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Yeast strain capable of degrading zearalenone and tameness and cultivation method of yeast strain

A technology of zearalenone and yeast strains, applied in the direction of using microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., can solve problems such as environmental pollution, achieve high safety, have genetic stability, Effects in simple steps

Active Publication Date: 2017-11-03
HENAN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Adsorbent adsorption is also a method that can effectively remove zearalenone pollution, but the adsorption method will also adsorb the nutrients in the food while adsorbing the toxin, and because the toxin has not been degraded, it will pollute the environment
Commonly used chemical methods for removing zearalenone include ozone treatment, hydrogen peroxide treatment, and sodium carbonate soaking, etc., but the addition of chemical reagents will introduce many uncertain factors, and the potential toxicity of new products needs to be further studied

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] This embodiment provides a yeast strain that degrades zearalenone, the yeast strain is Saccharomyces cerevisiae Saccharomyces cerevisiae , Deposit number: CGMCC No. 13752, deposited in the "Common Microorganism Center of China Microbiological Culture Collection Management Committee" on March 14, 2017. The specific address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing.

[0021] This embodiment also provides a method for domesticating and cultivating a yeast strain that degrades zearalenone, which specifically includes the following steps:

[0022] (1) First weigh 200 g of potatoes, wash and peel them, cut them into small pieces, boil them into a paste, filter through eight layers of gauze to obtain mashed potatoes, add 20 g of glucose to the mashed potatoes, and then Pack into Erlenmeyer flasks and sterilize at 115°C for 25 minutes to prepare PDA liquid medium;

[0023] Then divide the PDA liquid medium into 10 parts, inoculate Angel yeast powder into o...

Embodiment 2

[0034] This example provides a method for acclimating and cultivating a yeast strain that degrades zearalenone. The specific steps are roughly the same as those in Example 1, except that:

[0035] In the step (1), zearalenone methanol stock solution was added to the remaining 9 parts of the PDA liquid medium to obtain zearalenone concentrations of 3.0 μg / mL, 3.5 μg / mL, and 4.2 μg / mL, respectively. Nine kinds of acclimatization media with different zearalenone concentrations of μg / mL, 4.65 μg / mL, 5.1 μg / mL, 5.67 μg / mL, 6.2 μg / mL, 6.3 μg / mL and 6.7 μg / mL, and respectively Pack into 9 Erlenmeyer flasks;

[0036] The step (3) is: inoculate the cycle starting strain into the acclimatization medium with a zearalenone concentration of 3.0 μg / mL with an inoculum size of 3% and place it at 30 °C, 180 rpm / After culturing on a shaking table for 72 hours at 1 min, the second-generation cycle starting strain was obtained, and the second-generation cycle starting strain was inoculated in...

Embodiment 3

[0039] This example provides a method for acclimating and cultivating a yeast strain that degrades zearalenone. The specific steps are roughly the same as those in Example 1, except that:

[0040] In the step (1), the PDA liquid medium was divided into 6 parts, and Angel yeast powder was inoculated into one part of the PDA liquid medium with a standard inoculum size of 3%, and incubated at 30°C , 180 rpm / min, and shake it at constant temperature for 48 hours to obtain angel yeast seed liquid. Add zearalenone methanol stock solution to the remaining 5 parts of the PDA liquid medium respectively to obtain zearalenone concentrations of 3.9 μg / mL, 4.82 μg / mL, 4.98 μg / mL, and 5.35 μg / mL, respectively. mL, 5.88 μg / mL of 5 kinds of acclimatization media with different zearalenone concentrations and put them into 5 Erlenmeyer flasks respectively to prepare acclimatization media;

[0041] The step (3) is: inoculate the cycle starting strain into the acclimatization medium with a zeara...

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PUM

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Abstract

The invention provides a tameness and cultivation method of a yeast strain capable of degrading zearalenone. The tameness and cultivation method comprises the following steps: taking angel yeast as a basic strain; inoculating angel yeast in a PDA culture solution which contains zearalenone with different concentrations; and carrying out step-by-step subculture to obtain a new yeast strain. The new yeast strain has been deposited under the accession number of CGMCC No. 13752. The new yeast strain has high safety, and can be used for degrading zearalenone toxin in feedstuff, products thereof, grains and products thereof into non-toxic products. Subculture of the new yeast strain is not degraded, and the new yeast strain has hereditary stability. Meanwhile, the tameness and cultivation method of the yeast strain has simple steps and is easy to operate.

Description

technical field [0001] The invention belongs to a method for biodegrading toxins, and in particular relates to a yeast strain for degrading zearalenone and a domestication and cultivation method thereof. Background technique [0002] Zearalenone (Zearalenone, ZEN) is a mycotoxin produced by a variety of Fusarium species and released into the environment. It has estrogen-like effects, mainly manifested as chronic toxicity, including toxicity to the reproductive system and genotoxicity and endocrine system Impact. Animals eating zearalenone-contaminated feed can easily lead to animal reproductive cycle disorder, and even cause reproductive disorders, affecting the body of animal production and development, especially in the fetus and neonatal period, some protective barriers in the body and metabolism, excretion And other physiological functions are not yet perfect, and they are more susceptible to zearalenone. Zearalenone can stimulate estrogen receptor transcription at 1-3...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/18C12N1/36C12R1/865
CPCC12N1/36C12N1/185C12R2001/865
Inventor 王金荣赵银丽李溪苏兰利
Owner HENAN UNIVERSITY OF TECHNOLOGY
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