Culture medium, culture medium composition, and preparation methods of culture medium and culture medium composition

Abstract

The invention relates to a culture medium, a culture medium composition, and preparation methods of the culture medium and the culture medium composition. The culture medium composition comprises: a first component, wherein the first component is the structure support component of the culture medium composition, and is a gel; a second component, wherein the second component is the remaining component excluding the first component and a third component in the culture medium composition; and the third component, wherein the third component is the component unstable to room temperature, illumination or solutions, or complete animal living cells, or the component capable of producing denaturation during high temperature and high pressure sterilization, and is required to be separately stored and transported by considering the specific property of the component. According to the present invention, during the preparation of the culture medium, only the first component is required to be heated to dissolve the gel, and then the heated first component is mixed with the second component and the third component so as to complete the preparation of the culture medium; the preparation time before the experiment is saved, the method is suitable for industrial production, the standardization degree of the microbiological detection is improved, the solid mixing mode of the traditional dry powder culture medium is replaced with the liquid mixing mode, the components of the culture medium are uniform, and the inter-assay stability and the quality reliability of the microbial culture medium are improved.

Description

technical field [0001] The invention relates to the technical field of microorganisms, and more specifically relates to a medium, a medium composition and a preparation method thereof. Background technique [0002] Culture medium is artificially prepared and suitable for the nutritional basis of microbial growth, separation and identification. The general basal medium contains basic nutrients such as peptone, beef extract powder, yeast extract powder, sodium chloride, and agar. In the medium used in clinical and food hygiene inspection, it is often necessary to add bacteriostatic agents, indicators, blood, sugars and other components to facilitate the isolation and identification of specific bacteria, while the culture used in virus culture and vaccine production base, you need to add different kinds of amino acids, nucleotides, inorganic salts, growth factors and other substances. The complexity of the medium components and the difference in the stability of the additives...

AI Technical Summary

Problems solved by technology

The whole operation process takes about two to three hours, and the operation is cumbersome, resulting in a waste of human resources

[0013] (2) The component balance of dry powder medium is often not ideal

[0018] (3) The preparation process often needs to protect the stability and effectiveness of some ingredients by lowering the sterilization temperature or reducing the sterilization time, which leads to an increase in the probability of product contamination

[0019] For example, bismuth sulfite agar, because bismuth sulfite is unstable at high temperature, cannot be sterilized by autoclaving, and can only be boiled to kill microbial propagules, and in the state of boiling, fungal spores and bacterial spores cannot be killed of

[0020] (4) Since the melting temperature of agar is about 90-95°C, the usable temperature of the medium after liquefaction is usually 50°C, and the cooling time required after preparation is long

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