A compound micro-ecological preparation for degrading nitrite in aquaculture ponds and its application
A technology of compound microecology and preparation, applied in the field of aquaculture, can solve the problems of nitrite accumulation and bacterial diseases
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Embodiment 1
[0032] Example 1: Preparation of Pseudomonas Fredericksburg Powder
[0033] (1) Seed tank culture: prepare a sterile triangular flask with glass beads inside, scrape and wash the mature bacterial slurry on the inclined surface of the eggplant bottle with sterile water, put it into the triangular flask, and shake and disperse the bacterial slurry to obtain a uniform bacterial suspension. 2%-10% by volume of the inoculum into the 100L seed tank, the filling coefficient of the seed tank is 60%-70% (v / v), that is, 60-70L of 100L seed canning medium.
[0034] The cultivation conditions of the seed tank are as follows: the stirring speed is 200r / min, the ventilation rate is 1-3m3 / h, the cultivation temperature is 25℃, and the cultivation time is 20-48h.
[0035] Seed tank medium: glucose 6g / L, yeast powder 12g / L, MgSO4.7H2O 3.5g / L, KH2PO4 2.5g / L, CaCl2 1.5g / L, pH7.0-7.2.
[0036] (2) Fermentation culture: the seed culture liquid is connected to the 1T fermenter with the inoculum vo...
Embodiment 2
[0048] Embodiment 2: the preparation of bacillus subtilis powder
[0049] (1) Strain activation: Aseptically open the freeze-dried and preserved strain of Bacillus subtilis, streak and inoculate it on the slant of nutrient agar test tube, cultivate it at 30-37°C for 24-48h, and then transfer it to the slant of nutrient agar eggplant bottle by streaking , Culture at 30-37°C for 24-48h; microscopic examination, when more than 90% of the bacteria form spores, it is mature.
[0050] Slant medium composition in g / L: peptone 10, beef extract 3, NaCl 5, agar 15-20, prepared with distilled water to constant volume, pH 7.0-7.2;
[0051] Seed tank cultivation: Prepare a sterile triangular flask with glass beads inside, scrape and wash the mature fungus sludge on the slope of the eggplant bottle with sterile water, put it into the triangular flask, oscillate to disperse the fungus slime, and obtain a uniform bacterial suspension. Heat the bacterial suspension in a water bath at 80°C for...
Embodiment 3
[0056] Embodiment 3: the preparation of bacillus amyloliquefaciens powder
[0057] (1) Strain activation: Aseptically open the freeze-dried and preserved strain of Bacillus amyloliquefaciens JSSW-LA, streak and inoculate it on the slant of nutrient agar test tube, culture it at 30-37°C for 24-48h, and then transfer the streak to the nutrient On the slant of agar eggplant bottle, culture at 30-37°C for 24-48h; microscopic examination, when more than 90% of the bacteria form spores, it is mature.
[0058] Slant medium composition in g / L: peptone 10, beef extract 3, NaCl 5, agar 15-20, prepared with distilled water to constant volume, pH 7.0-7.2;
[0059] (2) Seed tank cultivation: Prepare a sterile triangular flask with glass beads inside, scrape and wash the mature fungal slime on the slope of the eggplant bottle with sterile water, put it into the triangular flask, oscillate and disperse the fungal slime, and obtain a uniform bacterial suspension liquid. Heat the bacterial s...
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