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Pseudomonas strain for preventing and treating kiwifruit canker and application of pseudomonas strain

A technology for kiwifruit canker and Pseudomonas, applied in the field of microorganisms, can solve the problems affecting the field control effect of canker, the problem of drug resistance of canker bacteria, etc., and achieves the effects of stable nature, strong inhibitory effect and remarkable effect.

Inactive Publication Date: 2017-08-22
四川省自然资源科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extensive use of copper preparations has caused the resistance of canker bacteria, and the food safety problems caused by the extensive use of antibiotics have seriously affected the field control effect of canker

Method used

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  • Pseudomonas strain for preventing and treating kiwifruit canker and application of pseudomonas strain
  • Pseudomonas strain for preventing and treating kiwifruit canker and application of pseudomonas strain
  • Pseudomonas strain for preventing and treating kiwifruit canker and application of pseudomonas strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Isolation and screening of Pseudomonas CCTCC M2016556

[0026] The Pseudomonas CCTCC M2016556 bacteria source of the present invention comes from wild kiwi branches in Jinggangshan, Jiangxi, the altitude of the sampling point is 1414m, and the longitude and latitude are N26°36'30, E114°07'33;

[0027] The separation method is: 1. Rinse the collected kiwi branches with clear water, disinfect the surface with 75% alcohol, air-dry at room temperature, cut an appropriate amount of sample with a sterilized blade, put it into a grinding bag, add an appropriate amount of normal saline, grind, After soaking for 3 minutes, take 100 μL of the supernatant of the plant grinding liquid and add it to the newly prepared KB medium, and use an inoculation loop to coat and streak.

[0028] The purification method is as follows: After cultivating the sample petri dish at room temperature for a certain period of time, wait for the colonies to grow in the dish, pick the colonies w...

Embodiment 2

[0029] Example 2 Identification of Pseudomonas CCTCC M2016556

[0030] 1. Observation of culture status

[0031] Activate the preserved strains on KB solid medium, culture them at room temperature, observe the growth of the colonies, and record the characteristics of the colonies, including color, growth speed, shape, texture, etc.

[0032] It is finally found that the Pseudomonas CCTCC M2016556 strain of the present invention is milky white, colloidal, with a smooth and moist surface, a raised center, neat edges, and normal viscosity; its strain morphology is shown in figure 1 Shown; its scanning electron microscope picture is shown in figure 2 shown.

[0033] 2. Molecular biological identification

[0034] DNA extraction: use bacterial PCR universal primer 27F (primer sequence is:

[0035] Eubac27F[106]5'-AGAGTTTGATCMTGGCTCAG-3') and 1492R (primer sequences are:

[0036] Eubac1492R[106]5'-TACGGYTACCTTGTTACGACT-3') amplified the 16S rDNA of the strain, and the length of...

Embodiment 3

[0037] Example 3 Screening of antagonistic bacteria for kiwifruit canker

[0038] 3.1 Test material

[0039] Actinidia endophytes isolated from kiwifruit samples collected from Dongshanfeng in Hunan Province, Jinggangshan in Jiangxi Province and Sichuan Province.

[0040] The pathogenic bacteria of kiwifruit canker for testing: Pseudomonas syringae pv.Actinidiae, the pathogen of kiwifruit bacterial canker isolated and identified by Sichuan Natural Resources Science Research, is shown in Table 1:

[0041] Table 1 Pathogens tested

[0042] Numbering Canker disease (Pseudomonas syringae pv.actinidiae.Psa) strains tested PC psa2012DJY P1 psa2013DJY P6 psa2013PJ 155 psa2014EB 185 psa2014YJ 206 psa2014QL

[0043] Screening of endophytic antagonistic bacteria against kiwifruit canker:

[0044] In this experiment, the Oxford cup method was used to screen the endogenous antagonistic bacteria of kiwifruit canker. The specific steps a...

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Abstract

The invention relates to the technical field of microorganisms and particularly relates to a pseudomonas strain for preventing and treating kiwifruit canker and application of the pseudomonas strain. The Pseudomonas (pseudomonas sp.) strain provided by the invention has a preservation number of CCTCC NO: M2016556. The pseudomonas is obtained by separating and purifying collected kiwifruit twigs, a separation and screening method is simple, and the strain has very strong inhibition effect to pathogenic bacteria of kiwifruit bacterial canker and is capable of obviously inhibiting the growth of the pathogenic bacteria of the kiwifruit bacterial canker. The strain is separated from healthy plant tissues and is inoculated to the kiwifruit twigs, leaves are not abnormal, and a generated active metabolite has no obvious toxic effect to the plant tissues; the strain has stable property and remarkable effects on treating and preventing the kiwifruit canker and is easily wetted through spraying and beneficial to the environmental protection, and an environment-friendly, simple and effectively way is provided for the control and treatment of the kiwifruit canker.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a pseudomonas strain for preventing and treating kiwifruit canker and application thereof. Background technique [0002] Kiwifruit canker is a devastating bacterial disease. The disease mainly harms the trunk, branches, shoots and leaves of kiwifruit, and can easily cause plant death. The disease was discovered for the first time in Japan in 1980, and it was discovered successively in the United States after 1983. In 1987, the pathogen was identified as Pseudomonas syringaepv.actinidiae, the pathogenic variant of Pseudomonas syringa kiwifruit. Before 1984, there was no kiwifruit bacterial canker in my country. It was first discovered in Dongshanfeng Farm, Hunan Province in 1985, and spread rapidly to most kiwifruit cultivation areas in my country in just 10 years. The disease is ferocious and often breaks out on a large scale, resulting in large-scale destruction of gar...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P1/00C12R1/38
CPCA01N63/00A01N63/10C12N1/20C12N1/205C12R2001/38
Inventor 庄启国李明章何利钦王永志王丽华张茜
Owner 四川省自然资源科学研究院
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