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A bone-targeted drug delivery system based on near-infrared region II quantum dots and its preparation method and application

A drug delivery system and quantum dot technology, applied in the field of biomedicine, can solve the problems of cumbersome preparation method, high cost, inability to achieve bone targeting, etc., and achieve the effects of good tracing effect, small side effects, and beneficial to circulation in the body

Active Publication Date: 2021-04-02
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The tumor targeting diagnosis and treatment system of the present invention can realize tumor in situ, real-time, visual, quantitative diagnosis, treatment and evaluation, but in order to improve the specificity of targeting tumors, tumor blood vessel targeting molecules need to be coupled, and the preparation method is cumbersome , high cost, and the tumor targeting diagnosis and treatment system of the present invention cannot achieve bone targeting

Method used

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  • A bone-targeted drug delivery system based on near-infrared region II quantum dots and its preparation method and application
  • A bone-targeted drug delivery system based on near-infrared region II quantum dots and its preparation method and application
  • A bone-targeted drug delivery system based on near-infrared region II quantum dots and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] 0.1mmol of silver diethyldithiocarbamate and 10g of dodecanethiol were mixed in a flask and placed in a N 2 In the atmosphere, the temperature was raised to 200°C and kept for 1h. Finally, after the solution was naturally cooled to room temperature, 50mL of absolute ethanol was added, centrifuged, washed, dispersed in cyclohexane, 0.15g of lipoic acid was added to the above cyclohexane, and then added The same volume of absolute ethanol was ultrasonicated in an ultrasonic cleaner for 4 hours, then centrifuged, and washed with deionized water to obtain water-soluble Ag particles with a particle size of about 5 nm. 2 S quantum dots; 0.25mg of the above Ag 2 S quantum dots were dispersed in 100 μL dimethyl sulfoxide (DMSO), and then 50 μL DMSO solution containing 0.01 mmol NHS was mixed with the above solution; then 50 μL DMSO solution containing 0.01 mmol EDC was added to the above mixed solution, wrapped with aluminum foil , stirred for 1 h, centrifuged, and then disper...

Embodiment 2

[0055] Example 2 In vitro bone targeting experiment

[0056] Ag with a particle size of 10 nm modified with aminopolyethylene glycol on the surface 2 Mouse femurs were labeled with near-infrared II region quantum dots for 3 hours, then washed with PBS to remove free quantum dots, and imaged under a near-infrared region II fluorescent small animal imager.

Embodiment 3

[0060] Example 3 In Vivo Bone Targeting Experiment

[0061] (1) Preparation of adriamycin bone-targeted drug delivery system

[0062] Dissolve 10 mg of doxorubicin hydrochloride in 1 mL of DMSO, add 6 μL of triethylamine, stir for 2 hours, add 5 mg of Ag with a particle size of 10 nm modified with amino PEG on the surface 2 For quantum dots in the near-infrared II region, add 9 mL of PBS buffer solution, stir at room temperature for 15 hours in the dark, transfer the reaction solution into a 100K ultrafiltration centrifuge tube, and centrifuge at 4000 rpm for 30 minutes to remove free doxorubicin, wash with PBS for 3 times, Obtain the adriamycin bone-targeted drug delivery system (NH 2 -PEG-Ag 2 S-DOX).

[0063] (2) Visualized drug delivery

[0064] Mice were anesthetized by intraperitoneal injection of chloral hydrate solution, NH 2 -PEG-Ag 2 S-DOX by 200 μg Ag 2 The input amount of S / mouse was infused into nude mice through the tail vein, and observed in a near-infrar...

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Abstract

The invention provides a bone-targeted drug delivery system based on quantum dots in the near-infrared II region and its preparation method and application. The drug delivery system includes quantum dots in the near-infrared region II. There are amino polyethylene glycols. The particle size of the quantum dots in the near-infrared II region of the present invention is less than 20nm, and can reach bone tissue through the blood marrow barrier. The surface of the quantum dots in the near-infrared II region is modified with aminopolyethylene glycol, which is positively charged, and can interact with bones through electrostatic force The negatively charged hydroxyapatite in the medium is efficiently combined, and the quantum dots only rely on physical effects, and are adsorbed on the bone tissue through dynamic and electrostatic forces, realizing the bone targeting effect, which will not interfere with the therapeutic effect of the drug, and have little side effects. The carrier has fluorescent quantum dots in the near-infrared II region, and the present invention can trace the bone-targeting process through the fluorescent signals in the near-infrared II region, which is beneficial to the pharmacological research of drugs.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a bone-targeted drug delivery system based on near-infrared II region quantum dots and a preparation method and application thereof. Background technique [0002] Bones are the frame of the body, and the health of the bones directly affects the survival and quality of life of the body. In recent years, the incidence of bone diseases such as osteoporosis, osteoarthritis and bone tumors has increased year by year, seriously affecting the quality of life of patients. The bone tissue system is huge and widely distributed in the body. During the treatment of bone diseases, if a conventional drug administration method is used, a certain systemic concentration needs to be maintained to allow the drug to reach the tissue and exert its effect, but it may cause systemic disease. Side effects, drug poisoning Treatment options have certain limitations in the treatment of bone diseases. A...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A61K47/02A61K47/18A61K45/00A61P19/08A61P35/00A61P19/10A61P19/02
CPCA61K45/00A61K47/02A61K47/18A61K49/0054A61K49/0067A61P19/02A61P19/08A61P19/10A61P35/00
Inventor 王强斌陶惠泉
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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