Method for improving cell compatibility of bio-derived hydroxyapatite

Abstract

The invention discloses a method for improving the cell compatibility of bio-derived hydroxyapatite. The method comprises: after freezing pig limb bones for three months at a temperature of -176 to -18 DEG C, removing soft tissues from the surface of the bone tissue, sawing the bone into loose bone blocks by using a bone saw, carrying out ultrasonic cleaning with purified water without organic solvents, removing the bone marrow components, placing in a freeze-drying machine, and carrying out freeze-drying; placing in a supercritical carbon dioxide reaction kettle, carrying out a reaction, taking out, placing in an alkali solution, carrying out freeze-drying, taking out, placing in a ventilation muffle furnace, and carrying out gradual temperature increase and decrease treatment; and packaging, and carrying out cobalt-60 gamma ray radiation sterilization to prepare the bio-derived hydroxyapatite. According to the present invention, the degreasing and protein removing treatment is performed with supercritical carbon dioxide so as not to produce the organic matter residue, such that the biocompatibility and the biomechanical properties of the prepared material can be effectively improved.

Description

technical field [0001] The invention belongs to the technical field of biological transplantation materials, and in particular relates to a method for improving the cell compatibility of biologically derived hydroxyapatite. Background technique [0002] Bone transplantation is the second largest tissue transplant after blood transfusion. Currently, the commonly used bone graft materials mainly include bio-derived materials and artificial synthetic materials. Biologically-derived materials have a more similar physical structure and chemical structure to autologous bone tissue. The composition is widely used in clinic, but bone tissue derived from allogeneic or heterogeneous bone tissue has strong antigenicity, and it is easy to cause immune rejection after transplantation, resulting in transplantation failure. In order to effectively reduce the immunogenicity of such materials, often Decellularization, deproteinization and other methods are used to remove the antigen carrier,...

AI Technical Summary

Problems solved by technology

[0002] Bone transplantation is the second largest tissue transplant after blood transfusion. Currently, the commonly used bone graft materials mainly include bio-derived materials and artificial synthetic materials. Biologically-derived materials have a more similar physical structure and chemical structure to autologous bone tissue. The composition is widely used in clinic, but bone tissue derived from allogeneic or heterogeneous bone tissue has strong antigenicity, and it is easy to cause immune rejection after transplantation, resulting in transplantation failure. In order to effectively reduce the immunogenicity of such materials, often Decellularization, deproteinization and other methods are used to remove the antigen carrier, and the most commonly used method of deantigenization for xenograft bone is calcined bone prepared by high temperature calcination to remove collagen and other organic components in bone tissue, the main component of which is hydroxyl Apatite, this kind of bio-derived hydroxyapatite effectively reduces the antigenicity, but it loses the good cytocompatibility of the original bone tissue, which is not conducive to the attachment of osteoblasts after implantation, thus affecting the osteogenesis At the same time, it needs to use a large amount of organic solvent to remove protein during the preparation process, which is easy to affect the microstructure of bone tissue and cause organic solvent residue, which affects the biocompatibility of the material

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