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Strains that can be used for the degumming of hemp microorganisms and the degumming process of hemp

A hemp and degumming technology, applied in the direction of microorganism-based methods, microorganisms, microorganisms, etc., can solve the problems of long degumming cycle, complicated process, environmental pollution, etc., achieve the effects of rough culture conditions, simple degumming process, and reduce production costs

Active Publication Date: 2019-02-12
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a strain that can be used for the degumming of hemp microorganisms and its degumming process to solve the problems of long degumming cycle, complicated process, environmental pollution and high cost in the existing degumming process

Method used

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  • Strains that can be used for the degumming of hemp microorganisms and the degumming process of hemp

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Effect test

Embodiment 1

[0033] The present invention can be used for degumming strains of hemp microorganisms. The strain is a plant spoilage fungus with a preservation number of CGMCC 14601, named as a plant spoilage fungus PW (Pectobacterium wasabiae IBFC2016), which was deposited in China's Common Microorganism Bacteria on September 08, 2017. species collection management center.

[0034] The screening and identification method of plant spoilage bacteria PW comprises the following steps:

[0035] (1) Sampling and processing: Take 10 g of the sludge sample from the retting pond, place it in a conical flask equipped with an appropriate amount of glass beads, add 200 mL of sterile water, mix at 180 rpm for 20 min, filter and remove the residue with multi-layer gauze to obtain a suspension;

[0036](2) Enrichment screening: take 10mL of suspension, 150mL of tap water and 15g of hemp bast, mix them in a conical flask, ferment at 35°C and shake at 180rpm until the fibers are dispersed; take 10mL of the ...

Embodiment 2

[0040] The plant spoilage bacteria PW obtained in Example 1 was applied, and on December 18-25, 2016, the bacterial suspension preparation and ramie biological degumming technology were respectively implemented in Hunan Lierkang Biological Company and Hanshou Ziyang Textile Raw Materials Co., Ltd. , the technological route such as figure 1 As shown, the specific steps include:

[0041] (1) Preparation of bacterial strain suspension:

[0042] Inoculate a ring of slant-preserved plant spoilage bacteria PW into a broth medium test tube, activate at 35°C for 8 hours, and after dilution and coating, grow on a culture plate for 24 hours to obtain a typical single colony; select a typical single colony and insert it into the In a 5mL test tube of broth medium, culture at 35°C for 6 hours; inoculate 2% of the inoculum into a 200mL Erlenmeyer flask containing sterile broth medium, and culture at 35°C for 6 hours with shaking at 180rpm; Transfer the amount to the seed tank containing ...

Embodiment 3

[0054] The plant spoilage bacteria PW obtained in Example 1 was applied, and on February 16-22, 2017, the bacterial suspension preparation and hemp biological degumming technology were implemented in the Chinese Academy of Agricultural Sciences' hemp processing enzyme preparation pilot workshop. The specific steps include:

[0055] (1) Preparation of bacterial strain suspension:

[0056] Inoculate a ring of slant-preserved plant spoilage bacteria PW into a broth medium test tube, activate at 35°C for 8 hours, and after dilution and coating, grow on a culture plate for 24 hours to obtain a typical single colony; select a typical single colony and insert it into the In a test tube of 5mL broth medium, culture at 35°C for 6h; transfer to a seed tank containing sterile broth medium at 2% inoculum size, 35°C, 180rpm, 0.4MPa compressed air flow rate 0.3m 3 / m 3 h, cultivated for 6 hours, and obtained 10L of bacterial suspension; under the same conditions, obtained 120L of expansion...

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Abstract

The invention discloses a bacterial strain that can be used for the degumming of hemp microorganisms. The bacterial strain is a plant spoilage fungus with the preservation number CGMCC 14601, and is named as the plant spoilage fungus PW. It also provides a process for degumming hemp using plant spoilage bacteria PW, which is followed by the preparation of bacterial suspension, pretreatment of hemp raw materials, cyclic soaking and inoculation, fermentation, inactivation and washing of hemp, and finally pure hemp fiber is obtained . The plant spoilage bacteria PW of the present invention can produce extracellular pectinase, mannanase and xylanase, the enzyme activity is high, the culture conditions are rough, the growth speed is fast, and the degumming cycle is short; the degumming process of the present invention is simple, the condition is mild, The pollutant treatment load is 80% lower than that of chemical degumming, and the processing cost is reduced by about 20%. A new type of circulating hemp biological degumming process is adopted, and the bacteria liquid cultivated once can be recycled for many times, which improves the concentration and use efficiency of the bacteria liquid, shortens the The technological process reduces production costs and reduces the risk of strain variation and inactivation.

Description

technical field [0001] The invention belongs to the technical field of hemp degumming, and in particular relates to a bacterial strain that can be used for degumming hemp microorganisms and a hemp degumming process. Background technique [0002] The bast part of hemp crops such as ramie, kenaf, jute, hemp, etc., contains 20% to 40% of non-cellulosic substances, including lignin (2% to 5%), pectin (4% to 8%) and hemifiber Elements (12% to 18%) and other substances, these substances are embedded between cells and in the cell wall, the structure is very complex. Mannan and xylan are important bridges connecting cellulose and lignin. They are often linked to cellulose by covalent bonds, and linked to lignin by hydrogen bonds or other non-covalent bonds. Lignin is composed of Polyphenylpropane, etc.; pectin is mainly composed of polygalacturonic acid, and the degradation product is galacturonic acid; hemicellulose is a kind of substance with very complex structure and compositio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20D01C1/02C12R1/01
CPCC12N1/205C12R2001/01D01C1/02
Inventor 段盛文彭源德成莉凤冯湘沅郑科
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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