A method for simultaneous determination of multi-component content in Shuxuening injection

An injection, multi-component technology, applied in the direction of measuring devices, material separation, analysis of materials, etc., can solve the problem of not being able to detect flavonoids and phenolic acids at the same time, and achieve low cost, good durability, and easy implementation Effect

Active Publication Date: 2019-05-24
HEBEI SHINEWAY PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above two methods have fewer detectable components and cannot detect flavonoids and phenolic acids at the same time, so it is necessary to conduct more in-depth research and develop better detection techniques

Method used

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  • A method for simultaneous determination of multi-component content in Shuxuening injection
  • A method for simultaneous determination of multi-component content in Shuxuening injection
  • A method for simultaneous determination of multi-component content in Shuxuening injection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0037] 1. Chromatographic conditions

[0038] Chromatographic column: ACQUITY UPLC BEH C18 (1.7μm, 2.1×150mm);

[0039] Mobile phase B: methanol / acetonitrile (1:1);

[0040] Mobile phase A: ultrapure water (containing 0.1% formic acid);

[0041] Flow rate: 0.3mL / min;

[0042] Column temperature: 35°C;

[0043] Injection volume: 1μL;

[0044] Diode array detector: use 280nm ultraviolet wavelength to detect phenolic acid components, and simultaneously use 360nm ultraviolet wavelength to detect flavonoid glycoside components;

[0045] Analysis time: 35min;

[0046] Gradient elution method:

[0047] time (min)

A(％)

B(％)

0

88

12

8

85

15

10

77

23

15

77

23

16

76

24

25

70

30

26

65

35

34

65

35

35

88

12

[0048] 2. Solution preparation

[0049] (1) Preparation of reference substance stock solution

[0050] Precisely weigh protocatechuic acid, cryptochl...

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Abstract

The invention relates to the field of medicine detection, in particular to a method for simultaneously determining the contents of multiple components in a Shuxuening injection. By adopting the method, the contents of 14 flavonoid glycoside compounds and 2 phenolic acid compounds in the Shuxuening injection can be simultaneously determined, and the new analysis method and a basis are provided for quality control of commercially available Shuxuening injections.

Description

technical field [0001] The invention relates to the field of drug detection, more specifically, the invention relates to a high-performance liquid chromatography detection method for simultaneously determining the contents of multiple components in Shuxuening injection. Background technique [0002] Shuxuening Injection is clinically used for the treatment of cardiovascular diseases and peripheral circulatory disorders, and works through multiple targets and multiple links. It can reduce the cardiovascular risk caused by high homocysteine ​​(Hcy); reduce the pro-inflammatory factor plasma C-reactive protein (CRP) related to the occurrence, evolution and development of atherosclerosis; reduce the strong vasoconstriction and Thromboxane B2 (TXB2) that promotes platelet aggregation; reduces serum lipid peroxide (LOP), which can cause various damages to the structure and function of cells and cell membranes; reduces endogenous vasoconstrictor activity widely present in various t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 常艳旭高秀梅田吉李晋王晖李志刚张纲郝福关秀伟
Owner HEBEI SHINEWAY PHARMA
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