Process for extracting, preparing and purifying gamma methyllinolenate from algae
A technology of methyl linolenate and technology, which is applied in the field of γ-linolenic acid methyl ester, can solve the problems of high cost industrial production, high cost, and high cost, and achieve the effects of avoiding GLA oxidation, short heating time, and high vacuum degree
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0017] Add 2000g of raw material Spirulina maxia (Spirulina maxia) algae powder (containing about 14.5g of GLA), add 1000mL of methanol to homogenize, and use CO at 20MPa and 40°C 2 Carry out supercritical extraction as solvent, obtain 56g total lipid after concentrating, total lipid is dissolved in the methyl alcohol containing 5% chloroacetyl of 12 times its volume, under the protection of nitrogen atmosphere, 80 ℃ reflux reaction after 2 hours, cool to At room temperature, add twice the volume of 0.3% sodium carbonate solution, wash, centrifuge to take the upper oil layer, concentrate to obtain the total fatty acid methyl esters and recover methanol, and the total methyl esters are molecularly distilled at 160°C under a high vacuum of 6-10Pa, and the distillate is collected Add three times the volume of acetone to the distillate, and then equilibrate crystallization at 4°C, -10°C, and -30°C for 24 hours to remove part of the saturated fatty acid methyl ester. After each crys...
Embodiment 2
[0019] Add 2000 grams of raw material Spirulina maxia (Spirulina maxia) algae powder (containing about 14.5 g of GLA), add 4000 ml of methanol to homogenate, and use CO2 as a solvent for supercritical extraction at 50 MPa and 60 ° C. After concentration, you can get 98g total lipids, the total lipids are dissolved in 12 times its volume of methanol containing 5% chloroacetyl, under the protection of nitrogen atmosphere, 80 ° C reflux reaction for 2 hours, cooled to room temperature, add twice the volume of 0.3% sodium carbonate Solution, centrifuge to take the upper oil layer, concentrate to obtain the total fatty acid methyl ester and recover methanol, the total methyl ester is molecularly distilled at 160°C under a high vacuum of 6-10Pa, collect the distillate, and add three times the volume of acetone to the distillate , followed by equilibrium crystallization at 4°C, -10°C, and -30°C for 24 hours to remove part of saturated fatty acid methyl esters, and filtered to remove c...
Embodiment 3
[0022] 2,000 grams of raw material freeze-dried Nostoc sp algae powder (containing about 36.4 g of GLA), was added to 4,000 ml of methanol for homogenization, and then supercritical extraction was carried out at 40 MPa and 60 ° C using CO2 as a solvent to obtain 103 g of total lipids class, the following steps are as in Example 2, and finally obtained 4.8 g of GLA methyl ester with a purity of more than 90%.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com