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Multipurpose culture vessel for assisting reproduction test

An assisted reproduction, petri dish technology, applied in tissue cell/virus culture devices, specific-purpose bioreactors/fermenters, biochemical instruments, etc. problem, to achieve the effect of improving growth rate, strong practicability and good use effect

Pending Publication Date: 2018-02-23
PEKING UNIV SHENZHEN HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] And for the traditional in vitro fertilization culture dish, it generally is to directly arrange the sperm placement cavity and the egg placement cavity in the corresponding culture dish, and connect a sperm guide channel horizontally in the middle part of the outer wall between them, so that the activity is good. The sperm can run to the egg placement cavity through the corresponding sperm guide channel and combine with the egg placed in it, so as to achieve the purpose of egg fertilization in vitro. Although this method is widely used, it still has certain defects and deficiencies.
[0004] Specifically, on the one hand, the sperm with good motility could not find the sperm-guiding channel, lost their way and remained in the corresponding sperm placement cavity, while the sperm with poor motility might run to the egg placement cavity through the corresponding sperm-guiding channel The combination with the eggs placed in it makes it impossible to efficiently ensure the combination of excellent sperm and eggs, which will affect the quality of the reproduced offspring, and it is more likely to reduce the development rate of fertilized embryos and the success rate of embryo transfer. On the one hand, most of the active sperm are suspended in the upper part of the corresponding sperm storage cavity, most of the high-quality eggs sink to the bottom of the corresponding egg storage cavity, and most of the poor quality eggs are suspended in the inner upper part of the corresponding egg storage cavity. The exit of the sperm guide channel is located in the middle of the cavity where the eggs are placed. In this way, there are also sperms with good or poor viability combined with eggs with poor quality, so that it is still impossible to efficiently ensure that the sperm with good vigor combine with high-quality eggs. Moreover, the existing petri dish for in vitro fertilization has a single function and cannot be used as a petri dish for assisted reproduction experiments, which makes the practicability not strong
[0005] Thereby, the existing in vitro fertilization culture dish needs to be improved

Method used

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Embodiment Construction

[0029] The technical solution of the present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, so as to understand the essence of the present invention more clearly and intuitively.

[0030] combine figure 1 and figure 2 shown;

[0031] A multi-purpose petri dish for assisted reproduction experiment provided by the present invention includes a petri dish 10 and a lid for covering the petri dish 10 .

[0032] The improvement of the present invention is that: the petri dish 10 includes a dish main body 101, an egg holding cup 102, a plurality of sperm holding cups 103 and a plurality of sperm passages 104;

[0033] Wherein, the inner middle part of the main body 101 of the dish is fixed with a columnar support 1011, and the egg placement cup 102 is fixed on the columnar support 1011; a plurality of the sperm storage cups 103 are placed around the egg. The cups 102 are evenly fixed in the main body 101 of the d...

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Abstract

The invention discloses a multipurpose culture vessel for assisting a reproduction test. The multipurpose culture vessel comprises a culture vessel and a vessel cover, wherein the culture vessel comprises a vessel main body, an ova placing cup, a plurality of sperm placing cups and a plurality of sperm channels; a columnar supporting platform is arranged in the middle of the vessel main body; theova placing cup is fixed on the columnar supporting platform; the sperm placing cups are all arranged in the vessel main body in a manner of surrounding the ova placing cup; the height of the rims ofthe sperm placing cups is slightly lower than that of the bottom of the ova placing cup; a sperm guide-out hole is formed in the outer wall of each of the sperm placing cups near the rim; a sperm guide-in hole is formed in the outer wall of each of the sperm placing cups near the bottom; each of the sperm channels is correspondingly fixedly between each of the sperm guide-out holes and each of thesperm guide-in holes that are opposite. The multipurpose culture vessel has the beneficial effects that the sperm channels are slantingly arranged, so that the survival of the fittest in moving of sperm can be reflected, and the sperm with best activity can only pass the sperm channels and move to the ova placing cup to be combined with high-quality ova, therefore, the multipurpose culture vesselis strong in practicability and good in using effect.

Description

technical field [0001] The invention relates to the technical field of medical equipment, in particular to a multipurpose petri dish for assisted reproduction experiments. Background technique [0002] A petri dish, as the name suggests, is a laboratory vessel used for microorganism or cell culture. A traditional petri dish consists of a disc-shaped main body and a cover, which are generally made of plastic or glass. Plastic is suitable for laboratory inoculation, streaking and isolation of bacteria, and can also be used for cultivation of plant materials. Glass can be used for plant material, microbial culture and animal cell adherent culture. [0003] And for the traditional in vitro fertilization culture dish, it generally is to directly arrange the sperm placement cavity and the egg placement cavity in the corresponding culture dish, and connect a sperm guide channel horizontally in the middle part of the outer wall between them, so that the activity is good. The sperm...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12M1/22C12M1/12C12M1/00
CPCC12M23/02C12M23/10C12M23/34C12M33/14
Inventor 周亮
Owner PEKING UNIV SHENZHEN HOSPITAL
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