Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nattokinase-producing bacillus velezensis strain and application thereof

A technology of bean kinase Bacillus velaisi and bacillus, applied in the field of microbiology, can solve the problem of undiscovered Bacillus velaisi nattokinase, etc., achieve good taste, high vitality, and improve the effect of food taste

Active Publication Date: 2017-09-05
GUIZHOU UNIV
View PDF4 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Until now, in domestic and foreign studies, it has not been found that Bacillus Veles is used in the production of nattokinase

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nattokinase-producing bacillus velezensis strain and application thereof
  • Nattokinase-producing bacillus velezensis strain and application thereof
  • Nattokinase-producing bacillus velezensis strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A kind of Veles bacillus SN-14, its screening process is as follows:

[0034] (1) Primary screening

[0035] Take 2g each of 9 kinds of samples from Zunyi Douchi, Guiyang Douchi, Dafang Stinky Tofu, Tongren Douchi, Bijie Stinky Douche, Anshun Douchi and Qingyan Douchi in Guizhou Province, and place them in a 100mL sterilized triangular flask with a stopper. Dilute 10-fold with sterile normal saline, shake for 30 seconds, and incubate for 24 hours at 37°C and 180 r / min. Heat the bacterial suspension in a water bath at 80-85°C for 10 minutes, then make gradient dilutions of the cooled bacterial suspensions, and spread 0.1 mL each on a casein plate, incubate upside down at 37°C for 24 hours, and pick out the hydrolyzed circle Colonies with a larger colony diameter ratio (C / H value) were subjected to Gram staining and microscopic examination, and the primary microscopic examination was carried out according to the description of the Bacillus genus in the common bacterial s...

Embodiment 2

[0063] Determination of fibrinolytic activity (nattokinase activity): adopt the agarose-fibrinogen plate method, and the preparation steps of the agarose plate are as follows:

[0064] First, dissolve 1% agarose in 0.05mol / L Tris-HCl (pH7.8) buffer solution, heat until completely dissolved, take out 10mL and place it in a large test tube, wait for it to cool to about 50°C, and pour it quickly Add 10 mL of 1.5 mg / L bovine fibrinogen solution, oscillate continuously to make it completely mixed evenly, pour it into a 9cm diameter petri dish, then quickly add 300 μL of 20IU / mL thrombin solution, and shake the dish continuously to mix the three evenly , to prevent the generation of air bubbles, after standing for 1 h, use a sterile plastic pipette with a diameter of 2 mm to punch holes on the plate.

[0065] The making of urokinase standard curve: this urokinase standard substance (1240IU / bottle) is prepared as 508, 635, 794, 992 and 1240IU / mL (carry out according to the gradient d...

Embodiment 3

[0069] A kind of application of Bacillus veleus SN-14 in preparing natto, its steps are as follows:

[0070] Transfer the Bacillus veleisi SN-14 strain to LB slant medium for activation at 37°C for 24 hours, use an inoculation loop to select 2-3 rings of bacterial lawn and inoculate it into the liquid seed medium (filling volume 50mL / 250mL), Cultivate at 37°C and 180r / min for 16h to the logarithmic growth phase, then inoculate the sterilized soybean culture at an inoculum size of 4% (v / w), cultivate at 37°C for 36h, and stir once every 12h. The color of the fermented natto is khaki, the bean grains are soft, and the soybeans have a long-drawn phenomenon when you stir them, with a faint aroma. The activity of nattokinase kinase is 6583.2IU / g (wet soybeans).

[0071] The liquid seed medium: 10g / L glucose, 5g / L yeast extract, 10g / L beef extract, 5g / L NaCl, pH 7.4-7.6.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a nattokinase-producing bacillus velezensis strain which is characterized in that the strain is bacillus velezensis SN-14. Compared with the prior art, the strain disclosed by the invention has the advantages that (1) the bacillus velezensis SN-14 in the invention comes from local fermented soya beans in Guizhou, and the safety of the strain is high; (2) natto products obtained from the bacillus velezensis SN-14 in the invention have excellent taste; and (3) the bacillus velezensis SN-14 disclosed by the invention has high nattokinase-producing activity and is a primary condition for researching and developing nattokinase products. Therefore, the bacillus subtilis has excellent application prospects for improving the food taste, improving the fermentation activity and enriching effects of natto health care products.

Description

technical field [0001] The invention belongs to the technical field of microbes, and specifically relates to a new nattokinase-producing strain, and also relates to the application of a nattokinase-producing strain in preparing natto. Kinase ability can be used in the production of natto. Background technique [0002] Thrombotic diseases seriously affect human health. According to statistics, at least 12 million patients die of thrombotic diseases every year in the world, and about 2.6 million in China. Therefore, the development of thrombolytic and antithrombotic drugs has always been a hot spot related to human health. Currently clinically commonly used thrombolytic and antithrombotic drugs include streptokinase (Streptokinase, SK), urokinase (Urokinase, UK) and tissue-type plasminogen activator (type plasminogen activator, t-PA). Pain, side effects, expensive, short half-life and other disadvantages, in contrast, Nattokinase (Nattokinase, NK) has been proven to have a h...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L11/00C12R1/07A23L11/50
CPCC12N1/20C12R2001/07C12N1/205
Inventor 何腊平高泽鑫李翠芹
Owner GUIZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com