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Toona sinensis extract and preparation method and application thereof

A technology of extract and Chinese toon, applied in the medical field, can solve the problems of different drug effects, different composition and content, no comparison of hypoglycemic effect, etc.

Active Publication Date: 2018-08-14
FUYANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But existing way is to extract active ingredient with whole Chinese toon, because the active ingredient and its content of different organ parts of Chinese toon are all not identical, drug effect can be different, on the other hand, the composition and composition of the non-medicinal ingredient of different organ parts The contents are not exactly the same, especially, the ingredients that play a side effect are different, therefore, it is absolutely necessary to select the organ part with the best drug effect as medicine to improve the drug effect and reduce adverse reactions, but the existing reports only explored the effects of Toona sinensis alone. The hypoglycemic effect of the extract, there is no systematic comparison of the hypoglycemic effect of the extracts from different parts of Toona sinensis, and the optimal organ part is selected

Method used

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  • Toona sinensis extract and preparation method and application thereof
  • Toona sinensis extract and preparation method and application thereof
  • Toona sinensis extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1: the preparation of Taihe Toon extract

[0051] Separately weigh 20g of different organ parts (flowers, young leaves, old leaves, seeds) of Toona sinensis Taihe in a beaker, and use 70% ethanol for ultrasonic-assisted extraction (70°C, 40KHz), repeat twice, and the ratio of extraction material to liquid is 1:30, the time is 60min, combine the filtrates, evaporate and concentrate until there is no ethanol smell, centrifuge at 4000r / min for 20min, add 100mL of distilled water to dilute for later use.

[0052]Take 100g AB-8 macroporous resin, soak it in 95% ethanol for 24 hours, and then pack it into the column. Pass the dilution of crude extracts from different parts of Toona sinensis through the AB-8 resin column at a speed of 8mL / min. , wash the column with 70% ethanol, collect the ethanol eluate, concentrate until there is no alcohol smell, freeze in a -80°C refrigerator for 12h, and then freeze-dry in a freeze dryer to obtain Toona sinensis leaf, flower, ...

Embodiment 2

[0053] Example 2: Extracts from different parts of Toon taihe to α-glucosidase inhibitory activity

[0054] α-glucosidase inhibitors can reduce postprandial blood sugar by delaying the digestion and absorption of carbohydrates. It is characterized by inhibiting the degradation of disaccharides into monosaccharides in the last step of carbohydrate digestion. It can inhibit the process of protein glycosylation and lipid glycosylation, and has the functions of preventing and treating obesity, anti-tumor, anti-virus and immunostimulatory activities.

[0055] Determination of α-glucosidase inhibitory activity Reference method with minor modifications [1] . The specific steps are: mix the sample, potassium phosphate buffer (0.1mol L -1 , pH 6.8) each 240μL mixed, add α-glucosidase (0.25U·m L －1 ) was fully shaken until uniform, and after reacting in a water bath at 37°C for 5 minutes, 60 μL of 5mmoL·L was added to the drug group and the positive control group - 1 pNPG, after en...

Embodiment 3

[0067] Embodiment 3: Determination of Taihe Toona sinensis different parts extract to α-amylase inhibitory activity

[0068] Determination of Alpha-Amylase Inhibitory Activity References [2] The method was slightly modified, and the specific steps were as follows: respectively use 0.1mol L -1 , pH = 6.8 phosphate buffer solution dubbed the required concentration.

[0069] Take 100 μL α-amylase solution (10 U m L -1 , pH=6.8) into a 10mL test tube, add 300μL of the test solution (concentrations are 0.5mg / mL, 1mg / mL, 1.5mg / mL, 2 mg / mL, 3.5mg / mL, 3mg / mL), shake for 30s Mix well and activate in a 37°C water bath for 10 minutes. Then add 200 μL of starch solution (1%), shake quickly until uniform, then place in a 37°C water bath for 10 minutes, finally add 200 μL of DNS chromogenic reagent, react in a boiling water bath for 5 minutes, take out the cold water bath to room temperature, and finally add 1 mL of distilled water , and each treatment was repeated 3 times.

[0070] Me...

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Abstract

The invention discloses a preparation method of a toona sinensis extract. The preparation method comprises the steps that 1, a toona sinensis material is taken to be subjected to ultrasonic-assisted extraction in ethyl alcohol; 2, filter liquid is taken to evaporate ethyl alcohol, so that filter residue is obtained; 3, the filter residue is centrifuged, so that a toona sinensis crude extract is obtained, and distilled water is added to dilute the toona sinensis crude extract into toona sinensis extract diluted liquid for stand-by application; and 4, the toona sinensis extract diluted liquid ispurified. The toona sinensis extract prepared by adopting the preparation method, particularly a toona sinensis flower extract, can effectively reduce the activity of alpha-diastase and alpha-glucosidase in the body, reduces the blood sugar content in the body, and accordingly is the optical selection for preparing drugs or health care products for treating or relieving diabetes.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to Chinese toon extract, its preparation method and use, in particular to the Chinese toon flower extract, its preparation method and its use in treating or alleviating diabetes. Background technique [0002] Chinese toon Toonasinensis (A.Juss.) Roem. is a plant of the family Meliaceae, and its leaves are rich in biological active ingredients such as flavonoids, saponins, and phenols. The history of planting Chinese toon in Taihe County, Anhui Province is very long, and its quality is high. It was used as a tribute in the Tang Dynasty, and it was imperially named "Gongchun" in the Qing Dynasty. Chinese toon flower, also known as toon tree and toon flower, has the effect of expelling wind, dispelling cold and relieving pain, and is often used to treat headaches, migraines, rheumatic joint pain and other diseases. There is a folk saying that Chinese toon decoction can prevent diabetes. The anti...

Claims

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Application Information

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IPC IPC(8): A61K36/58A61P3/10A23L33/105
CPCA23V2002/00A61K36/58A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55A23L33/105A61P3/10A23V2200/328A23V2250/21
Inventor 杨京霞屈长青陶恒港王啸洋李玉
Owner FUYANG NORMAL UNIVERSITY
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