Detection method for evaluating curative effect and residual condition of heparin drugs

Abstract

The invention relates to the field of blood detection, in particular to a detection method for evaluating the curative effect and the residual condition of heparin drugs. The heparin drugs mainly comprise heparin, low-molecular-weight heparin and some heparinoid drugs. The detection method mainly comprises steps as follows: heparinase, a freeze-drying protective agent and an adhesive are mixed, then a mixed reagent in appropriate proportion is prepared from an obtained mixture by the aid of a buffer solution, the mixed reagent is enveloped in a test-cup and subjected to freezing and vacuum pumping treatment, and a heparinase cup is obtained. The detection method has a plurality of advantages that the operation is simple, the guarantee period is long, the stability is good and the like. Theheparinase cup and a common cup (a blank sample cup) are put in a thrombelastogram, an anticoagulant and a chelating agent are added to the cups respectively, endogenously activated whole blood samples are added to the test-cups, and thromboelastography is started for detection; values R of starting time of blood clot formation in the heparinase cup and the common cup are compared, and whether heparin exists in the blood can be judged; if heparin exists, the value R of the starting time of blood clot formation in the heparinase cup is smaller than that of the starting time of blood clot formation in the common cup. The method is simple to operate, a detection result is good in repeatability, and the accuracy is high.

Description

technical field [0001] The invention relates to the technical field of blood detection, in particular to a detection method for assessing curative effect and residue of heparin, low molecular weight heparin and heparin-like drugs by thromboelastography. Background technique [0002] Heparanase refers to a type of polysaccharide lyase that can specifically cleave the glycosidic bonds of heparin and heparin-like main chains. It was first discovered and isolated from Flavobacterium heparinus, and later found in some microorganisms and animal tissues. Heparanase is present. At present, there are three kinds of heparanases that have been isolated and purified, namely heparanase I, II and III, and the enzymatic properties of these three enzymes are different. Among them, heparinase I is widely used, such as: removing residual heparin in blood, preventing blood coagulation, producing low molecular weight heparin, studying the structure of heparin, etc. In 1948, German Harter inve...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a detection method for detecting and evaluating the curative effect and residue of heparin, low molecular weight heparin and heparin-like drugs, so as to solve the problems of poor stability, short validity period and poor detection accuracy existing in the existing detection

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