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Preparation method for extraction of catechin from tea

A catechin and tea technology, which is applied in the field of preparation of catechin extraction, can solve the problems of difficult industrial control, large solvent consumption, low extraction rate, etc., and achieve the effects of easy industrial control, simple operation and high sample purity

Inactive Publication Date: 2017-06-16
XIAN SHIQING MEDICINE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the problems of complex operation, large solvent consumption, low extraction rate and difficult industrial control in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Dry the tea leaves at 80°C, pass through a 80-mesh sieve to form tea samples; weigh 100g of tea samples into a polytetrafluoroethylene digestion tank, add an ethanol solution with a mass concentration of 60% at a material-to-liquid ratio of 1:80, Tighten the mouth of the tank, then place it in a microwave digestion apparatus, set the power to 720W, time to 5min, temperature to 80°C, carry out microwave leaching, then filter and wash, and distill under reduced pressure to recover ethanol to obtain the extract; use the extract to Repeated extraction with equal amount of chloroform, standing to separate layers, combining the aqueous solution, then repeatedly extracting the aqueous solution with the same amount of ethyl acetate, removing the water phase, distilling the ester phase under reduced pressure to recover ethyl acetate, then concentrating in a vacuum and freeze-drying to obtain Catechin crude extract. The crude catechin extract was pre-separated by chromatography, ...

Embodiment 2

[0021] Dry the tea leaves at 90°C, pass through a 80-mesh sieve to form tea samples; weigh 100 g of tea samples into a polytetrafluoroethylene digestion tank, add an ethanol solution with a mass concentration of 70% at a material-to-liquid ratio of 1:80, Tighten the mouth of the tank, then place it in a microwave digestion apparatus, set the power at 800W, time at 6min, and temperature at 90°C, carry out microwave leaching, then filter and wash, and recover ethanol by vacuum distillation to obtain the extract; use the extract to Repeated extraction with equal amount of chloroform, standing to separate layers, combining the aqueous solution, then repeatedly extracting the aqueous solution with the same amount of ethyl acetate, removing the water phase, distilling the ester phase under reduced pressure to recover ethyl acetate, then concentrating in a vacuum and freeze-drying to obtain Catechin crude extract. The crude catechin extract was pre-separated by chromatography, and th...

Embodiment 3

[0023] Dry the tea leaves at 100°C, pass through a 80-mesh sieve to form tea samples; weigh 100g of tea samples into a polytetrafluoroethylene digestion tank, add an ethanol solution with a mass concentration of 80% according to a material-to-liquid ratio of 1:80, Tighten the mouth of the tank, then place it in a microwave digestion apparatus, set the power to 900W, time to 7min, temperature to 100°C, carry out microwave leaching, then filter and wash, and distill under reduced pressure to recover ethanol to obtain the extract; use the extract to Repeated extraction with equal amount of chloroform, standing to separate layers, combining the aqueous solution, then repeatedly extracting the aqueous solution with the same amount of ethyl acetate, removing the water phase, distilling the ester phase under reduced pressure to recover ethyl acetate, then concentrating in a vacuum and freeze-drying to obtain Catechin crude extract. The crude catechin extract was pre-separated by chro...

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Abstract

The invention relates to a preparation method for extraction of catechin from tea, belonging to the field of separation and extraction technology. The method comprises the following steps: drying tea and grinding the dried tea to obtain a tea sample; weighing the tea sample, adding an ethanol solution, placing the obtained mixture in a microwave digestion instrument for extraction and carrying out filtering and washing so as to obtain extract; adding chloroform into the extract for extraction so as to obtain an aqueous solution, then extracting the aqueous solution with ethyl acetate, removing a water phase, subjecting an ester phase to pressure-reduced distillation so as to recover ethyl acetate and carrying out vacuum concentration and freeze drying so as to obtain crude catechin extract; and subjecting the crude catechin extract to chromatographic pre-separation at first and then to preparative HPLC purifying and refining so as to obtain high-purity catechin. The method provided by the invention is simple in operation process, low in the usage amount of solvents, high in extraction rate and applicable to both small-scale preparation in laboratories and large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of separation and extraction, and relates to a preparation method for extracting catechins from tea leaves. Background technique [0002] Tea polyphenols is the general term for polyphenols in tea, mainly composed of four types of substances: catechins, flavonoids, anthocyanins and phenolic acids, the most important of which are flavanols with catechins as the main body. , its content accounts for more than 70% of the total polyphenols. Mao Qingli et al. summarized the health care and pharmacological functions of catechins as "three anti-cancer, anti-aging, anti-oxidation)" and "three lowering (lowering blood sugar, Lowering blood fat, lowering blood pressure)" and "three eliminations (disinfection, anti-inflammation, deodorization)" ("New progress in research on health care and pharmacological functions of tea catechins", Mao Qingli et al., "Food Science", Vol. 28, No. 8, Pages 584-589, August 2007), catec...

Claims

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Application Information

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IPC IPC(8): C07D311/62
Inventor 张明科
Owner XIAN SHIQING MEDICINE TECH CO LTD
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