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Yeast extract liquid, preparation method, and application of same

A technology of yeast dipping and yeast dipping powder, applied in the field of biological products, can solve the problems of unstable quality, difficult concentration of production, contamination of miscellaneous bacteria, etc., to improve product yield and product quality, shorten the heating time of materials, reduce heat damage effect

Active Publication Date: 2018-07-27
ANGELYEAST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, users of large logarithmic mycoplasma culture and animal cell culture adopt active dry yeast to purchase by themselves, and then process and prepare yeast infusion by themselves, and add it to the medium, which is not conducive to promoting research and development or production efficiency, and the nutritional components between batches cannot be controlled. In addition, if the processing time in the preparation is too long (more than 24-36 hours), the probability of contamination with bacteria will increase, and the color of the yeast extract product contaminated with bacteria in the preparation process will be dark and have sediment
Affected by such factors, the yeast extracts prepared vary greatly among batches, and the quality is extremely unstable, which affects the normal progress of various cultivation work
[0004] The Chinese patent CN101810319A disclosed in the related field discloses the preparation process of related yeast dipping juice, but the raw material selected in this process is brewer's yeast, using brewer's yeast or beer waste yeast mud as the production raw material, more washing, debittering , the cumbersome process of deodorization
In addition, the production of yeast extract powder according to the above-mentioned disclosed process also has the following defects: if large-scale production of beer yeast extract powder raw materials requires a large amount of beer yeast sludge, it is difficult to purchase, keep fresh and transport, and production is not easy to concentrate
2) Due to the fact that the production raw materials cannot be controlled from the source, the balance of brewer's yeast extract powder will inevitably lead to the quality stability of each batch of products

Method used

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  • Yeast extract liquid, preparation method, and application of same
  • Yeast extract liquid, preparation method, and application of same
  • Yeast extract liquid, preparation method, and application of same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] (1) Preparation method of yeast infusion

[0073] Embodiment 1 provides a method for preparing yeast infusion using vacuum and low temperature. Wherein, the yeast infusion is cultured at a high density using Saccharomyces cerevisiae until the wet weight of yeast cells is 220 g / L.

[0074] Among them, the method of cultivating yeast cells at high density is as follows:

[0075] a. Treatment of fermented raw materials: remove slag and precipitate molasses, add concentrated sulfuric acid to acidify to pH 2.0, and hydrolyze molasses to obtain fermented molasses with reducing sugar weight accounting for 20%; yeast extract powder is added with water to make a solution with a concentration of 20%; The molasses and yeast infusion solution was sterilized at 110°C for 10 minutes.

[0076] b. Seed culture: first-level seed culture, insert a ring of bacteria (Saccharomyces cerevisiae FX-2) into 200mL first-level medium from the slant of S. For seed culture, insert the seeds of t...

Embodiment 2

[0120] Embodiment 2 provides a method for preparing yeast infusion using vacuum and low temperature. Wherein, the yeast infusion is cultured at a high density using Saccharomyces cerevisiae until the wet weight of yeast cells is 280 g / L.

[0121] Among them, the method of cultivating yeast cells at high density is as follows:

[0122] a. Fermentation raw material treatment: molasses removes slag and precipitates, adds concentrated hydrochloric acid to acidify to pH2.5, hydrolyzes molasses to obtain fermented molasses with reducing sugar weight accounting for 25%, yeast extract powder is added with water to form a solution with a concentration of 25%; The molasses and yeast infusion solution was sterilized at 110°C for 10 minutes.

[0123] b. Seed culture: first-level seed culture, insert a ring of bacteria (Saccharomyces cerevisiae FX-2) into 200mL first-level medium from the slant of S. For seed culture, insert 1L of the secondary medium according to the inoculation amount ...

Embodiment 3

[0136] Embodiment 3 provides a method for preparing yeast infusion using vacuum and low temperature. Wherein, the yeast extract is cultured at a high density using Saccharomyces cerevisiae until the wet weight of yeast cells is 240 g / L.

[0137] Among them, the method of cultivating yeast cells at high density is as follows:

[0138] a. Fermentation raw material treatment: molasses deslagging and precipitation, adding concentrated nitric acid to acidify to pH 3.5, so that molasses is hydrolyzed to obtain fermented molasses with reducing sugar weight accounting for 22%, yeast extract powder is added with water to form a solution with a concentration of 22%; The molasses and yeast infusion solution was sterilized at 110°C for 10 minutes.

[0139] b. Seed culture: first-level seed culture, insert a ring of bacteria (Saccharomyces cerevisiae FX-2) from the strain storage slope into 250mL first-level medium, and cultivate at 32°C and 300rpm for 26h; second-level seed culture , ac...

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Abstract

The invention relates to the technical field of bio-products, and especially relates to a yeast extract liquid, a preparation method, and an application of same. The invention provides the method of high-effectively preparing the yeast extract liquid in vacuum, which includes steps of: 1) preparing yeast milk by culturing saccharomyces cerevisiae; 2) autolysis: regulating the yeast milk to weak-acidity to perform autolysis; 3) vacuum extraction: performing vacuum treatment to the autolyzed material and heating the material until boiling; 4) separating the liquid to obtain the yeast extract liquid. The invention also discloses the yeast extract liquid prepared through the method. The method is low in reaction temperature, is high in vacuum degree, is short in material heating time and is high in extraction degree, is very suitable for extracting substances having high boiling point and thermosensitivity and being easy to oxidize. The method also can reduce thermal damage on thermosensitive substance effectively and can greatly increase yield and improve quality of the product.

Description

technical field [0001] The invention relates to the technical field of biological products, in particular to a yeast extract and its preparation method and application, in particular to a method and application for efficiently preparing yeast extract using vacuum and low temperature. Background technique [0002] Yeast milk is a pure natural, pollution-free, biological health source of nutrition, with more than 50 kinds of nutrients, including complete protein, vitamin B group, nucleic acid, etc. necessary for the human body, and is praised as the most attractive nutrition by the nutritional circles Taste. The water content in yeast cells is 65-70%, of which about 15% is free water; 35-45% of carbohydrates, mainly in the form of polysaccharides; 35-45% of proteins, including monomeric proteins and binding Protein; 3-5% fat, mainly lecithin and sterol; 5-10% ash, mainly potassium, phosphorus content. In addition, it also contains a variety of vitamins, with the most B vitam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/06C12N1/18C12R1/865
CPCC12N1/063C12N1/18
Inventor 李啸俞学锋李知洪谈亚丽
Owner ANGELYEAST CO LTD
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