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Method for mycoplasm culture of Mili entomogenous fungus for highly yielding carotenoids, and preparation methods of Mili entomogenous fungus products

A technology of carotene and culture method, which is applied in the high-yielding carotenoid-producing mycoplasma culture method and its products, to achieve the effects of increasing carotenoid content, optimizing culture conditions, and optimizing induction conditions

Active Publication Date: 2017-12-15
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Nowadays, the research on carotenoids contained in Myrrhia has been increasing, but most of them are still in the stage of extraction and identification, and there are few reports on how to increase the content of carotenoids

Method used

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  • Method for mycoplasm culture of Mili entomogenous fungus for highly yielding carotenoids, and preparation methods of Mili entomogenous fungus products
  • Method for mycoplasm culture of Mili entomogenous fungus for highly yielding carotenoids, and preparation methods of Mili entomogenous fungus products
  • Method for mycoplasm culture of Mili entomogenous fungus for highly yielding carotenoids, and preparation methods of Mili entomogenous fungus products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Example 1 Optimization of bacterial culture conditions

[0088] Inoculate the original strains stored in the slant or strain tube on the PDA solid plate medium, inoculate 3 times for each type of seed preservation bacteria as repeats, cultivate at 25°C under constant temperature and shade until the hyphae cover the petri dish, and then turn the color of the bacteria under light. , to obtain activated strains.

[0089] Take a 250-mL conical flask, add 100 mL of PDA liquid culture medium to each bottle, seal the bottle, and sterilize at 121 °C for 30 min. On the activated plate covered with yellow mycelium, take 3 to 4 pieces of mycelium pieces the size of soybean grains and inoculate them into the prepared PDA liquid medium. After inoculation, place it in a shaker at 25°C and 150r / min for 4 to 5 days of shaking culture.

[0090] Mycoplasma culture: Weigh the ingredients in each bottle according to the culture material formula, add liquid and stir well. The bottle was ...

Embodiment 2

[0113] Example 2 Optimization of Chitosan Induction Conditions

[0114] When the mycelium of the Mycobacterium is covered with light, the concentration of the inducer should be scientifically and reasonably designed, and the sterilized 2mg / mL, 1mg / mL, 0.5mg / mL and 0.05mg / mL chitosan should be sprayed respectively. 2 mL of each concentration was sprayed to measure the carotenoid content. The results are shown in Table 2. The results show that when the concentration of chitosan is 2 mg / mL, it is significantly different from the others, and has the greatest impact on the carotenoid production of Myriales, which is 15% higher than that of the control group, indicating that chitosan can induce Mysticobacteria produce carotenoids.

[0115] Table 2 The effect of chitosan on the production of carotenoids by Myriales

[0116]

Embodiment 3

[0117] The preparation of embodiment 3 Miriameria carotenoid powder and nutrient composition determination

[0118] After the fermentation, the fresh Mycobacterium culture medium produced in Example 1 was placed in a 55° C. oven to dry, pulverized and passed through a 70-mesh sieve to obtain Mycobacterium carotenoid powder. Then each active ingredient was measured to evaluate its nutritional value. Commercially available fruiting bodies were used as controls.

[0119] 1. Analysis and determination of polysaccharides

[0120] (1) Take a certain amount of sample, the ratio of material to liquid is 1:30 (w / v), add distilled water, extract by ultrasonic wave (400W, 5min), filter to obtain supernatant, concentrate the filtrate under reduced pressure to a certain volume, add 4 times 95% ethanol by volume and placed in a 4°C refrigerator overnight. The next day, centrifugation (4200r / min, 10min) polysaccharide precipitation, reconstituted with water to obtain Cordyceps basal crude...

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Abstract

The invention discloses a method for mycoplasm culture of a Mili entomogenous fungus for highly yielding carotenoids, products thereof, and preparation methods of the products. Three optimum edible media are obtained through researches and summary by creatively adopting the carotene content of the Mili entomogenous fungus as an index, and optimum culture conditions obtained through the researches and summary are as follows: the inoculation amount of a mycoplasm culture medium is 25%, the dark culture is carried out at 22-28 DEG C for 11-13 days, and illumination culture is carried out at 25 DEG C for 25-45 days. Chitosan used as an inducer for the first time further makes carotenoid yield of the Mili entomogenous fungus be highest and keep stable. The culture method has the advantages of simplicity in operation, energy saving, remarkable improvement of the yield of the carotenoids in the Mili entomogenous fungus, and wide market application prospect. The invention also provides three carotenoid-rich Mili entomogenous fungus products prepared from a mixed matrix of the media and the Mili entomogenous fungus produced through the mycoplasm culture method for improving the carotenoid content of the Mili entomogenous fungus, and preparation methods thereof.

Description

technical field [0001] The invention belongs to the field of artificial cultivation of mycobacterium, and more particularly relates to a method for culturing mycoplasma of mycobacterium with high carotenoid yield and a product thereof. Background technique [0002] In traditional Chinese medicine, some Cordyceps fungi, such as Cordyceps sinensis, Myriads, and Cordyceps grandis, can often be directly used as medicine in their complexes with their hosts. [0003] Mystia is an entomogenous fungus with very similar medicinal value to Cordyceps sinensis, containing biologically active substances such as cordycepin acid, cordycepin, cordyceps polysaccharide, steroids, mannitol and superoxide dismutase (SOD). Myriaderia also contains 17 kinds of amino acids, and the total protein content is as high as 30%, of which 35.47% are essential amino acids. In addition, myriad bacteria also contain V A , V D , V E , V C , V B1 , V B2 and V B6 and other vitamins. In addition, Cordyc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P23/00C12R1/645
CPCC12N1/14C12P23/00
Inventor 林俊芳郭丽琼刘聪简锦辉云帆叶志伟康林芝殷林
Owner SOUTH CHINA AGRI UNIV
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