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Extraction method for cistanche based phenylethanoid glycoside substance and anti-depression application thereof

A Cistanche extract and the technology of Cistanche, applied in the field of biomedicine, can solve problems affecting drug compliance, etc., achieve significant antidepressant activity, low cost, and easy industrial production

Inactive Publication Date: 2017-11-28
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to reports, almost all antidepressants can cause adverse reactions of sexual dysfunction, and nearly 36% of patients with depression regard sexual dysfunction caused by antidepressants as an unacceptable adverse reaction, which seriously affects the drug. compliance

Method used

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  • Extraction method for cistanche based phenylethanoid glycoside substance and anti-depression application thereof
  • Extraction method for cistanche based phenylethanoid glycoside substance and anti-depression application thereof
  • Extraction method for cistanche based phenylethanoid glycoside substance and anti-depression application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Cistanche deserticola and the preparation technology of each part thereof are as follows

[0033] Cistanche deserticola or Cistanche tubulosa medicinal material is crushed to obtain medicinal material powder; then the medicinal material powder is reflux extracted three times with 10 times the weight of 70% ethanol solution, each time for 2 hours, filtered with three layers of gauze, combined three times with the medicinal solution, and concentrated under reduced pressure to obtain Concentrate. The concentrated solution is separated by macroporous adsorption resin column chromatography, firstly eluted with water to obtain the water eluted part, then eluted with 40% ethanol solution, concentrated each part, and freeze-dried to obtain the water part and 40% ethanol elution site.

Embodiment 2

[0035] Antidepressant pharmacodynamics study of Cistanche deserticola and Cistanche tubulosa and their parts on mouse behavioral hopelessness model.

[0036] The mouse behavioral despair model (mouse tail suspension test, mouse forced swimming test) was used to evaluate the antidepressant effect of the alcohol extracts of Cistanche deserticola and Cistanche tubulosa and their different eluted parts.

[0037] Experimental animals: 135 male ICR mice, weighing 18g-20g.

[0038] Experimental group:

[0039] Experiment 1 group: blank group

[0040] Experimental group 2: 15mg / kg fluoxetine (positive drug)

[0041] Experimental group 3: 15mg / kg imipramine (positive drug)

[0042] Experimental group 4: 2181mg / kg desert Cistanche alcoholic extract

[0043] Experimental group 5: 1112mg / kg Desert Cistanche ethanol extract water elution part

[0044] Experimental group 6: 40% ethanol elution site of 917mg / kg desert cistanche alcoholic extract

[0045] Experimental group 7: 1555mg / kg...

Embodiment 3

[0065] Pharmacodynamic study of extracts from Cistanche cistanche on chronic unpredictable stimuli-induced depression in rat models

[0066] Establish a rat model of depression induced by chronic unpredictable stimuli, and use forced swimming test, sucrose preference test, open field test, and new environment feeding inhibition test to evaluate the antidepressant effect of 40% ethanol-eluted part of Cistanche cistanche ethanol extract effect.

[0067] Experimental animals: 40 male SD rats, weighing 180g-220g.

[0068] Experimental group:

[0069] Experiment 1 group: blank group

[0070] Experiment 2 group: model group

[0071] Experimental group 3: 10mg / kg fluoxetine (positive drug)

[0072] Experiment 4 group: 400mg / kg Cistanche tuberosum alcohol extract 40% ethanol elution site high dose group

[0073] Experimental group 5: 200mg / kg Cistanche cistanche ethanol extract 40% ethanol elution site low dose group

[0074] Experimental method: A total of 40 healthy male clean...

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Abstract

An application based on the extract of Cistanche deserticola, which is used to prepare antidepressant drugs; the extract of Cistanche deserticola is obtained by extracting the herb powder of Cistanche deserticola through liquid reflux to obtain a concentrated solution, and finally separating it by macroporous adsorption resin column chromatography effective part. The effective components prepared by using desert cistanche and cistanche tubulosum through a certain extraction and separation method have good antidepressant effect and can be applied to the preparation and development of antidepressant preparations.

Description

technical field [0001] The invention relates to a technology in the field of biomedicine, in particular to a method for extracting phenylethanol glycosides based on Cistanche deserticola and its antidepressant application. Background technique [0002] Modern pathological studies have shown that because the pathogenesis of depression is complex and there are many causes, it is often difficult to achieve satisfactory curative effect with drugs targeting a single link. Currently, synthetic drugs for the treatment of depression are mainly monoamine oxidase inhibitors, tricyclic antidepressants and serotonin reuptake inhibitors. Although synthetic antidepressants have certain effects, they generally have disadvantages such as narrow antidepressant spectrum, drug resistance, and large toxic and side effects. For example, about 12-15% of patients with depression do not improve their condition after taking antidepressant drugs designed based on the "monoamine neurotransmitter hypo...

Claims

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Application Information

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IPC IPC(8): A61K36/64A61P25/24A61K31/7032
CPCA61K36/64A61K31/7032A61K2236/331A61K2236/333A61K2236/55
Inventor 李晓波李瑒彭颖郑亮
Owner SHANGHAI JIAO TONG UNIV
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