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High induced activity repairing material and preparation method and application thereof

A technology for repairing materials and inducing activity, which is applied in medical science, prostheses, tissue regeneration, etc. It can solve the problems of short half-life of growth factors and achieve the effect of long action period and controllable degradation process

Active Publication Date: 2017-08-22
BEIJING BIOSIS HEALING BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the half-life of growth factors in the body is short, so the release behavior in the body needs to be further resolved

Method used

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  • High induced activity repairing material and preparation method and application thereof
  • High induced activity repairing material and preparation method and application thereof
  • High induced activity repairing material and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The preparation method of the animal-derived implantable biological patch in this embodiment includes the following steps:

[0051] (1) Organization pre-processing:

[0052] Take the small intestinal submucosa tissue material and divide it into the specified size of 10cm in width and 15cm in length, remove the lymphoid tissue, wash it with tap water for 3 times, and then rinse it with purified water until there is no stain on the surface, and place the washed small intestinal submucosa tissue material on the filter Wait for the water filter device, let it stand for more than 5 minutes, and drain the water.

[0053] (2) Virus inactivation:

[0054] Use peracetic acid-ethanol solution to soak small intestinal submucosa tissue material for virus inactivation, and this process can be carried out in a stainless steel bucket. The volume percent concentration of peracetic acid in the peracetic acid-ethanol solution is 2%, the volume percent concentration of ethanol is 20%, a...

Embodiment 2

[0072] Example 2: Performance testing

[0073] 1) The heterogeneous immunogen removal matrix material with high inductive activity is a three-layer structure, the upper and lower surface layers are complete surface layers, and the middle layer is a layer containing growth factors, forming a sandwich structure. The surface layer is relatively dense, which can control the degradation behavior of the material and growth factor release speed, and the growth factor layer is a porous structure layer, which is beneficial to preserve the growth factor. The porosity of the surface layer is 67.4±5.2%, while that of the growth factor layer is 90.5±7.0%.

[0074] 2) The test of mechanical properties shows that the tensile breaking strength of the sample reaches 105N / cm, and the suture holding force is greater than 21N.

[0075] 3) According to the fourth part of the biological preparation residual DNA detection method "Chinese Pharmacopoeia" 2015 edition, the residual DNA of the sample p...

Embodiment 3

[0081] Embodiment 3: the detection of active factor release curve

[0082]Removal of heterogeneous immunogens with highly inducible activity carrier proteins The matrix material was prepared according to Example 1. The samples were placed in a 12-well plate, and 2 ml of PBS (pH 7.4) was added to each well. 5% CO at 37°C with saturated humidity 2 Atmosphere CO 2 Incubate in the incubator for 1, 3, 5, 7, 14, 21, 28 days (days), then transfer the PBS to a centrifuge tube, and detect the content of platelet-derived growth factor M1 in the PBS solution, and add the initial addition amount as the denominator M0 , Calculate growth factor release rate = M1 / M0. as attached image 3 As shown, the release of growth factors was stable and controllable. The growth factor release rate was 19.92±1.96% on the first day, 46.32±3.36% on the third day, 64.16±2.50% on the seventh day, and the total release rate on the 28th day The rate reaches 86.56±3.42%. Therefore, the growth factor can la...

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Abstract

The invention provides a high induced activity repairing material and a preparation method and application thereof. The repairing material is a natural polymer material having a 3D network-like porous structure. The repairing material has a three-layer structure composed of a middle layer and upper and lower surfaces. The middle layer contains collagen, a polysaccharide substance, an active factor and a growth factor. The upper and lower surfaces comprise collagen, a polysaccharide substance and an active factor. The repairing material can be degraded in vivo, can contain one or more growth factors such as a follicle inhibitory protein, a nerve growth factor, a platelet-derived growth factor, an epithelial growth factor and a matrix gamma-carboxyl glutamate protein, and can be used in myocardial, nerve, smooth muscle and epithelial tissue growth and regulation or prevent the post-implantation calcification of a matrix material without an immunogen.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, in particular to a repair material with high inductive activity, a preparation method and application. The material is used to compound growth factors into an immunogen-removing matrix to enhance the inductive repair effect of the material on specific tissues. Background technique [0002] Immunogen-removed small intestinal submucosa (SIS) material is a biomedical material widely used in clinical practice for the repair and reinforcement of soft tissue defects such as abdominal wall, urinary tract, dura mater, and blood vessel repair. It is a cell-free, non-immunogenic , Biodegradable materials with good fiber elasticity. [0003] Invention patent (103272278A) once disclosed a preparation method of animal-derived implantable medical biomaterials, which introduced pre-treatment separation, initial washing, virus inactivation, immunogen removal, and sodium chloride treatment of animal ...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/36A61L27/24A61L27/20A61L27/54A61L27/56A61L27/58
CPCA61L27/20A61L27/24A61L27/3629A61L27/3687A61L27/3691A61L27/54A61L27/56A61L27/58A61L2300/252A61L2300/412A61L2300/414A61L2300/602A61L2430/30A61L2430/32A61L2430/40C08L5/08
Inventor 赵博夏磊磊王洪权赵延瑞李学军张晋辉
Owner BEIJING BIOSIS HEALING BIOLOGICAL TECH
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