Pseudomonas aeruginosa bacteriophage with wide lysis spectrum and disinfection application of pseudomonas aeruginosa bacteriophage

A Pseudomonas aeruginosa and bacteriophage technology, applied in the field of phages, can solve the problems that mink farms seldom spray for disinfection, cannot effectively kill Pseudomonas aeruginosa, and corrosiveness, and achieve excellent disinfection effect, no irritation and corrosion, Mortality reduction effect

Inactive Publication Date: 2018-05-25
QINGDAO PHAGEPHARM BIO TECH CO LTD
View PDF5 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mink farms rarely carry out spray disinfection, and conventional disinfectants cannot effectively kill Pseudomonas aeruginosa, and have side effects such as irritation and corrosion
Domestic researchers have done disinfection tests on mink in a closed environment, but there is no report on the environment disinfection of mink farms with phages to control the occurrence of mink hemorrhagic pneumonia

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pseudomonas aeruginosa bacteriophage with wide lysis spectrum and disinfection application of pseudomonas aeruginosa bacteriophage
  • Pseudomonas aeruginosa bacteriophage with wide lysis spectrum and disinfection application of pseudomonas aeruginosa bacteriophage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Isolation and Identification of Phage

[0033] (1) Recovery and cultivation of strains

[0034] Pick out the bacterial solution of Pseudomonas aeruginosa, line it in three zones on the common agar medium, isolate a single colony, and culture it in a 37°C incubator for 16-24h.

[0035] (2) Preparation of bacterial proliferation solution

[0036] A single colony was picked, inoculated into a test tube containing 5 mL of LB broth, and cultured in an air shaker at 37° C. with shaking at 200 rpm for 12 hours to obtain a single bacterial suspension of Pseudomonas aeruginosa.

[0037] (3) Preparation of mixed bacterial suspension

[0038] Take 200 μL of a single bacterial solution, add it to a Erlenmeyer flask filled with 100 mL of LB broth, and incubate in an air shaker at 37°C at 200 rpm for 12 hours.

[0039] (4) Preliminary treatment of feces, litter, fur, etc.

[0040] Take feces samples, litter and fur of mink in the Erlenmeyer flasks filled with LB broth, ...

Embodiment 2

[0048] The mensuration of embodiment 2 phage lysis spectrum

[0049] The single-spot method was used to measure the lysis profile of the phage, and the steps were as follows: pick up a ring of Pseudomonas aeruginosa liquid with an inoculation loop, separate a single colony by streaking on an ordinary agar plate, and incubate in a 37°C incubator for 16-18h. Use sterile tweezers to pick up the sterilized white tip to pick up a single colony, inoculate in a test tube containing 5mL of nutrient broth, culture at 37°C and 200rpm in an air bath for 12h, and obtain a single bacterial suspension. Take 200 μL of bacterial suspension and evenly spread it on an ordinary agar plate, take 1 μL of phage proliferation solution and drop it on different positions of the plate, and after natural drying, place it in a 37°C incubator for 6-8 hours and observe the results.

[0050] The results showed that 17 strains of Pseudomonas aeruginosa were used as host bacteria to measure the lysis spectrum...

Embodiment 3

[0051] Embodiment 3 Transmission electron microscope observes the morphology of phage

[0052] (1) Sample preparation

[0053] Take 200 μL of host bacteria solution and phage proliferation solution respectively, add them to a test tube containing 5 mL of nutrient broth, and incubate with shaking at 37°C for 2-3 hours, until the mixed solution changes from turbid to clear and there are debris, add 200 μL chloroform, continue to shake and cultivate for 30 minutes, and centrifuge at 10,000 rpm for 5 minutes to obtain a phage proliferation solution, and filter the prepared phage proliferation solution with a 0.22 μm filter to remove possible residual bacteria.

[0054] The results showed that the cultures added with phages could be clarified within 3 hours, which indicated that the phages had a strong ability to lyse the host bacteria.

[0055] (2) Processing of samples

[0056] Take 20 μL of the sample suspension and drop it on the microporous copper sheet, settle for about 15 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the field of bioengineering, and provides a bacteriophage with environmental disinfection capacity and application of the bacteriophage. A bacteriophage monomer is a pseudomonas aeruginosa bacteriophage, the Latin name of the bacteriophage monomer is P. aeruginosaphage, the bacteriophage monomer is named as Lp14 and has broad-spectrum bactericidal ability for pseudomonas aeruginosa, the bacteriophage Lp14 is preserved in the General Microbiology Center of the China Committee for Culture Collection of Microorganisms on March 30th, 2017, and the preservation number of the bacteriophage Lp14 is CGMCC No.13791. The bacteriophage and the application have the advantages that strong pseudomonas aeruginosa lysis effects can be realized by the bacteriophage, and bacteriophage sources can be provided to bacteriophage industrial production and sterilization and disinfection; the bacteriophage further can be used as a safe biological disinfectant for disinfecting mink farmenvironments, and accordingly hemorrhagic pneumonia due to pseudomonas aeruginosa during mink breeding can be reduced; the bacteriophage can be used for industrial production, can be specifically amplified from the pseudomonas aeruginosa which is a host bacterium, and can be used as the biological disinfectant for disinfecting the mink farm environments.

Description

technical field [0001] The invention belongs to the field of bioengineering, and in particular relates to a bacteriophage strain capable of disinfecting the environment and its application. Background technique [0002] Pseudomonas aeruginosa (PA), also known as Pseudomonas aeruginosa, is one of the main pathogens causing hemorrhagic pneumonia in minks. It is widely distributed in nature and can cause acute infections in minks, foxes, and chickens. [0003] In recent years, with the rapid development of my country's mink breeding industry and the continuous expansion of the breeding scale, mink hemorrhagic pneumonia caused by Pseudomonas aeruginosa often presents endemic and seasonal outbreaks, and the case fatality rate is even as high as 50%. caused huge economic losses. With the increasing antibiotic resistance, infections caused by Pseudomonas aeruginosa cannot be effectively treated with conventional antibiotics, especially the sensitivity to aminoglycosides, fluoroquin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N7/00A61K35/76A61P31/04A01N63/00A01P1/00A01N63/40
CPCA01N63/00A61K35/76C12N7/00C12N2795/00021C12N2795/00031A61P31/04A01N63/40
Inventor 任慧英孙虎芝于晓研潘强刘慧
Owner QINGDAO PHAGEPHARM BIO TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap