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Method for producing fuel ethanol by multi-strains co-fermentation of straws

A technology of fuel ethanol and fermentation bacteria, which is applied in the fields of fermentation engineering and biomass energy, and can solve problems such as strain degradation

Inactive Publication Date: 2017-07-07
STATE GRID CORP OF CHINA +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of ethanol fermentation using genetically recombinant bacteria, there are also problems such as strain degradation

Method used

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  • Method for producing fuel ethanol by multi-strains co-fermentation of straws
  • Method for producing fuel ethanol by multi-strains co-fermentation of straws

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Take commercially available glucose and xylose samples respectively, add water to mix glucose and xylose (the mass ratio of glucose and xylose is 4:1), wherein, the concentration of glucose is: 100g / L, and the concentration of xylose is: 25g / L L, add the yeast powder of 3g / L and other trace elements (the addition amount of other trace elements is: 1g / L potassium dihydrogen phosphate, 0.5g / L magnesium sulfate), obtain fermentation medium, sterilize for subsequent use.

[0070] Choose Zymomonas mobilis, inoculate to seed culture medium after activation (the formula of seed culture medium is: glucose 50g / L, KH 2 PO 4 2g / L, yeast powder 10g / L, pH7.0), after cultivating for 12h, the seed liquid of the bacterium was obtained.

[0071] Select Pachysolomyces tannophilus, inoculate it into the seed medium after activation (the formula of the seed medium is: glucose 20g / L, tryptone 3.0g / L, yeast extract 5.0g / L), after culturing for 48h, the obtained Bacteria seed fluid.

[00...

Embodiment 2

[0074] Crush corn stalks to a particle size of 20 mm, prepare the stalks to a concentration of 60 g / L, add commercially available cellulase such as Spezyme CP produced by Genenco and xylanase preparations such as Accellerase XC produced by Genenco, and the addition amounts are respectively 15U / g of straw was hydrolyzed for 48h at pH 7.0 and 50°C to prepare a mixture of glucose and xylose, and 2g / L of yeast powder and other trace elements were added (the amount of other trace elements: 3g / L potassium dihydrogen phosphate, 1g / L magnesium sulfate), obtain fermentation medium, sterilize for subsequent use.

[0075] Select Saccharomyces cerevisiae, inoculate to seed medium after activation (the formula of seed medium is: glucose 40g / L, KH 2 PO 4 3g / L, yeast powder 5g / L, pH7.0), after culturing for 18h, the seed liquid of the bacterium was obtained.

[0076] Select Candida shohata, inoculate into seed medium after activation (the formula of seed medium is: glucose 30g / L, trypton...

Embodiment 3

[0079] Crush the wheat straw to a particle size of 30mm, add 0.2mol / L H 2 SO 4 The solution is treated for 24 hours, the pretreated straw is washed with water until the pH is 6.0-7.0, and dried. Prepare the pretreated straw to a concentration of 70g / L, add commercially available cellulase such as Spezyme CP produced by Genencor or xylanase preparation such as Accellerase XC produced by Genencor, the addition amount is 10U / g straw, in pH 6.0, hydrolysis at 50°C for 48 hours to prepare a mixture of glucose and xylose, add 3g / L yeast powder and other trace elements (addition of other trace elements: 1g / L potassium dihydrogen phosphate, 0.5g / L L magnesium sulfate), to obtain the fermentation medium, sterilized for subsequent use.

[0080] Choose Kluyveromyces, inoculate to seed culture medium after activation (the prescription of seed culture medium is: glucose 50g / L, KH 2 PO 4 5g / L, yeast powder 10g / L, pH 7.0), after culturing for 24 hours, the seed liquid of the bacterium w...

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Abstract

A method for producing fuel ethanol by multi-strains co-fermentation of straws comprises the following steps: (1) taking mixed liquor of glucose and xylose or hydrolysate of biomass (such as maize straws, wheat straws or rice straws) as a carbon source, and adding yeast powder and microelements to obtain a fermention culture medium; and (2) simultaneously inoculating a seed solution of glucose zymophyte and a seed solution of xylose zymophyte into the fermention culture medium according to the ratio of 1: 1-1: 3(v / v), and fermenting for 36-60 hours at the temperature of 25-38 DEG C. By the method, glucose can be efficiently transformed into ethanol, meanwhile, the xylose is transformed into ethanol to a maximum degree, the yield of glycerinum which is a by-product in fermentation broth is remarkably reduced, steps of extraction and purification are improved remarkably, and the production cost of cellulosic ethanol is reduced obviously.

Description

technical field [0001] The invention belongs to the fields of fermentation engineering and biomass energy, and relates to a method for producing fuel ethanol by using multi-strain mixed fermentation straw, which can convert glucose and xylose degraded from straw or pretreated straw into ethanol, realizing Thorough utilization of glucose and xylose in straw. Background technique [0002] The production of fuel ethanol from lignocellulose is a hot topic in the current energy field. Lignocellulosic substances are mainly composed of cellulose, hemicellulose and lignin. The cellulose in the lignocellulosic raw material accounts for about 35-45% of the dry weight, and the hemicellulose accounts for about 20-40%. The hydrolyzate of cellulose is glucose; while the hydrolyzate of hemicellulose is more complex, mainly monosaccharides such as xylose and glucose. Among all these hydrolysates, the content of glucose and xylose accounted for the vast majority. Therefore, the efficient...

Claims

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Application Information

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IPC IPC(8): C12P7/10C12P19/14C12R1/645C12R1/77C12R1/84C12R1/72C12R1/07C12R1/19C12R1/01
CPCC12P7/10C12P19/14Y02E50/10
Inventor 管国强赵锦崔凤杰卜令习黄达明赵正凯
Owner STATE GRID CORP OF CHINA
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