Method for identification and systematic classification of citrus variety based on 21 characteristic component content

Abstract

The invention belongs to the filed of analytical chemistry, particularly relates to a method for the identification and systematic classification of citrus variety based on 21 characteristic componentcontent. The method comprises the following steps: selecting citrus standard samples, measuring the contents of 17 characteristic components in the standard samples and the percent ratio of the content of 4 organic acids to the content of the total acid, utilizing the content and percent ratio as indexes to build a sample matrix, applying a principal component analysis method and a clustering methodology method, selecting the chemical component with largest weight in principal component as a distinguishing factor, utilizing the distinguishing factor as an independent variable, calculating thesquared euclidean distance among the citrus standard samples with the same variety, obtaining the squared euclidean distance range of the standard samples, measuring the characteristic component content of the distinguishing factor of samples to be measured, respectively calculating the squared euclidean distance range of the characteristic component content of the distinguishing factor of samples to be measured and the citrus standard samples and judging the variety of samples to be measured. The method can realize the effective distinguishing among different citrus variety, especially amongpomelo, citrus reticulate and orange and the like, and is an objective and reliable method for citrus classification.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, specifically a method for identifying and systematically classifying citrus varieties based on the contents of 21 characteristic components. Background technique [0002] Citrus is the largest fruit in the world and one of the main fruits grown in China. In 2015, my country's citrus cultivation area ranked first in the world, and its output ranked third. In 2015, the national citrus area was 2,513,000 hectares, and the output was 36,600,800 tons, which occupies a very important position in the rural economy in southern my country. Because citrus is a perennial woody plant, it is easy to hybridize between species and genus, has a high budding frequency and a long history of cultivation, making citrus germplasm resources very rich and complex, and many closely related varieties have little difference in agronomic traits, or even only a single species. Insignificant variation in traits. Variety...

AI Technical Summary

Problems solved by technology

However, at present, the analysis of the results of multiple experiments by most researchers is only on the intuitive description of the results of molecular markers such as AFLP and ISSR, without in-depth quantitative analysis. more accurate judgment

For each variety, there is no rapid and efficient measure for variety identification using phenolic substance content and organic acid ratio as markers combined with cluster analysis and principal component analysis methods.

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