Method for treating complementary determining region (CDR) of T cell receptor (TCR) beta chain

Abstract

The invention relates to a method for treating a complementary determining region (CDR) of a T cell receptor (TCR) beta chain. The method comprises the following steps: setting an experimental group and a control group, wherein each group comprises a plurality of parts of peripheral blood samples which are dissimilar to a human body and get away from the human body; separating lymphocytes of the peripheral blood samples, and respectively extracting deoxyribonucleic acid (DNA) in the lymphocytes of the peripheral blood samples; adopting a multiplex polymerase chain reaction (PCR) technique to establish a DNA library; carrying out high-throughput sequencing on the DNA library, and carrying out data analysis on the CDRs of TCR beta chains in the experimental group and the control group. The method for treating the CDR of the TCR beta chain is reasonable and feasible in design, and can be used for comprehensively analyzing the dynamic change characteristics of a receptor repertoire of the CDRs of the TCR beta chains in bodies of patients with hepatitis B virus-related cirrhosis at the decompensatory stage before and after transplantation and understanding the immune status of the patients so as to guide medication during monitoring disease situation after transplantation, prevent postoperative rejection and infection and provide a basis for the pathogenesis of diseases.

Description

technical field [0001] The invention relates to a method for acquiring information of liver transplantation recipients during the perioperative period as an intermediate result, in particular to a processing method for the complementarity-determining region of the TCRβ chain. Background technique [0002] Liver cirrhosis (liver cirrhosis, LC) is a common chronic liver disease, which is caused by a single or many pathogenic factors that continuously act on the liver, causing diffuse damage to the liver parenchyma, which further causes a series of liver function damage, and finally develops into a cirrhosis. Outcomes of arterial hypertension or advanced liver disease. It is characterized by hepatocyte degeneration and necrosis, residual liver cell nodule regeneration and fibrous tissue regeneration, leading to liver deformation and hardening, which has exceeded the compensatory capacity of liver cells. Its main pathogenesis is liver fibrosis. Liver fibrosis is the result of ...

AI Technical Summary

Problems solved by technology

Most patients with hepatitis B and patients undergoing surgery after liver transplantation are treated with antiviral drugs and immunosuppressants, but long-term use of these drugs will reduce immunity, making patients more susceptible to infection by other pathogens. Drug resistance, once drug resistance occurs, the curative effect will be reduced, and even the disease will be exacerbated

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