffold material is assembled in synergy with a dimolecular template, wherein the dimolecular template comprises a protein and protein template, a protein and polyoses template, a polyoses and polyoses molecule template, a synthesis molecule and synthesis molecule template, a synthesis molecule template and a natural molecule template. The preparation method comprises the following steps: mixing one template with solution containing calcium ion; mixing the other template with solution containing phosphate radical ion; adding the solution containing phosphate radical ion into the solution containing calcium ion after evenly mixing the two plates; regulating thepH value of the mixed solution to be 7.4 and then transferring to the water at a constant temperature of 37 DEG C to obtain sediment, i.e, the nanometer bionic scaffold material. The bionic scaffold material not only has favorable biocompatibility and degradability, but also has bionic multi-layer assembling structure of highly-approximate natural scaffold, thereby being a bionic scaffold material in true sense and having wide application prospect in clinical scaffold repair.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a nano bionic bone material and a preparation method thereof. Background technique [0002] Natural bone is a biomineralized complex with a multi-level assembly structure and perfect performance. Its organic component is mainly type I collagen (collagen), and its inorganic component is mainly nano-hydroxyapatite (hydroxyapatite). Recent studies have shown that the formation of bone tissue mainly includes two processes: self-assembly of fibrous collagen and directional deposition of nano-hydroxyapatite, and these two processes are carried out simultaneously and interact. Therefore, the current template assembly method is the best strategy to obtain a high degree of biomimetic. However, the existing research is almost limited to the regulation and control of the nucleation of inorganic crystal nano-hydroxyapatite by a single template, and the formation of our natura...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/58A61L27/24A61L27/22A61L27/20A61L27/12
Inventor 张胜民王江林
Owner HUAZHONG UNIV OF SCI & TECH
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