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Zein/protein-polysaccharide electrostatic complex core/shell type nanocarrier as well as preparation method and application thereof

A technology of zein and nano-carriers, which is applied in the direction of non-active ingredients of polymer compounds, pharmaceutical formulations, microcapsules, etc., can solve the problems of poor stability and nano-carrier stability, and achieve increased concentration, high embedding efficiency, The effect of excellent thermal stability

Active Publication Date: 2017-05-24
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The concentration of the polysaccharide used is significantly lower than that of sodium caseinate, but the influence of the polysaccharide on the stability of the nanocarrier is obviously affected by the surface charge of the polysaccharide and the pH of the water phase. Stable, but when sodium alginate is used as a stabilizer, the stability is poor at pH6.5~7.0 and pH3.0
At the same time, we used alcohol-soluble polyphenols as embedding objects, and the stability of the prepared nanocarriers after heating was poor. After heating at 70°C for 10 minutes, the particles aggregated and precipitated.

Method used

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  • Zein/protein-polysaccharide electrostatic complex core/shell type nanocarrier as well as preparation method and application thereof
  • Zein/protein-polysaccharide electrostatic complex core/shell type nanocarrier as well as preparation method and application thereof
  • Zein/protein-polysaccharide electrostatic complex core/shell type nanocarrier as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0029] (1) Preparation of zein nanocarriers loaded with curcumin: Curcumin and zein were dissolved in 85% (v / v) ethanol-water solution, then injected into water with a pH of 4.0 with a syringe, corn Prolamin ethanol-water solution: the volume ratio range of acidic water is 1:4, while adding zein ethanol-water solution, stir with a magnetic stirrer, the speed of the stirrer is 800 rpm, continue to Stir for 3 minutes, evaporate ethanol with a rotary evaporator, and add the same volume of acidic water to replenish the volume before evaporation to prepare a dispersion of zein nanocarriers. The particle size of the nanocarriers is about 60-80nm.

[0030] (2) Preparation of protein-polysaccharide electrostatic complex: prepare pectin solution with a concentration of 0.2% (w / v) and gelatin solution with a concentration of 0.2% (w / v) (by heating and stirring to dissolve), then mix pectin with the same concentration The solution is mixed with the gelatin solution, so that the ratio of ...

Embodiment 2

[0034](1) Preparation of resveratrol-loaded zein nanocarriers: resveratrol and zein were dissolved in 80% (v / v) ethanol-water solution, and then injected with a syringe to pH 4.0 In the water, the ethanol-water solution of zein: the volume ratio range of acidic water is 1:4, while adding the ethanol-water solution of zein, stir with a magnetic stirrer, the speed of the stirrer is 1200 rpm, Continue to stir for 3 minutes after complete addition, remove ethanol by evaporation with a rotary evaporator, and add the same volume of acidic water to replenish the volume before evaporation to obtain a dispersion of zein nano-carriers. The particle size of the nano-carriers is about 60 ~ 80nm.

[0035] (2) Preparation of protein-polysaccharide electrostatic complex: preparation of 0.2% (w / v) pectin solution and 0.2% (w / v) sodium caseinate solution (pectin and sodium caseinate solution are dispersed in water, heated and stirred to dissolve), then mix the same concentration of pectin sol...

Embodiment 3

[0039] (1) Preparation of resveratrol-loaded zein nanocarriers: resveratrol and zein were dissolved in 85% (v / v) ethanol-water solution, and then injected into pH4 water with a syringe , Ethanol-water solution of zein: the volume ratio range of acidic water is 1:5, while adding the ethanol-water solution of zein, stir with a magnetic stirrer, the speed of the stirrer is 1000 rpm, fully add Then continue to stir for 3 minutes, remove ethanol by evaporation with a rotary evaporator, and add the same volume of acidic water to replenish the volume before evaporation to obtain a dispersion of zein nanocarriers. The particle size of the nanocarriers is about 60-80nm.

[0040] (2) Preparation of protein-polysaccharide electrostatic complex: prepare sodium alginate solution and 0.15% (w / v) sodium caseinate solution with a concentration of 0.15% (w / v) (by heating, dispersing, stirring and dissolving), and then Mix pectin solution and gelatin solution of the same concentration, so that ...

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Abstract

The invention discloses a zein / protein-polysaccharide electrostatic complex core / shell type nanocarrier which is a nano microsphere, wherein zein serves as a core, and the exterior is coated with a protein-polysaccharide electrostatic complex to be served as a shell. The invention also discloses a preparation method and application of the nanocarrier. The nanocarrier has the advantages of narrow particle size distribution range and good pH stability and heat resistance and can be used for embedding drugs soluble to ethanol, food functional ingredients and phytochemical substances so as to promote the solubility in water, improve the stability and bioavailability of the embedded ingredients and enhance the effect or healthcare function of the ingredients.

Description

technical field [0001] The invention relates to a natural biopolymer nanocarrier, in particular to a zein / protein-polysaccharide electrostatic complex core / shell nanocarrier, and also relates to a preparation method and application of the nanocarrier. Background technique [0002] Some weakly polar drugs (such as anticancer drugs) and food functional ingredients (such as alcohol-soluble polyphenols) can be dissolved in ethanol, but their solubility in water is very low, which affects the bioavailability and biological activity after oral administration. Its application in medicine and health food. Dissolving these functional components of drugs or food together with zein in ethanol solution and preparing zein nanocarriers loaded with these components by anti-solvent method is an effective way to improve its solubility and bioavailability in water. way. However, zein is a highly hydrophobic protein. In the aqueous system, zein nanocarriers aggregate due to hydrophobic inter...

Claims

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Application Information

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IPC IPC(8): A61K47/42A61K47/36A61K9/50
CPCA61K9/50A61K47/36A61K47/42
Inventor 胡坤
Owner GUANGDONG PHARMA UNIV
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