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Swine fever live vaccine compound preparation and preparation method thereof

A technology for live swine fever vaccine and compound preparation, which is applied in the field of compound preparation of live swine fever vaccine and its preparation, can solve the problems of maternal antibody interference, weak humoral immunity, low antibody titer, etc., and achieves the effect of stable traits and protection rate. Good, break through the effect of maternal antibodies

Active Publication Date: 2018-08-24
派生特(福州)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The object of the present invention is to provide a compound preparation of swine fever live vaccine and its preparation method, which solves the problems of long blank period after immunization of traditional swine fever vaccine, weak humoral immunity, low antibody titer and maternal antibody interference.

Method used

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  • Swine fever live vaccine compound preparation and preparation method thereof
  • Swine fever live vaccine compound preparation and preparation method thereof
  • Swine fever live vaccine compound preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Weigh 6g of hydrolyzed gelatin and 25g of skimmed milk powder, dissolve in a small amount of water for injection, and sterilize at 120°C for later use;

[0025] (2) Weigh 5g of hydrolyzed milk protein, 30g of sucrose, 9g of mannitol, 3g of sodium glutamate and 15g of trehalose, dissolve in a small amount of water for injection, filter through a filter membrane with a pore size of 0.22 μm, and set aside;

[0026] (3) Under sterile conditions, mix 80g of CSF splenocyte vaccine, 80ml of CSF ST cell vaccine, and 100ml of CSF bovine testicular cell vaccine;

[0027] (4) Mix the material liquid prepared in step (1) (2) (3), add water for injection to 1L, mix evenly, divide the package, and then place it in a freeze-drying box at 4°C at a rate of 1°C / min Cool down to -30°C, keep it for 3h, then turn on the vacuum pump, evacuate the inside of the box to 0.01mbar, then raise the temperature to -8°C within 16h, then raise the temperature to 23°C at a rate of 5°C / h, and keep i...

Embodiment 2

[0029] (1) Weigh 5g of hydrolyzed gelatin and 30g of skim milk powder, dissolve in a small amount of water for injection, and sterilize for later use;

[0030] (2) Weigh 6 g of hydrolyzed milk protein, 30 g of sucrose, 10.0 g of mannitol, 4 g of sodium glutamate, and 20 g of trehalose, dissolve in a small amount of water for injection, filter through a filter membrane with a pore size of 0.22 μm, and set aside;

[0031] (3) Under sterile conditions, mix 100g of CSF splenocyte vaccine, 120ml of CSF ST cell vaccine, and 150ml of CSF bovine testicular cell vaccine;

[0032] (4) Mix the material liquid prepared in step (1) (2) (3), add water for injection to 1L, mix evenly, divide the package, and then place it in a freeze-drying box at 4°C at a rate of 2°C / min Cool down to -30°C, keep it for 3h, then turn on the vacuum pump, evacuate the inside of the box to 0.01mbar, then raise the temperature to -6°C within 20h, then raise the temperature to 20°C at a rate of 5°C / h, keep it for...

Embodiment 3

[0034] (1) Weigh 5g of hydrolyzed gelatin and 20g of skimmed milk powder, dissolve in a small amount of water for injection, and sterilize for later use;

[0035] (2) Weigh 8 g of hydrolyzed milk protein, 20 g of sucrose, 9 g of mannitol, 3 g of sodium glutamate, and 10.0 g of trehalose, dissolve in a small amount of water for injection, filter through a filter membrane with a pore size of 0.22 μm, and set aside;

[0036] (3) Under sterile conditions, mix 100g of CSF splenocyte vaccine, 100ml of CSF ST cell vaccine, and 120ml of CSF bovine testicular cell vaccine;

[0037] (4) Mix the material liquid prepared in step (1) (2) (3), add water for injection to 1L, mix evenly, divide the package, and then place it in a freeze-drying box at 4°C at a rate of 2°C / min Cool down to -30°C, keep it for 3h, then turn on the vacuum pump, evacuate the inside of the box to 0.01mbar, then raise the temperature to -4°C within 22h, then raise the temperature to 22°C at a rate of 6°C / h, keep it f...

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Abstract

The invention discloses a swine fever live vaccine compound preparation and a preparation method thereof. The compound preparation is prepared from swine fever spleen lymph vaccine, swine fever ST cell vaccine, swine fever bovine testicle cell vaccine, lactoalbumin hydrolysate, skim milk powder, cane sugar, hydrolysis gelatin, sodium glutamate, mannitol, trehalose and water for injection by stepsof proportional mixing and lyophilization. The compound preparation integrates advantages of the swine fever spleen lymph vaccine and the swine fever cell vaccine to completely induce cellular immunity and humoral immunity and break through maternal antibody hindrance and can be stored at 2-8 DEG C for 36 months. In addition, the compound preparation is effective in swine protection rate and suitable for large-scale production and application.

Description

technical field [0001] The invention belongs to the field of virus vaccine preparation for animals, and in particular relates to a compound preparation of swine fever live vaccine and a preparation method thereof. Background technique [0002] Swine fever is an infectious disease of pigs caused by swine fever virus, which is characterized by high contact and high lethality. Pigs of different ages, sexes and breeds can all be infected with the disease, and it is listed as a first-class animal infectious disease in my country. CSFV is a member of the Pestivirus genus of the Flaviviridae family and is an enveloped positive-sense RNA virus. [0003] The clinical symptoms of swine fever are mainly manifested in the following four types: [0004] (1) Acute swine fever. Mental depression, diarrhea, watery or mushy stool with bloodshot and foul odor, body temperature rising to 40-42°C, oral mucosa, lips and teeth accompanied by ulcer bleeding. Often secondary infection, often ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/12A61K47/42A61K47/46A61K47/26A61K47/18A61K47/10A61P31/14
CPCA61K39/12A61K47/10A61K47/183A61K47/26A61K47/42A61K47/46A61K2039/515A61K2039/57A61K2039/575A61K2039/70A61P31/14C12N2770/36034A61K2300/00
Inventor 池宗棋刘幼英刘小龙陈建瞿晓斌范江钢
Owner 派生特(福州)生物科技有限公司
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