Serum-free culture medium for BHK cells

Abstract

The present invention provides a serum-free culture medium for BHK cells, wherein specifically the culture medium comprises amino acids, vitamins, salts, lipids, trace elements, a buffer agent, protein hydrolysates, acid-alkali indicators, and an additive. According to the present invention, with the culture medium, the separation and purification of the product is easily achieved, the quality of the product can be improved, the long-term BHK cell subculture can be supported, the long-term and complex adaptation process is not required, the BHK cell suspension growth can be supported, the preparation and the use of the serum-free culture medium are easy, and the serum-free culture medium is suitable for the large-scale production of biological products.

Description

technical field [0001] The invention relates to the field of cell culture, more specifically, the invention relates to a serum-free medium for BHK21 cell culture and a corresponding culture method. Background technique [0002] Foot-and-mouth disease is a zoonotic disease and a highly contagious viral disease. It is listed as the top animal disease epidemic in the world by the International Veterinary Bureau. Once foot-and-mouth disease breaks out, it can quickly evolve into a biological disaster, and the animal husbandry and related industries will be hit directly and even destructively. A large number of animals will be culled and destroyed, causing serious public health problems and social panic. From 1999 to 2001, foot-and-mouth disease swept the world. More than 50 countries in Asia, Africa, and Europe broke out foot-and-mouth disease one after another. Countries such as Britain, South Korea, and Japan, which had been free of the disease for many years, also fell ill, w...

AI Technical Summary

Problems solved by technology

As an important substance for cell growth and maintenance, serum plays an important role in cell culture, but it also has many problems and drawbacks that cannot be solved so far, such as: (1) The components in serum are complex and cannot be accurately distinguished; (2) There are great differences between serum batches, and the storage period of serum is short (no more than 1 year), which limits the standardization and serialization of batch production; (3) The serum may carry animal-derived viruses, mycoplasma, chlamydia, etc. (According to statistics, 20-50% fetal bovine serum is positive for virus detection), thereby polluting the cell culture process, making the safety of production questioned; (4) serum protein content is extremely high (protein content exceeds 45g / L) , which will bring great trouble to the subsequent separation and purification of the target protein and increase the cost

Images