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Verticillium chlamydosporium microbial inoculant, and protective agent and preparation method thereof

A technology of Verticillium chlamydoides and bacterial agents, which is applied in the field of microbial bacterial agents, can solve problems such as difficulty in preservation of Verticillium chlamydia, decline in effective viable bacteria, and difficulties in biological control, and achieve a simple and easy-to-operate preparation method, enhanced The effect of stability and low cost

Active Publication Date: 2017-05-24
湖南慧谷农业生态研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in order to commercialize Verticillium chlamydia, it is necessary to increase its validity period. Shi Yan’s research shows that the effective viable bacteria will decrease after 90 days at 4°C when the spore powder of Verticillium chlamydia is made into wettable powder. 30%, and it decreased by 75.38% when stored at room temperature
The difficult preservation of Verticillium chlamydia makes it difficult to use it for biological control. Therefore, it is very meaningful to develop a more effective protective agent that can make the effective period of Verticillium chlamydia longer

Method used

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  • Verticillium chlamydosporium microbial inoculant, and protective agent and preparation method thereof
  • Verticillium chlamydosporium microbial inoculant, and protective agent and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0027] The preparation of embodiment 1 Verticillium chlamydia agent (1)

[0028] Verticillium chlamydia liquid fermentation medium is potato medium (PS medium), culture temperature is 25-28 ℃, rotation speed is 120r / min, air flow is 2.5kg, culture time is 7 days. The fermented liquid was centrifuged with a disc centrifuge at a speed of 6000r / min. Mix the obtained mycelia with a protective agent, including 42kg of zeolite powder, 20kg of humic acid, 36kg of corn starch, 1.2kg of sucrose, 1kg of sodium chloride, 0.5kg of Tween 80, 0.01kg of chloramphenicol, and Verticillium chlamydia Fungal mycelium 25.18kg. Adjust the moisture to about 20%, granulate with a double-mode rapid granulator, dry at 30°C after granulation, and dry until the moisture is below 8%.

Embodiment 2

[0029] The preparation of embodiment 2 Verticillium chlamydia agent (2)

[0030] The liquid fermentation medium of the Verticillium chlamydia preparation is potato medium (PS medium), the cultivation temperature is 25-28° C., the rotation speed is 120 r / min, the ventilation volume is 2.5 kg, and the cultivation time is 5 days. The fermented liquid was centrifuged with a disc centrifuge at a speed of 6000r / min. Mix the obtained hyphae with a protective agent, including 30kg of zeolite powder, 15kg of humic acid, 25kg of corn starch, 0.2kg of sucrose, 0.1kg of sodium chloride, 0.1kg of Tween 80, 0.01kg of chloramphenicol, and 17.60 kg of mycelia of mycobacteria. Adjust the moisture to about 20%, granulate with a double-mode rapid granulator, dry at 30°C after granulation, and dry until the moisture is below 8%.

Embodiment 3

[0031] The preparation of embodiment 3 Verticillium chlamydia agent (3)

[0032] The liquid fermentation medium of the Verticillium chlamydia preparation is potato medium (PS medium), the culture temperature is 25-28°C, the rotation speed is 120r / min, the ventilation rate is 2.5kg, and the culture time is 6 days. The fermented liquid was centrifuged with a disc centrifuge at a speed of 6000r / min. Mix the obtained mycelia with a protective agent, including 50kg of zeolite powder, 25kg of humic acid, 45kg of corn starch, 2kg of sucrose, 1.5kg of sodium chloride, 1.2kg of Tween 80, 0.01kg of chloramphenicol, and Verticillium chlamydia Fungal mycelium 31.18kg. Adjust the moisture to about 20%, granulate with a double-mode rapid granulator, dry at 30°C after granulation, and dry until the moisture is below 8%.

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Abstract

The invention provides a Verticillium chlamydosporium microbial inoculant, and a protective agent and a preparation method thereof. The microbial inoculant contains the protective agent. The protective agent contains the following raw materials in parts by weight: 30-50 parts of zeolite powder, 15-25 parts of humic acid, 25-45 parts of corn starch, 0.2-2 parts of sucrose, 0.1-1.5 parts of sodium chloride, 0.1-1.2 parts of Tween 80 and 0.01 part of chloramphenicol. The preparation method comprises the following steps: uniformly mixing Verticillium chlamydosporium hyphae and the protective agent, granulating by a double-mold quick granulator, and drying to obtain the Verticillium chlamydosporium microbial inoculant. The protective agent can effectively prolong the effective period of Verticillium chlamydosporium. The prepared Verticillium chlamydosporium microbial inoculant has the advantages of favorable stability, long expiration date, easy preservation and low efficacy loss tendency, and can be widely used for treating root knot nematode disease of crops and promoting crop growth.

Description

technical field [0001] The invention relates to a microbial bacterial agent, in particular to a Verticillium chlamydia agent for biological control, its protective agent and a preparation method. Background technique [0002] Verticillium chlamydoides, also known as Pochonia chlamydosporia, belongs to Deuteromycotina, Hyphomycetes, Hyphomycetes, Moniliales ), Hyphaceae (Monilaccae), and Pochonia. [0003] Root-knot nematode is the pathogen that causes crop root-knot nematode. Its host range is very wide, and it is prevalent in tropical and subtropical regions. It brings huge losses to the world's crop economy every year. At present, there are many ways to prevent and control root-knot nematode disease. Among them, the biological control method has less impact on humans, animals and the environment, and is the safest way to control nematodes. At present, the most widely used bacteria to control nematodes are Paecilomyces lilacinus and Verticillium chlamydiae. [0004] Rega...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/04C12N1/14A01N63/04A01P5/00A01P21/00C12R1/645
CPCA01N63/30C12N1/04C12N1/14
Inventor 罗志威谭石勇谭武贵丰来徐滔明郑双凤张冬雪
Owner 湖南慧谷农业生态研究院有限公司
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