Ethanol extract of paris dulongensis H. Li&Kurita, application of ethanol extract to pharmacy and separation and identification method of chemical components of ethanol extract
A technology for ethanol extraction and re-building, applied in chemical instruments and methods, plant/algae/fungus/moss components, material separation, etc.
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Embodiment 1
[0022] The preparation of PDE4 of the present invention and PDE5 and the separation of contained chemical composition thereof:
[0023] 1 kg of dried rhizome of Dulong Zhonglou, crushed, extracted with 3-4 liters of 90%-95% ethanol at 60-65°C for 3-4 hours, filtered to obtain filtrate and filter residue, and the filter residue was extracted twice according to the above conditions. Combine the filtrates obtained from the three extractions, and concentrate to obtain 133 g of the ethanol extract of Dulong Chonglou (referred to as PDE4).
[0024] Add 1 liter of water to PDE4 to make a suspension, and extract 3 times with 1 liter of petroleum ether, chloroform, and n-butanol successively. After the extract was concentrated, petroleum ether extract (2 g), chloroform extract (30 g) and n-butanol extract (30 g) were obtained respectively. The n-butanol extraction part is the total saponins of Dulong Chonglou, which is recorded as PDE5.
[0025] Segment PDE5 (30g) with 80-100 mesh si...
Embodiment 2
[0030] Structural identification of the chemical components isolated from the total saponins (PDE5) of the present invention:
[0031] Pachyphyll saponin V (PDE10), white solid, molecular formula C 39 h 62 o 12 , CAS registry number 19057-67-1. 1 H NMR (C 5 D. 5 N, 500MHz) δ: 6.40 (1H, br s), 5.29 (1H, br d, J = 5.1Hz), 1.78 (3H, d, J = 6.3Hz), 1.12 (3H, d, J = 7.0Hz) , 1.04 (3H, s), 0.81 (3H, s), 0.68 (3H, d, J=5.5Hz). 13 C NMR (C 5 D. 5 N, 126MHz) δ: 140.9, 121.8, 109.3, 102.2, 100.4, 81.1, 79.7, 78.4, 77.9, 77.8, 74.2, 72.9, 72.7, 71.8, 69.6, 66.9, 62.9, 62.7, 56.6, 50.3, 42.0, 40.5, 39.9, 39.0, 37.5, 37.2, 32.3, 32.3, 31.8, 31.7, 30.6, 30.2, 29.3, 21.1, 19.5, 18.8, 17.4, 16.4, 15.1. ESIMS m / z 721[M-H] - . Its NMR data are basically consistent with the data in the literature [Li Zhongqiong, Lin Ruichao, Fu Wen, Wang Gangli, Wei Feng, Hang Taijun. Research on the Chemical Components of Lettuce Flowers. Chinese Herbal Medicine, 2001,32(2):101-104].
[0032] Papaya...
Embodiment 3
[0038] The inhibitory effect of ethanol extract (PDE4), n-butanol extraction fraction (PDE5) and compounds isolated from PDE5 on the growth of cancer cells:
[0039] PDE4, PDE5 and the compounds PDE10, PDE11, PDE12, PDE14, PDE15 and PDE22 isolated from PDE5 were tested for their cytotoxic activity, and the results are shown in Table 1. The results showed that PDE4, PDE5, PDE10, PDE11, PDE14 and PDE15 had significant inhibitory activity on leukemia HL-60 cells, liver cancer SMMC-7721 cells, lung cancer A-549 cells, breast cancer MCF-7 cells and colon cancer SW480 cells, Its activity is comparable to the positive control drug cisplatin.
[0040] Table 1 Inhibitory effect of Dulong Chonglou extract and compounds on cancer cell growth
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