Microbial liquid foaming agent and preparation method thereof
A technology of microbial bacteria liquid and foaming agent, which is applied in chemical instruments and methods, chemical/physical processes, dissolution, etc., can solve the problems of pollution, energy consumption, poor performance, etc., and achieve high cost, low cost, foaming large amount of effect
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Embodiment 1
[0039] The masses of the components are respectively 10 g of glucose, 10 g of peptone, 5 g of yeast extract powder, 10 g of yeast seed liquid, and 0.2 g of foam stabilizer. The foam stabilizer is gelatin.
[0040] The preparation method is:
[0041] (1) Preparation of original solution: add 100ml of water to the Erlenmeyer flask, then add 10g glucose, 10g peptone and 5g yeast extract powder to the Erlenmeyer flask, seal the Erlenmeyer flask with air-permeable gauze, and then seal the Erlenmeyer flask High-pressure high-temperature steam sterilization for 25 minutes, the air pressure of high-pressure high-temperature sterilization is 0.104MPa, and the temperature is 121°C.
[0042] (2) Bacterial liquid inoculation: After the sterilization, take out the Erlenmeyer flask and place it on the aseptic operating table and turn on the ultraviolet light to sterilize for 30 minutes, then cool to 20-30°C; Fraction 10% is used to inoculate the liquid in the Erlenmeyer flask as a bacteri...
Embodiment 2
[0047] The quality of the components is 10g of glucose, 15g of peptone, 10g of yeast extract powder, 5g of yeast seed solution and 0.4g of foam stabilizer. The foam stabilizer is gelatin.
[0048] The preparation method is:
[0049] (1) Preparation of the original solution: add 100ml of water to the Erlenmeyer flask, then add 10g of glucose, 15g of peptone and 10g of yeast extract powder to the Erlenmeyer flask, seal the Erlenmeyer flask with air-permeable gauze, and then seal the Erlenmeyer flask High-pressure high-temperature steam sterilization for 25 minutes, the air pressure of high-pressure high-temperature sterilization is 0.104MPa, and the temperature is 121°C.
[0050] (2) Bacterial liquid inoculation: After the sterilization, take out the Erlenmeyer flask and place it on the aseptic operating table and turn on the ultraviolet light to sterilize for 30 minutes, then cool to 20-30°C; Fraction 10% is used to inoculate the liquid in the Erlenmeyer flask as a bacterial ...
Embodiment 3
[0055]The quality of the components is 15g of glucose, 15g of peptone, 5g of yeast extract powder, 15g of yeast seed liquid and 1g of foam stabilizer. The foam stabilizer is gelatin.
[0056] (1) Preparation of original solution: add 100ml of water to the Erlenmeyer flask, then add 15g of glucose, 15g of peptone and 5g of yeast extract powder to the Erlenmeyer flask, seal the Erlenmeyer flask with air-permeable gauze, and then seal the Erlenmeyer flask High-pressure high-temperature steam sterilization for 25 minutes, the air pressure of high-pressure high-temperature sterilization is 0.104MPa, and the temperature is 121°C.
[0057] (2) Bacterial liquid inoculation: After the sterilization, take out the Erlenmeyer flask and place it on the aseptic operating table and turn on the ultraviolet light to sterilize for 30 minutes, then cool to 20-30°C; Fraction 10% is used to inoculate the liquid in the Erlenmeyer flask as a bacterial solution, and the bacterial solution is adjuste...
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