Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for quickly analyzing free amino acid content in vegetable fresh sample

A free amino acid, rapid analysis technology, applied in the field of rapid analysis of free amino acid content in vegetables, can solve the problems of difficult promotion of basic application, slow speed, insufficient accuracy, etc., and achieve the effect of accurate and reliable measurement data

Active Publication Date: 2017-06-13
SUZHOU ACADEMY OF AGRI SCI
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a rapid analysis method for the content of free amino acids in fresh vegetables, and its purpose is to solve the problems of slow speed, low efficiency, insufficient accuracy and difficult basic application and popularization existing in the existing methods for amino acid analysis in vegetables

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for quickly analyzing free amino acid content in vegetable fresh sample
  • Method for quickly analyzing free amino acid content in vegetable fresh sample
  • Method for quickly analyzing free amino acid content in vegetable fresh sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0054] Example: A rapid analysis method for free amino acid content in fresh cabbage samples

[0055] Described rapid analysis method comprises following two parts:

[0056] The first part is to prepare the solution, and then use high performance liquid chromatography-fluorescence detection method to establish the standard curve of the measured free amino acid with known gradient concentration; the establishment of the standard curve consists of the following steps:

[0057] Step (1), prepare amino acid standards, respectively aspartic acid, serine, glutamic acid, histidine, glycine, arginine, threonine, alanine, proline, cystine, tyrosine amino acid, valine, methionine, lysine, isoleucine, leucine, phenylalanine;

[0058] Prepare instruments and equipment: 2695 high performance liquid chromatography with 2475 fluorescence detector (Waters Company); TG16-WS desktop high-speed centrifuge (Hunan Xiangyi Experimental Instrument Company); K600 pulverizer (German Braun Company); L...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
correlation coefficientaaaaaaaaaa
recovery rateaaaaaaaaaa
Login to View More

Abstract

The invention relates to a method for quickly analyzing free amino acid content in a vegetable fresh sample. The method is characterized by comprising the following steps: crushing and uniformly mixing the vegetable fresh sample; taking ultra-pure water as an extracting solvent; digesting for 20min under water bath at 90 DEG C; centrifugally filtering, and then taking 6-aminoquinolyl-N-hydroxysuccinimdyl carbamate as a derivation agent for pre-column derivatization; using an XBridge C18 chromatographic column for gradient eluting; and using a high performance liquid chromatography-fluorescence detector for separating and detecting. The method has the advantages of quick and simple sample preparation, low cost, quick determination, high precision, accuracy and stability and excellent linear relation; the whole analysis process is quick, sensitive and excellent in reproducibility; the method is suitable for quick analysis for the free amino acid in the vegetable fresh sample and is easy to be popularized and applied; and an ideal analysis method is supplied for identifying the quality of vegetables and developing and utilizing the vegetables.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a rapid analysis method for the content of free amino acids in vegetables. The rapid analysis method can simultaneously separate and analyze 17 kinds of free amino acids in fresh vegetable samples. Background technique [0002] Vegetables play an important role in people's daily dietary structure. They can provide the human body with rich nutrients such as vitamins, carotene, mineral elements and dietary fiber, and are deeply loved by consumers. Amino acids are important nutrients in vegetables. The content and composition of various amino acids directly affect their nutritional value and are closely related to human taste. Moreover, for vegetables, different kinds of amino acids are essential components in their growth and development, and play an important role in nitrogen assimilation, protein synthesis, and resistance to pests and diseases. Therefore, it is of great signi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/74G01N30/86G01N30/88
CPCG01N30/06G01N30/74G01N30/8634G01N30/88
Inventor 刘腾飞杨代凤李军范君孙灵湘钱辉
Owner SUZHOU ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com