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Bacillus cereus H-B-1 for effectively preventing and treating crown gall of prunus serrulata and application thereof

A technology of Bacillus cereus and Agrobacterium tumefaciens is applied in the field of microorganisms to achieve the effects of simple culture conditions, good control effect and easy preservation

Inactive Publication Date: 2017-09-12
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In recent years, there are few reports on the research on Bacillus in the field of biological control of cherry root cancer

Method used

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  • Bacillus cereus H-B-1 for effectively preventing and treating crown gall of prunus serrulata and application thereof
  • Bacillus cereus H-B-1 for effectively preventing and treating crown gall of prunus serrulata and application thereof
  • Bacillus cereus H-B-1 for effectively preventing and treating crown gall of prunus serrulata and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Example 1: Bacillus cereus strain H-B-1 Their inhibitory effect on the growth of the Tumefaciens strain was determined by the agar diffusion method.

[0025] The purified cultured Agrobacterium tumefaciens and the bacteria isolated in the soil were made into 10 7 ~10 8 For cfu / mL bacterial suspension, mix the Agrobacterium tumefaciens A-4 bacterial suspension with the modified 523 medium according to the inoculum amount of 5%, and shake well. Add 25mL of modified 523 medium mixed with Agrobacterium tumefaciens into each dish, and after solidification, punch 2 to 3 holes on the medium with a puncher, add 130μL of soil bacterial suspension to each hole, and culture at 28°C 24h ~ 48h, measure the diameter of the inhibition zone. The results show that if figure 1 As shown, the left picture is the antibacterial map, and the right picture is the CK control. It can be seen that the Bacillus cereus strain H-B-1 has a good antibacterial effect, and the antibacterial diameter ...

Embodiment 2

[0026] Example 2: Determination of plate bacteriostatic ability of Bacillus cereus strain H-B-1 fermentation broth and Rhizobacillus strain.

[0027]The glycerin preservation solution of Bacillus cereus strain H-B-1 was inoculated into beef extract peptone liquid medium at an inoculum size of 2%, and shaken for 24 hours. Filter the bacterial fermentation liquid with a 0.22 μm bacterial filter to obtain the bacterial fermentation filtrate. Mix the bacterial fermentation filtrate of each strain of bacteria with the culture medium (improved 523 culture 25mL) according to the inoculum size of 20%, take a filter paper piece with a diameter of 0.6cm and dip it in the bacterial suspension of Agrobacterium tumefaciens, and place it after moderately air-drying On the plate mixed with the bacterial fermentation filtrate, 2 to 3 filter paper sheets per plate, and measure the diameter of the colony after culturing in the incubator for 48 hours. As a result, it was found that the strain H...

Embodiment 3

[0028] Embodiment 3: the disease resistance of Bacillus cereus bacterial strain H-B-1 to greenhouse indicator plant root cancer

[0029] Purchase tomato seedlings and grow them in mixed culture soil. Inoculate when the seedlings protrude about 15cm from the soil surface (select robust seedlings with similar growth), take out the bacteria to be inoculated (separated 6 strains of soil bacteria) half an hour before inoculation, return the temperature of the cultured bacteria to room temperature, and then start the inoculation. First, burn the dissecting needle on an alcohol lamp to sterilize it. After the dissecting needle is cooled to room temperature, pick the isolated and purified bacteria and pierce the stems of the seedlings. Use degreased cotton pads to dip in sterile water and bind them with a parafilm at the inoculation site. , Moisturize for 5-7 days; take out the bacteria to be inoculated half an hour before inoculating the sakura root disease bacteria, let the temperat...

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Abstract

The invention discloses a bacterial strain for effectively preventing and treating the crown gall of prunus serrulata. The bacterial strain is classified and named as bacillus cereus H-B-1, and is already collected in the China Center for Type Culture Collection (CCTCC); the collection number is CCTCC NO: M 2017214; the collection date is April 27, 2017; the collection address is Wuhan University in Wuhan, China. The bacillus cereus H-B-1 strain provided by the invention is biocontrol plant growth-promoting rhizobacteria, has better prevention and treatment effects on the crown gall of the prunus serrulata, and meanwhile has a growth-promoting effect on the growth of the prunus serrulata. The bacillus cereus H-B-1 strain is simple in culture condition, is easy to store, is easy in industrialized production and has favorable development and application prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a strain of Bacillus cereus H-B-1 which can effectively prevent and cure cherry blossom root cancer and its application. Background technique [0002] Cherry blossoms are world-renowned ornamental flowers and trees, belonging to the Rosaceae (Rosaceae) genus Cerasus, distributed in warm regions of the northern hemisphere, and are northern temperate plants. Cherry blossoms are shallow-rooted plants that like slightly acidic, loose, fertile, well-drained soil and a leeward, sunny and humid environment. my country has very rich cherry blossom resources, far more than Japan and neighboring countries. The distribution range is very wide, from the northeast to the southwest, and there are many species with high ornamental value. In recent years, cherry blossoms have attracted extensive attention from nursery stock breeders because of their beautiful plant type, gorg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P1/00A01P21/00C12R1/085
CPCA01N63/00A01N63/10C12N1/205C12R2001/085
Inventor 叶建仁王晓东万其露吴小芹
Owner NANJING FORESTRY UNIV
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