Equipment for monitoring bacterium growth and monitoring method

Abstract

The invention provides equipment for monitoring bacterium growth and a monitoring method. The equipment comprises a constant-temperature incubator, scanners, a computer and a bacterium growth monitoring processing system which is arranged in a CPU of the computer, wherein the plurality of scanners, which are connected to the computer by virtue of data lines and concentrators, are arranged in the constant-temperature incubator, and a plurality of culture dishes are arranged in each scanner; and the bacterium growth monitoring processing system, which is arranged in the CPU of the computer is composed of an image acquisition module, an image processing module, an image analysis module and a result representation module. According to the monitoring method provided by the invention, the consistency of colony size and a pixel area can be obtained by virtue of the scanners, so that the situation on bacterium growth can be obtained; the method, in comparison with a conventional method which adopts a spectrophotometer to measure a bacterium amount, can greatly reduce system errors and accidental errors; the monitoring method provided by the invention is applicable to bacterium counting as well as bacterium separation and identification, and the monitoring method is also applicable to mixed sample detection, high-throughput screening of plasmid inhibitors and the like; and in addition, a function of microbial imaging is achieved.

Description

technical field [0001] The invention belongs to the technical field of biochemistry and relates to a bacteria monitoring technology, in particular to a device and a monitoring method for monitoring the growth of bacteria. Background technique [0002] Mastering the growth law and isolation and identification methods of bacteria has important guiding significance for the study of bacterial physiology and production practice. For example, in production, select appropriate strains, bacterial ages, and culture media to shorten the lag period; in the preparation of sterile preparations and infusions, the sterilization process must be arranged in the lag period to reduce pyrogen contamination; work in the laboratory In the process, bacteria in the logarithmic phase should be used as experimental materials as much as possible; in the fermentation industry, in order to obtain more metabolites, the stable period can be properly adjusted and extended; the spores mature in the recessio...

AI Technical Summary

Problems solved by technology

Although the method is accurate and reliable, the selection medium cannot identify and select the same kind of bacteria, and PCR technology requires a lot of consumables and a large workload

In terms of bacterial counting, if there are about 200 CFU colonies on each medium, and it is necessary to count the colonies on multiple mediums at the same time, manual counting is required, which is prone to errors

Images