A slice smoking device

Abstract

The invention discloses a slice fumigating device which comprises a piston, a solution cylinder, a nipple sleeve, a silicon rubber sleeve and a clamp groove. The slice fumigating device is safe, simple, convenient and effective, slices can be fumigated in fishing or individually fumigated, experimental efficiency is improved, and harm to human bodies caused by toxic solution is reduced.

Description

technical field [0001] The invention relates to a technique for fumigation of samples, in particular to a slice fumigation device. The slice fumigation device is used for transmission electron microscope observation in medical biology research. Background technique [0002] Transmission electron microscopy plays an important role in medical biology research and is used to observe the ultrastructure of samples. Traditional preparation methods for TEM specimens mostly use chemical or physical methods to fix, dehydrate and dry, embedding and polymerization with embedding agents such as epoxy resin, and use glass knife or diamond knife to ultrathin the embedding block on an ultramicrotome. Slicing, the ultra-thin slices were scooped up onto a grid such as a copper grid, and then the ultra-thin slices were double-stained with uranyl acetate and lead citrate. The specific steps are: use glutaraldehyde fixative to soak the sample for pre-fixation; phosphate buffer to rinse the sa...

AI Technical Summary

Problems solved by technology

But use solutions such as trichloromethane or xylene to carry out the following problems in smoked slices: 1, trichloromethane (Trichloromethane) has another name called chloroform or carbon trichloride, is colorless transparent liquid under normal temperature, has special smell, and quality is heavy, easily Volatile, sensitive to light, reacts with oxygen in the air when exposed to light, and gradually decomposes to generate highly toxic phosgene (carbonyl chloride) and hydrogen chloride, which can enter the human body through the human respiratory system, digestive tract and skin, and has an anesthetic effect. It is easy to cause acute poisoning, headache, dizziness, nausea, vomiting, excitement, hot and humid skin and mucous membrane irritation symptoms in the initial stage, mental disorder, shallow breathing, loss of reflex, coma, etc. in severe cases, even respiratory paralysis, ventricular fibrillation and death Death occurred; 2. Xylene (o-Xylene), also known as 1,2-xylene or o-xylene, is a colorless transparent liquid at room temperature, has a special smell, is flammable, and is volatile. It can be inhaled by the human body or contacted through the skin. Contact with mucous membranes can easily cause acute poisoning, is teratogenic, can cause developmental deformities of the musculoskeletal system, and may also be carcinogenic; 3. Currently, the commonly used method of smoking slices is to use tweezers to hold the filter paper, and use the filter paper to absorb chloroform or xylene. After waiting for the volatile solution, quickly place it near the slices floating on the liquid surface for fumigation. When the filter paper picked up by tweezers absorbs too little solution and places it on top of the slices, the solution may have completely evaporated and the slices cannot be smoked. It needs to be absorbed repeatedly for fumigation, which is time-consuming and labor-intensive, and a large amount of volatile gas will cause harm to the user. However, if the filter paper solution picked up by tweezers is absorbed too much, a large amount of volatile gas will also cause harm to the user. Harmful; 4, currently commonly used tweezers to clamp filter paper to absorb volatile solutions such as chloroform or xylene solution to smoke the slices, the tension of the smoke spread is easy to make the slices drift and difficult to collect, which greatly increases the difficulty of picking the slices; 5 , At present, the commonly used method of tweezers to pick up filter paper to absorb volatile solutions such as chloroform and other solutions for smoking slices often causes the phenomenon of solution corrosion of tweezers

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