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Preparation of three-dimensional graphene nano composite material and method for chirally recognizing tryptophan (Trp)

A nano-composite material and graphene technology, which is applied in the preparation of three-dimensional graphene nano-composite materials and its chiral recognition of tryptophan, can solve the problems of inconspicuous recognition effect, long recognition time, and low sensitivity, and achieve excellent electronic performance. Chemical properties, high sensitivity, and good selectivity

Active Publication Date: 2018-03-06
SHANXI UNIV
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  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to overcome the shortcomings of the prior art, to provide a preparation of a three-dimensional graphene nanocomposite material and its chiral recognition method for tryptophan, to solve the problems of long recognition time, complexity, high cost, Technical problems such as low sensitivity and inconspicuous recognition effect

Method used

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  • Preparation of three-dimensional graphene nano composite material and method for chirally recognizing tryptophan (Trp)
  • Preparation of three-dimensional graphene nano composite material and method for chirally recognizing tryptophan (Trp)
  • Preparation of three-dimensional graphene nano composite material and method for chirally recognizing tryptophan (Trp)

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Embodiment 1

[0026] A preparation method of a three-dimensional graphene nanocomposite material, comprising the following steps:

[0027] 1) Preparation of three-dimensional graphene (3D-G):

[0028] 40 mg of graphene oxide was ultrasonically treated in 20 mL of deionized water for 1 hour to prepare a graphene oxide dispersion, which was then placed in a container

[0029] Dissolve 80 mg of L-cysteine ​​in the graphene oxide dispersion, then add 500 μL of concentrated ammonia water dropwise, and stir evenly;

[0030] Finally, put the above-mentioned reactants in a microwave oven for 5 minutes to react to obtain a black suspension; after cooling to room temperature, the black suspension was subjected to suction filtration, during the process of suction filtration, it was repeatedly washed with deionized water, and freeze-dried for 24 hours to obtain Three-dimensional graphene powder;

[0031] 2) Preparation of three-dimensional graphene nanocomposite (3D-G / HP-β-CD):

[0032] First, 20 mg...

Embodiment 2

[0038] A method for chiral recognition of tryptophan by a three-dimensional graphene nanocomposite material, comprising the steps of:

[0039] 1) Take the 3D-G / HP-β-CD powder prepared in Example 1 in the mixture of ethanol and water, and ultrasonically treat it to make a 1 mg / mL dispersion; then take 5 μL of 3D-G / HP-β -CD dispersion liquid was drop-coated on the surface of the polished glassy carbon electrode and dried to obtain a 3D-G / HP-β-CD modified electrode;

[0040] 2) Immerse the 3D-G / HP-β-CD modified electrode in a solution containing 0.1M KCl, 5mM K 3 [Fe(CN) 6 ], 5mM K 4 [Fe(CN) 6 ] (pH 7.0), the electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) characterizations were carried out respectively (see Figure 4 , Figure 5 ), the 3D-G / HP-β-CD modified electrode exhibited low electrochemical impedance and high peak current;

[0041] 3) The 3D-G / HP-β-CD modified electrode was respectively immersed in a PBS buffer solution of 1 mM tryptophan at ...

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Abstract

The invention provides preparation of a three-dimensional graphene nano composite material and a method for chirally recognizing tryptophan (Trp). According to the preparation of the composite material, three-dimensional graphene (3D-G) is firstly synthesized by a microwave method; afterwards, the 3D-G and hydroxypropyl-beta-cyclodextrin (HP-beta-CD) are subjected to an esterification reaction tocarry out dehydration condensation to make the composite material (3D-G / HP-beta-CD). The composite material can be used for realizing the electrochemical recognition on a chiral molecule by means of achiral cavity structure of cyclodextrin and the excellent electrochemical performance of the 3D-G; a chiral recognition sensor is constructed. The tryptophan (Trp) is used as a chiral amino acid; twoenantiomers D-Trp and L-Trp of the Trp present obviously different chiral inclusion differences in the presence of the 3D-G / HP-beta-CD nano composite material and the invention provides the method for simply and quickly chirally recognizing the Trp by utilizing the chiral recognition effect of the 3D-G / HP-beta-CD.

Description

technical field [0001] The invention relates to the field of chiral recognition of nanomaterials, in particular to the preparation of a three-dimensional graphene nanocomposite material and a method for chiral recognition of tryptophan. Background technique [0002] As an essential amino acid for the human body, tryptophan has two enantiomers, D-type and L-type, and their physiological activities and pharmacological effects are significantly different. L-tryptophan can only be ingested from food. It can prevent depression, improve sleep and regulate mood, etc. It plays an indispensable role in the growth and metabolism of humans and animals. D-tryptophan has almost no metabolism in the human body, but it has nutritional value for some animals, and can be used as a raw material for the synthesis of sweeteners and plant auxins, and has special medicinal effects. Therefore, chiral recognition of tryptophan enantiomers becomes crucial. The research on chiral recognition of try...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/30
CPCG01N27/308G01N27/48
Inventor 梁文婷戎艳琴范丽芳董文娟马学文芦冬涛董川双少敏
Owner SHANXI UNIV
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