Graphene oxide targeted drug carrier material as well as preparation method and application thereof

A carrier material, graphene technology, used in drug combinations, pharmaceutical formulations, anti-tumor drugs, etc., can solve the problems of large human body, toxic side effects, low drug loading rate and drug release, and achieve high drug loading rate, toxic and side effects. The effect of small side effects, high drug loading rate and high drug release

Inactive Publication Date: 2018-06-08
GUANGXI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Based on this, it is necessary to provide a graphene oxide-targeted drug carrier material and its preparation method and application in view of the technical problems that the above-mentioned drug carrier has relatively large toxic and side effects on the human body, and the drug loading rate and drug release amount are low.

Method used

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  • Graphene oxide targeted drug carrier material as well as preparation method and application thereof
  • Graphene oxide targeted drug carrier material as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] This embodiment includes the following steps to prepare graphene oxide targeting drug carrier.

[0038] 1) Take 0.5g RGDF polypeptide molecule and add it to a phosphate buffer solution with a pH of 5.2, and then add EDC and NHS for activation, wherein, the mass ratio of RGDF polypeptide molecule: EDC:NHS is 1:10:2, and the activation time is After 20 minutes, the activated RGDF polypeptide solution was obtained;

[0039] 2) Take 5g of aminated graphene oxide material and add it to the above-mentioned activated RGDF polypeptide solution, and perform ultrasonic treatment to obtain a mixture;

[0040] 3) reacting the mixture obtained above at room temperature for 2 hours, and performing centrifugal purification on the reaction product at a centrifugal rate of 10,000 r / min to obtain a graphene oxide targeting drug carrier material;

[0041] 4) Add 2g of graphene oxide targeting drug carrier material to 50mL of deionized water for ultrasonic dissolution, mix with 80mg of do...

Embodiment 2

[0043] This embodiment includes the following steps to prepare graphene oxide targeting drug carrier.

[0044] 1) Take 1.0 g of RGDF polypeptide molecules and add them to the MES washing solution with a pH of 6.4, and then add EDC and NHS for activation, wherein the mass ratio of RGDF polypeptide molecules: EDC:NHS is 1:8:4, and the activation time is After 25 minutes, the activated RGDF polypeptide solution was obtained;

[0045] 2) Take 8g of aminated graphene oxide material and add it to the above-mentioned activated RGDF polypeptide solution, and perform ultrasonic treatment to obtain a mixture;

[0046] 3) reacting the mixture obtained above for 3 hours at normal temperature, and performing centrifugal purification on the reaction product at a centrifugal rate of 20000r / min to obtain a graphene oxide targeting drug carrier material;

[0047] 4) Add 4g of graphene oxide targeting drug carrier material to 200mL deionized water for ultrasonic dissolution, mix with 80mg cape...

Embodiment 3

[0049] 1) Take 1.5g RGDF polypeptide molecule and add it to PBS buffer solution with pH 7.2, then add EDC and NHS for activation, wherein, the mass ratio of RGDF polypeptide molecule: EDC:NHS is 1:6:1, and the activation time is After 30 minutes, the activated RGDF polypeptide solution was obtained;

[0050]2) Add 10 g of aminated graphene oxide material to the above-mentioned activated RGDF polypeptide solution, and perform ultrasonic treatment to obtain a mixture;

[0051] 3) reacting the mixture obtained above for 3 hours at normal temperature, and performing centrifugal purification on the reaction product at a centrifugal rate of 15000r / min to obtain a graphene oxide targeting drug carrier material;

[0052] 4) Take 6g of graphene oxide targeting drug carrier material and add it to 400mL of deionized water for ultrasonic dissolution, mix with 80mg of Iressa dissolved in 200mL of ethanol, after ultrasonic treatment, stir at room temperature for 24h, use dialysis to remove ...

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Abstract

The invention relates to a graphene oxide targeted drug carrier material. The graphene oxide targeted drug carrier material contains RGDF polypeptide molecules, a graphene oxide material and an anti-tumor drug loaded by graphene oxide, wherein the RGDF polypeptide molecules and the graphene oxide material are linked through covalent bonds; active functional groups of graphene oxide include amino and / or hydroxy, carboxyl and epoxy group; and the graphene oxide material is a product prepared by carrying out amidation reaction on the RGDF polypeptide molecules and aminated graphene oxide. A drugcarrier provided by the invention has biocompatibility, small toxic and side effects, good dissolving property and high drug loading ratio; after entering a human body, the carrier loading the anti-tumor drug can stably circulate in the human body, and the anti-tumor drug can be enriched at tumor cells by virtue of the polypeptide molecules, so that the targeting property is realized; and besides,receptor binding sites on polypeptide are not exposed outside, so that the stability of the anti-tumor polypeptide molecules in the human body is improved.

Description

technical field [0001] The invention relates to the field of nano-targeted drug carriers, in particular to a graphene oxide targeted drug carrier material and its preparation method and application. Background technique [0002] With the progress of industrial civilization, the morbidity and mortality of malignant tumors are increasing year by year in both developed and developing countries. The World Health Organization (WHO) published a set of statistical data at the beginning of this century. In 2000, the annual incidence of malignant tumors in the world exceeded 10 million, and the death toll reached 7 million. It is estimated that by 2020, the annual incidence of malignant tumors in the world will reach 15 million. , the annual death toll will exceed 10 million. At present, the number of deaths due to malignant tumors in my country's urban population has exceeded the number of deaths due to cardiovascular and cerebrovascular diseases, and has become the largest cause o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/18A61K47/04A61P35/00
CPCA61K47/183A61K47/02
Inventor 赵立春
Owner GUANGXI UNIV OF CHINESE MEDICINE
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