Method for extracting arteannuin B from herba artemisiae annuae

Abstract

The invention discloses a method for extracting arteannuin B from herba artemisiae annuae. The method comprises the following steps: after crushing the herba artemisiae annuae, immersing the herba artemisiae annuae with methanol to obtain an immersing solution; then carrying out crude separation through a macroporous resin column, carrying out secondary separation through a polyamide adsorption resin chromatographic column, carrying out sephadex chromatographic column separation and carrying out high performance liquid chromatographic column separation in sequence. The method disclosed by the invention has a short separation period and used solvents only comprise methanol and pure water; most of solvents and separation columns can be repeatedly utilized so that the separation cost is saved; meanwhile, the method is safe to operate and is environmentally friendly. Meanwhile, the purity of the separated arteannuin B can reach 98 percent or more and scopoletin impurities can be removed. By adopting the method disclosed by the invention, the arteannuin B with high purity is effectively extracted from the herba artemisiae annuae and the extraction efficiency is high, so that the method is suitable for large-scale production and has very strong industrial practicability.

Description

technical field [0001] The invention relates to the field of preparation of natural medicinal chemistry, in particular to a method for extracting artemisinin from Artemisia annua. Background technique [0002] Artemisinin B, also known as artemisinin B, is a white powder, easily soluble in chloroform, dichloromethane, ethyl acetate and other solutions, and its molecular formula is C 15 h 20 o 3 , with a molecular weight of 248.3 and a CAS number of 50906-56-4, which is a sesquiterpene lactone compound that co-exists with artemisinin in the traditional Chinese medicine Artemisia annua. Artemisinin is a highly effective antimalarial drug, but artemisinin B showed a low antimalarial effect in drug screening against malaria in mice. However, in the Artemisia annua produced in some areas of my country, the content of artemisinin B is more than twice that of artemisinin. [0003] In recent years, many documents have indicated that artemisinin, artemisinic acid, and scopoletin ...

AI Technical Summary

Problems solved by technology

[0004] At present, the separation and preparation of artemisinin from Artemisia annua in experiments mostly use silica gel column separation method, etc., which mainly use organic solvents (such as chloroform, acetone, petroleum ether, etc.) for separation, and it is difficult to realize industrial production

Moreover, the scopoletin impurity in Artemisia annua is similar to the polarity of artemisinin, so it is difficult to separate the two, and it is difficult to obtain artemisinin with high purity.

At present, in order to obtain artemisinin with a purity of more than 98%, artemisinin is generally synthesized directly through organic synthesis, but the synthesis method is relatively complicated, and the process is difficult to control and the cost is high

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