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Staphylococcus haemolyticus applied to preventing and treating xanthomonas oryzae pv. oryzae and application thereof

Active Publication Date: 2017-09-26
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the control methods of rice bacterial blight mainly include strengthening field management, cultivating disease-resistant varieties, using chemical agents, and applying biocontrol agents, etc., but practice has proved that field management is difficult to effectively control the occurrence of rice bacterial blight. The breeding of non-toxic materials takes a long time, while chemical control pollutes the environment and destroys the ecological balance, and the long-term use of chemical agents can easily cause pathogenic bacteria to develop drug resistance

Method used

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  • Staphylococcus haemolyticus applied to preventing and treating xanthomonas oryzae pv. oryzae and application thereof
  • Staphylococcus haemolyticus applied to preventing and treating xanthomonas oryzae pv. oryzae and application thereof
  • Staphylococcus haemolyticus applied to preventing and treating xanthomonas oryzae pv. oryzae and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: Bacteria is carried out separation and purification:

[0016] 1. Medium

[0017] LB medium: 10g of Tryptone, 5g of Yeast extract, 10g of NaCl, 15g of agar, H 2 Dilute to 1000ml with O, pH 7.0-7.2. Sterilize at 121°C for 20 minutes.

[0018] 2. Experimental steps

[0019] 2.1 Culture medium preparation

[0020] Prepare the LB agar medium as described in 1, which is a bacterial culture medium.

[0021] 2.2 Sample collection

[0022] Select a representative location (the paddy field in Yongzhou, Hunan Province), shovel off the surface soil, and use soil mining tools to collect soil in the soil layer at a depth of 5 to 10 cm below the surface. Take 5 samples from each location and put them into bags and make a record.

[0023] 2.3 Preparation of soil dilution

[0024] Weigh 10g of soil, put it into a Erlenmeyer flask filled with 90mL of sterile water and shake it fully to make a soil dilution with a concentration of 1:10 (volume ratio). After the soil ...

Embodiment 2

[0029] Embodiment 2: the plate antagonism test of isolated bacteria to Xanthomonas oryzae

[0030] 1. Medium

[0031] 1.1 Solid medium

[0032] LB medium: 10g of Tryptone, 5g of Yeast extract, 10g of NaCl, 15g of agar, H 2 Dilute to 1000ml with O, pH 7.0-7.2. Sterilize at 121°C for 20 minutes.

[0033] 1.2 Liquid medium

[0034] LB liquid medium: without adding agar.

[0035] 2. Experimental steps

[0036] 2.1 Plate Antagonism Test ----- Primary Screening

[0037] 2.1.1 Strain activation

[0038] Strain activation of bacterial blight of rice and bacteria: bacterial blight of rice PXO99 (Philippine race 6 of bacterial blight of rice, purchased from ATCC) and isolated bacteria were transferred to LB medium plates, respectively, at 28°C and 30°C, cultured for 2 days and 1 day, and set aside.

[0039] 2.1.2 Preparation of rice bacterial blight plate

[0040] The activated Xanthomonas oryzae was inoculated into LB liquid medium for shaking flask culture at 28°C and 200rpm....

Embodiment 3

[0055]Example 3: The fermented liquid of bacterial strain JS-1 was tested on the biocontrol effect of bacterial blight of rice on potted plants. In order to test the biocontrol effect of bacterial strain SJ-1, this experiment was carried out.

[0056] Experimental steps:

[0057] 1.1 Germination of rice seeds

[0058] The variety of rice seeds is Huanghuazhan, first sterilized with 5% (w / v) sodium hypochlorite for 1 hour, soaked at 37°C for 24 hours, then spread the soaked seeds on a glass petri dish (with thin density) and germinate in the dark For 48 hours, pay special attention not to leave water in the glass petri dish when germination, just keep the seeds moist all the time; before sowing, select seeds with more consistent germination.

[0059] 1.2 Seedling transplanting

[0060] Fill the flower pots (50×20×16cm) with mud pond soil, compact them slightly, then move 6 rice seedlings into each pot, press the roots slightly with two fingers after watering, and cultivate th...

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Abstract

The invention discloses staphylococcus haemolyticus applied to preventing and treating xanthomonas oryzae pv. oryzae and application thereof. The staphylococcus haemolyticus is named as staphylococcus haemolyticus JS-1, a preservation number is CCTCC (China Center for Type Culture Collection) NO: M 2017308, and the staphylococcus haemolyticus is preserved in China Center for Type Culture Collection in Wuhan University in Wuhan of China on June 6th 2017. The staphylococcus haemolyticus has an excellent antagonism effect on the xanthomonas oryzae pv. oryzae, further field experiments can be performed based on the xanthomonas oryzae pv. oryzae, and mature microbial organic fertilizer can be developed; thus, use of a lot of fertilizer and pesticides is reduced, and the staphylococcus haemolyticus has important significance on reducing agricultural non-point source pollution.

Description

technical field [0001] The invention belongs to the field of microbial screening, and in particular relates to a hemolytic staphylococcus strain used for preventing and treating bacterial blight of rice and its application. Background technique [0002] Rice bacterial blight is an important bacterial disease of rice, which mainly occurs in parts of Asia, North America and Africa. In Asia, rice in southern China and South Asian countries is relatively serious. The affected rice generally loses 20% to 30% of its output, and in severe cases it can reach 50%, or even no harvest. Rice bacterial blight (Xoo) is a Gram-negative bacterium with a short rod-shaped body, single flagella, polar or subpolar, without spores and capsules, and is surrounded by mucilage exopolysaccharides. The rice bacterial blight fungus infects the vascular bundles of rice leaves through water holes or wounds to cause rice bacterial blight. [0003] Rice bacterial blight is also known as white leaf blast...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00C12R1/44
CPCA01N63/00C12N1/205C12R2001/44
Inventor 邓音乐宋施豪孙秀云付书娜司阳神方芳邓海飞
Owner SOUTH CHINA AGRI UNIV
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