A kind of swine fever live vaccine compound preparation and preparation method thereof
A technology of swine fever live vaccine and compound preparation, which is applied in the field of swine fever live vaccine compound preparation and its preparation, can solve the problems of maternal antibody interference, weak humoral immunity, and low antibody titer, and achieve stable traits and protection rate effects Good, the effect of breaking through the obstruction of maternal antibodies
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Embodiment 1
[0024] (1) Weigh 6g of hydrolyzed gelatin and 25g of skimmed milk powder, dissolve in a small amount of water for injection, and sterilize at 120°C for later use;
[0025] (2) Weigh 5g of hydrolyzed milk protein, 30g of sucrose, 9g of mannitol, 3g of sodium glutamate and 15g of trehalose, dissolve in a small amount of water for injection, filter through a filter membrane with a pore size of 0.22 μm, and set aside;
[0026] (3) Under sterile conditions, mix 80g of CSF splenocyte vaccine, 80ml of CSF ST cell vaccine, and 100ml of CSF bovine testicular cell vaccine;
[0027] (4) Mix the material liquid prepared in step (1) (2) (3), add water for injection to 1L, mix evenly, divide the package, and then place it in a freeze-drying box at 4°C at a rate of 1°C / min Cool down to -30°C, keep it for 3h, then turn on the vacuum pump, evacuate the inside of the box to 0.01mbar, then raise the temperature to -8°C within 16h, then raise the temperature to 23°C at a rate of 5°C / h, and keep i...
Embodiment 2
[0029] (1) Weigh 5g of hydrolyzed gelatin and 30g of skim milk powder, dissolve in a small amount of water for injection, and sterilize for later use;
[0030] (2) Weigh 6 g of hydrolyzed milk protein, 30 g of sucrose, 10.0 g of mannitol, 4 g of sodium glutamate, and 20 g of trehalose, dissolve in a small amount of water for injection, filter through a filter membrane with a pore size of 0.22 μm, and set aside;
[0031] (3) Under sterile conditions, mix 100g of CSF splenocyte vaccine, 120ml of CSF ST cell vaccine, and 150ml of CSF bovine testicular cell vaccine;
[0032] (4) Mix the material liquid prepared in step (1) (2) (3), add water for injection to 1L, mix evenly, divide the package, and then place it in a freeze-drying box at 4°C at a rate of 2°C / min Cool down to -30°C, keep it for 3h, then turn on the vacuum pump, evacuate the inside of the box to 0.01mbar, then raise the temperature to -6°C within 20h, then raise the temperature to 20°C at a rate of 5°C / h, keep it for...
Embodiment 3
[0034] (1) Weigh 5g of hydrolyzed gelatin and 20g of skimmed milk powder, dissolve in a small amount of water for injection, and sterilize for later use;
[0035] (2) Weigh 8 g of hydrolyzed milk protein, 20 g of sucrose, 9 g of mannitol, 3 g of sodium glutamate, and 10.0 g of trehalose, dissolve in a small amount of water for injection, filter through a filter membrane with a pore size of 0.22 μm, and set aside;
[0036] (3) Under sterile conditions, mix 100g of CSF splenocyte vaccine, 100ml of CSF ST cell vaccine, and 120ml of CSF bovine testicular cell vaccine;
[0037] (4) Mix the material liquid prepared in step (1) (2) (3), add water for injection to 1L, mix evenly, divide the package, and then place it in a freeze-drying box at 4°C at a rate of 2°C / min Cool down to -30°C, keep it for 3h, then turn on the vacuum pump, evacuate the inside of the box to 0.01mbar, then raise the temperature to -4°C within 22h, then raise the temperature to 22°C at a rate of 6°C / h, keep it f...
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