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High-quantum-yield electrochemical luminous gold nanometer cluster probe prepared by electrochemical reduction method

A gold nanocluster, electrochemical technology, applied in the nano field, can solve the problems of weak electrochemiluminescence intensity, less research on electrochemiluminescence sensing, etc., and achieves a green preparation method, good application prospects, and good reproducibility. Effect

Inactive Publication Date: 2017-06-20
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the fluorescence properties and applications of gold nanoclusters have been extensively studied, due to the weak ECL intensity and unknown mechanism, the research on ECL sensing based on gold nanoclusters is still very little.

Method used

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  • High-quantum-yield electrochemical luminous gold nanometer cluster probe prepared by electrochemical reduction method
  • High-quantum-yield electrochemical luminous gold nanometer cluster probe prepared by electrochemical reduction method
  • High-quantum-yield electrochemical luminous gold nanometer cluster probe prepared by electrochemical reduction method

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Experimental program
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Effect test

Embodiment 1

[0038]Add 0.6 mL of 0.5 mol / L sodium hydroxide and 0.4 mL of 20 mg / mL chloroauric acid solution to 4 mL of 0.08 mol / L N-acetyl-L-cysteine ​​solution, mix After uniformity, they were placed in a 37°C constant temperature water tank and incubated for 3 hours. After the reaction, the reaction solution was purified by dialysis with a dialysis bag with a molecular cut-off of 3500 to obtain an aqueous solution of N-acetyl-L-cysteine-gold nanoclusters, which was freeze-dried to obtain N-acetyl-L-cysteine Acid-gold nanocluster powder. Take the above-mentioned N-acetyl-L-cysteine-gold nanocluster aqueous solution for fluorescence spectrum analysis, and the maximum excitation wavelength and emission wavelength can be obtained as 355 nm and 650 nm respectively (see figure 1 ).

Embodiment 2

[0040] Glassy carbon electrodes with a diameter of 3 mm were coated with 1.0 μm, 0.3 μm and 0.05 μm Al 2 o 3 The powder is polished to a smooth mirror surface, and then put in HNO in sequence 3 Solution (volume ratio of concentrated nitric acid to water is 1:1), absolute ethanol, ultrasonic cleaning in deionized water for 3 minutes, N 2 blow dry. Take 5 μL of N-acetyl-L-cysteine-gold nanocluster solution and drop it on the surface of the treated glassy carbon electrode, and dry it at room temperature to obtain N-acetyl-L-cysteine-gold nanocluster modification Glassy carbon electrode. Insert the above electrodes into 0.1 mol / L pH 7.4 phosphate buffer solution containing 0.1 mol / L potassium persulfate and 0.1 mol / L KCl. The step pulse method was adopted, the initial potential was 0 V, the pulse time was 10 s, the end potential was -2 V, and the pulse time was 1 s. The high voltage of the photomultiplier tube was set to 700 V, and the electrochemiluminescence signal generate...

Embodiment 3

[0042] A three-electrode system was used for electrochemical reduction. The N-acetyl-L-cysteine-gold nanocluster modified glassy carbon electrode was used as the working electrode, the platinum wire electrode was used as the counter electrode, and Ag / AgCl was used as the reference electrode. The electrode was inserted into 0.1 mol / L phosphate buffer solution with pH 7.4, and subjected to potentiostatic reduction with a reduction potential of -2.0 V and a reduction time of 5 minutes to obtain a reduced gold nanocluster probe-modified glassy carbon electrode. The prepared reduced gold nanocluster probe-modified glassy carbon electrode was inserted into 0.1 mol / L pH 7.4 phosphate buffer solution containing 0.1 mol / L potassium persulfate and 0.1 mol / L KCl. The step pulse method was adopted, the initial potential was 0 V, the pulse time was 10 s, the end potential was -2 V, and the pulse time was 1 s. The high voltage of the photomultiplier tube was set to 700 V to detect the elect...

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Abstract

The invention discloses a high-quantum-yield electrochemical luminous gold nanometer cluster probe prepared by an electrochemical reduction method. The electrochemiluminescence gold nanometer cluster probe uses a functional gold nanometer cluster material as a precursor; then, an electrochemical method is used for reduction treatment. The electrochemiluminescence gold nanometer cluster probe is modified on an electrode; peroxydisulfate ions are used as a co-reaction agent; good electrochemical luminous performance is realized. The cluster probe has the characteristics that the preparation method is simple; the reaction conditions are mild; the reproducibility is good; the probe electrochemiluminescence signal intensity is high; the quantum yield is high, and the like. Along with the rapid development of an electrochemiluminescence sensor technology, the condition that the probe has great significance in the fields of chemistry, biology, medicine, environment and the like can be expected.

Description

technical field [0001] The invention relates to a high quantum yield electrochemiluminescence gold nano-cluster probe material and an electrochemical reduction preparation method thereof, belonging to the field of nanotechnology. Background technique [0002] In recent years, semiconductor nanocrystals or quantum dots, as a new type of electrochemiluminescent nanoluminophores, have received extensive attention due to their advantages such as good stability, strong resistance to photobleaching, and wide excitation spectrum. Electrochemiluminescence of semiconductor nanocrystals or quantum dots combines the advantages of semiconductors, electrochemistry and chemiluminescence to become a new field in electrochemiluminescence analysis and is developing rapidly. However, defects such as strong toxicity and non-molecular power-law fluorescence intermittent phenomenon bring difficulties to the research of tracking and imaging, especially in vivo applications. Therefore, it is immin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/36G01N27/30G01N21/76C09K11/58C25C1/20
CPCC09K11/58C25C1/20G01N21/76G01N27/308G01N27/327G01N27/36
Inventor 彭花萍简美丽陈伟邓豪华黄种南
Owner FUJIAN MEDICAL UNIV
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