Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lactobacillus Plantarum ZN-3 and application thereof

A technology of Lactobacillus plantarum, ZN-3, applied in the application, bacteria, microorganisms and other directions, can solve the problems of different, bacteriostatic effect is not reported, the characteristics of different strains are not the same, etc., to increase resistance, The effect of promoting the growth of livestock and poultry and promoting intestinal peristalsis

Active Publication Date: 2017-11-03
WUHAN KEYUAN BIOLOGICAL DEV CO LTD
View PDF4 Cites 30 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some studies have also found that the characteristics of different strains are not the same, and the effects of different strains of the same strain are not the same. For the antibacterial effect of Lactobacillus plantarum, the current main research is on E. coli and Salmonella, Streptococcus suis, pig The bacteriostatic effects of Erysipelothrix, Haemophilus parasuis, porcine infectious pleuropneumonia and Basilicum have not been reported yet

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus Plantarum ZN-3 and application thereof
  • Lactobacillus Plantarum ZN-3 and application thereof
  • Lactobacillus Plantarum ZN-3 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Isolation and identification of Lactobacillus plantarum ZN-3:

[0034] 1. Isolation of Lactobacillus plantarum strains

[0035] Take Chinese herbal medicine fermented feed from an ecological pig farm in Guangxi, weigh 10g of fermented feed, add it to 90ml sterile single distilled water, culture at 25°C, 200rpm shaker for 30min, and serially dilute 10 times to 10 -7 , select an appropriate dilution and spread it on the lactic acid bacteria selective medium MRS medium, culture anaerobically at 35°C for 48-72h, pick a single colony on the plate, streak and purify it on the MRS medium plate multiple times, and aerobically culture at 35°C cultured to obtain pure colonies. Colony morphology observation and Gram staining were performed on the purified strains, and Gram-positive bacilli were selected. The colony diameter of the bacterial strain of the present invention is 1-2mm on the MRS medium, milky white, convex, circular, smooth surface, and facultative anaerobic. The s...

Embodiment 2

[0043] Bacteriostatic properties of Lactobacillus plantarum ZN-3

[0044] Clinically isolated pathogenic strains were used as indicator bacteria, which were:

[0045] Streptococcus suis type 2 SC19, Haemophilus parasuis SH0165, Actinobacillus pleuropneumoniae serotype 7 GZ9903, Escherichia coli 62 # (Man Xiaoying, Wu Bin, etc. Isolation, identification and characterization of porcine enterohaemorrhagic Escherichia coli. Chinese Journal of Zoonoses, 2009,25(10):984-987.), Pasteurella multocida HN06 (Liu W etc, Complete genome sequence of Pasteruella multocida HN06, a toxigenic strain of serogroupD.journal of Bacteriology, 2012Jun, 194(12):3292-3293), Erysipelothrix suis CY0160 (Wang yaetc.iTRAQ-based quantitative proteomic analysis potential virulencefactors of Erysipelothrix rhuriopathiae.Journal of Proteomics 160(2017):28-37.), Staphylococcus aureus J9(Xu haiyang etc.Evaluation of a novel chimeric B cellepitope-based vaccine against mastitis induced by either Streptococcus a...

Embodiment 3

[0060] Comparison of antibacterial effects of Lactobacillus plantarum ZN-3 and antibiotics on Escherichia coli and Staphylococcus aureus

[0061] Staphylococcus aureus J9 (Gram-positive bacteria) and Escherichia coli 62 # (Gram-negative bacteria) was used as an indicator bacterium, and the difference in the bacteriostatic effect of Lactobacillus plantarum ZN-3 and antibiotics on Gram-positive and negative bacteria was compared. The antibiotics used were penicillin, ampicillin, ceftriaxone (junbizhi), amikacin, gentamicin, kanamycin, neomycin, doxycycline, ofloxacin, cyclo Profloxacin, polymyxin B. The drug-sensitive paper was purchased from Hangzhou Microbiology Reagent Co., Ltd. Use NA as the plate medium for the antibacterial experiment.

[0062] Pick a single colony of Lactobacillus plantarum (Lactobacillus plantarum) ZN-3 and inoculate it in MRS liquid medium, cultivate it at 100 rpm at 35°C for 24 hours, and use it as the fermentation medium for ZN-3 antibacterial expe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses Lactobacillus plantarum ZN-3 and application thereof. The Lactobacillus plantarum ZN-3 provided herein is collected in CCTCC (China Center for Type Culture Collection) under CCTCC NO: M 2017286. The Lactobacillus plantarum ZN-3 provided herein features acid tolerance and cholate tolerance, can be implanted to intestines via the stomach to inhibit zoonotic pathogens, including gram-negative bacteria that includes Escherichia coli, salmonella, Pasteurella multocida, Erysipelothrix rhuriopathiae, Haemophilus parasuis and porcine infectious pleuropneumonia, as well as gram-positive bacteria that include Streptococcus suis, Staphylococcus aureus, and Staphylococcus epidermidis. Compared with antibiotics, the Lactobacillus plantarum ZN-3 has good antibacterial effect for pathogenic bacteria having high drug tolerance.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a strain of Lactobacillus plantarum (Lactobacillus plantarum) ZN-3 isolated from Chinese herbal medicine fermented feed, and also relates to the effect of the Lactobacillus plantarum ZN-3 on pathogenic animal sources. The inhibitory effect of pathogenic bacteria, and the application of probiotics in livestock and poultry breeding as prevention and treatment of animal intestinal diseases. Background technique [0002] Feed antibiotics are an antibiotic drug that can protect animal health and improve the production performance of livestock and poultry. Antibiotics have been widely used as feed additives in the livestock and poultry industry. With the emergence of problems such as the impact on human health, many countries have restricted the use of antibiotics for animal feed additives, trying to avoid the harm of antibiotics for feeding, especially in the past decade, the restrictions...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A23K10/18A61K35/747A61P31/04A61P1/12A61P37/04C12R1/25
CPCA61K35/747A23K10/18C12N1/205C12R2001/25Y02A40/70
Inventor 金梅林杨丽康超滑亚锋王杨波马海军
Owner WUHAN KEYUAN BIOLOGICAL DEV CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com