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Method and application for solid state fermentation of ginkgo leaves by using eurotium cristatum

A technology of solid-state fermentation of S. coronis, which is applied in the fields of application, botany equipment and methods, and horticulture, and can solve the problem of low spore count of S. coronis, lack of fermentable carbon source, nitrogen source, and the lack of fermentable carbon and nitrogen sources. The problem of limited growth of cystic fungus can achieve the effect of benefiting absorption, improving antioxidant activity and improving feeding effect

Active Publication Date: 2017-12-19
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In view of the above research status, the current fermentation of Ginkgo leaves by S. coronoidis has the following problems: (1) In terms of fermentation target positioning, it is mainly to prepare ginkgo leaf tea or to obtain new biologically active substances through fermentation, and there is no sacs for coronoids. Bacteria fermented Ginkgo biloba as feed or feed additive for investigation; (2) In the existing reports, the fermentation medium only uses Ginkgo biloba powder as raw material configuration, lacks fermentable carbon source and nitrogen source, and the growth of S. coronoidis is limited, and finally The number of spores of S. coronis in the fermentation product is small; (3) There is no research on the growth and decline of digestive enzymes during the fermentation of Ginkgo leaves by S. coronis

Method used

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  • Method and application for solid state fermentation of ginkgo leaves by using eurotium cristatum
  • Method and application for solid state fermentation of ginkgo leaves by using eurotium cristatum
  • Method and application for solid state fermentation of ginkgo leaves by using eurotium cristatum

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preparation example Construction

[0031] Activation of S. coronis and preparation of spore suspension:

[0032] Separation and purification: Take out the strains stored at minus 20°C, and use a sterilized and cooled inoculation loop to pick up golden colonies with edges on the aseptic operating table, and inoculate freshly prepared PDA by streaking on a plate on the culture medium. 28 ℃, relative humidity 85%, constant temperature and humidity culture. After culturing until golden-yellow closure shells grow on the plate (about 72 hours), use a sterilized inoculation loop to pick out golden-yellow colonies at the edges, and inoculate the strains onto new PDA. Repeat the above operation 2 to 3 times.

[0033]Preparation of spore suspension: Put 20 to 30 glass beads in a Erlenmeyer flask filled with sterile water, pick PDA plates and culture them for 72 hours and move them into sterile water. It is 28°C, 220rpm, 45min. Spore counts were performed using a hemocytometer to adjust the concentration.

Embodiment 1

[0035] (1) Remove impurities from the freshly picked ginkgo leaves, wash them, dry them in an electric blast drying oven at 40°C for 6 hours, crush them with a pulverizer and sieve them to a size of 40 mesh to obtain ginkgo leaf powder, take 8.5g and place them in 100mL In the Erlenmeyer flask, the thickness is better to cover the bottom of the Erlenmeyer flask;

[0036] (2) Dry the ginkgo fruit in an electric blast drying oven at 60°C for 5 hours, pulverize and sieve the ginkgo fruit to a size of 40 mesh to obtain the whole powder of the ginkgo fruit. Add 0.675g of ginkgo fruit powder to a 100mL Erlenmeyer flask, then add 5g of phosphate buffer solution with a pH of 4.5, mix the ginkgo leaf powder, ginkgo fruit powder and buffer solution, seal with a sterile sealing film, and keep at 121°C High-temperature and high-pressure sterilization for 20 minutes to obtain the ginkgo leaf solid-state fermentation medium is cooled for subsequent use;

[0037] (3) Inoculate the spore sus...

Embodiment 2

[0039] (1) Remove impurities from the freshly picked ginkgo leaves, wash them, dry them in an electric blast drying oven at 40°C for 6 hours, crush them with a pulverizer and sieve them to a size of 40 mesh to obtain ginkgo leaf powder, take 8.0g and place them in 100mL In the Erlenmeyer flask, the thickness is better to cover the bottom of the Erlenmeyer flask;

[0040] (2) Dry the bean cake in an electric blast drying oven at 60°C for 5 hours, pulverize and sieve the bean cake to a size of 40 mesh to obtain bean cake powder. Add 0.735g of bean cake powder to a 100mL Erlenmeyer flask, then add 2.5g of phosphate buffer solution with a pH of 4.0, mix the ginkgo biloba powder, bean cake powder and buffer, seal with sterile parafilm, and extinguish at 121°C under high temperature and high pressure. Bacteria 20min, get the ginkgo leaf solid-state fermentation medium after cooling and set aside;

[0041] (3) Inoculate the spore suspension of Saccharomyces coronoidis in the solid-s...

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Abstract

The invention discloses a method and application for solid state fermentation of ginkgo leaves by using eurotium cristatum. The method comprises the following steps that 1, the ginkgo leaves are smashed, and ginkgo leaf powder is obtained and placed in a container; 2, minor ingredients are added sequentially, then a buffer solution is added into the minor ingredients, evenly mixed and sterilized, and a ginkgo leaf solid state fermentation culture medium is obtained; 3, an eurotium cristatum spore suspension is inoculated into the solid state fermentation culture medium for fermentation, and the fermented ginkgo leaves are obtained. According to the method, the ginkgo leaves are subjected to fermentation by using the eurotium cristatum, all the nutrient and function components of the ginkgo leaves are increased, and the content of harmful components is reduced; meanwhile the fermented ginkgo leaves have rich eurotium cristatum spores and various digestive enzymes; in addition, through degradation of pectin, cellulose and lignin, the taste of the fermented ginkgo leaves is delicate, the fermented ginkgo leaves are used as feed or added into the feed, and the palatability of the feed is improved; the method is beneficial for absorption of the feed by animals, and the feeding effect is improved. Therefore, the eurotium cristatum solid state fermentation ginkgo leaves are expected to be novel biological fermentation feed and feed additives.

Description

technical field [0001] The invention relates to the field of biological fermentation, in particular to a method for solid-state fermentation of Ginkgo biloba leaves by Saccharomyces coronoides and an application thereof. Background technique [0002] Cystis coronis, commonly known as "Golden Flower" fungus, belongs to a fungus belonging to the order Phytomycetes, Phytomycetaceae, and Phytocystis genus, and its body is golden yellow. During the process of "blossoming" Fuzhuan tea, S. coronoidis is the dominant bacterium throughout the whole process. The unique quality and unique flavor of Fuzhuan tea is due to the fungus S. coronoidis. According to the national standard, Fuzhuan tea is also the only tea variety with the index of "Coronoid Saccharomyces" among the dark tea varieties. The research on A. coronis began in the 1950s. Initially, A. coronis was considered as a yellow mold, and later it was identified as Aspergillus shevai. Due to the limited research conditions at...

Claims

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Application Information

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IPC IPC(8): A01G1/04A23K10/00A23K10/10
CPCA23K10/00A23K10/10A01G18/00Y02P60/87
Inventor 苏二正徐瑾曹福亮汪贵斌赵林果祝遵凌黄彦
Owner NANJING FORESTRY UNIV
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